Patents by Inventor Folke Tjerneld
Folke Tjerneld has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7553658Abstract: The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.Type: GrantFiled: June 17, 2003Date of Patent: June 30, 2009Assignee: GE Healthcare Bio-Sciences ABInventors: Cecilia Jansson Kepka, Jenny Rhodin Edsō, Folke Tjerneld
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Patent number: 7335492Abstract: The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.Type: GrantFiled: October 19, 2005Date of Patent: February 26, 2008Assignee: Valtion Teknillinen TutkimuskeskusInventors: Merja Penttilä, Tiina Nakari-Setälä, Richard Fagerström, Klaus Selber, Maria-Regina Kula, Markus Linder, Folke Tjerneld
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Patent number: 7193056Abstract: The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step.Type: GrantFiled: June 8, 2004Date of Patent: March 20, 2007Assignee: Pharmacia & UpJohn ABInventors: Hans Ageland, Lena Nyström, Josefine Persson, Folke Tjerneld
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Publication number: 20060166349Abstract: The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.Type: ApplicationFiled: June 17, 2003Publication date: July 27, 2006Inventors: Cecilia Kepka, Jenny Rhodin, Folke Tjerneld
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Patent number: 7060669Abstract: The present invention relates to isolation and purification of proteins in aqueous two-phase systems (ATPS). Specifically the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.Type: GrantFiled: March 24, 2000Date of Patent: June 13, 2006Assignee: Valtion Teknillinen TutkimuskeskusInventors: Merja Penttilä, Tiina Nakari-Setälä, Richard Fagerström, Klaus Selber, Maria-Regina Kula, Markus Linder, Folke Tjerneld
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Publication number: 20060084164Abstract: The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.Type: ApplicationFiled: October 19, 2005Publication date: April 20, 2006Inventors: Merja Penttila, Tiina Nakari-Setala, Richard Fagerstrom, Klaus Selber, Maria-Regina Kula, Markus Linder, Folke Tjerneld
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Patent number: 6767994Abstract: The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step.Type: GrantFiled: May 16, 2000Date of Patent: July 27, 2004Assignee: Pharmacia ABInventors: Hans Ageland, Lena Nyström, Josefine Persson, Folke Tjerneld
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Patent number: 6559284Abstract: The present invention relates to a composition for use in purification of apolipoprotein A (ApoA) or apolipoprotein E (ApoE), said composition comprising a first and a second polymeric material, wherein the first and second polymeric material are immiscible in the primary aqueous solution, and wherein the second polymeric material is amphiphilic and water soluble. The invention further relates to a process for purifying ApoA or ApoE, or variants or mixtures thereof, by first mixing ApoA or ApoE, the composition containing a first and second polymeric material and water. The resulting primary aqueous solution is maintained for a period of time sufficient for essentially separating the phases formed, and removing the phase containing the second polymeric material and the main portion of ApoA or ApoE. Subsequently, the second polymeric material is separated from ApoA or ApoE.Type: GrantFiled: September 5, 1997Date of Patent: May 6, 2003Assignee: Esperion Therapeutics, Inc.Inventors: Hans Ageland, Lena Nyström, Josefine Persson, Folke Tjerneld
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Publication number: 20030049819Abstract: A method for separating one or more hydrophobic proteins, for instance membrane proteins such as integral membrane proteins, from a mixture of proteins. The method is characterized in that said mixture is partitioned in a phase system comprising a micelle-enriched aqueous phase (micelle-phase) and a polymer-enriched aqueous phase (polymer phase). At least part of the polymer of the polymer phase carries an affinity ligand that is capable of binding to an affinity structure on at least one of said one or more hydrophobic proteins.Type: ApplicationFiled: September 3, 2002Publication date: March 13, 2003Inventors: Folke Tjerneld, Ulf Sivars
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Patent number: 6454950Abstract: A method for the separation of a predetermined compound comprising i) partitioning the compound in a two-phase (system A) in which at least one of the phases is rich (phase 1) and the other is poor (phase 2) in a thermoseparating polymer (I), ii) collecting one phase (phase 1 or phase 2) containing said compound, and iii if desired, further working up said compound from the phase collected in step ii, characterized a) in that polymer (I) is a micell-forming thermoseparating polymer, and b) in that phase 2 possibly contains at least one agent that is cloud point-decreasing for thermoseparating polymer (I), such as a polymer (II) that is incompatible with polymer (I) or a salt.Type: GrantFiled: October 3, 2001Date of Patent: September 24, 2002Assignee: Amersham Pharmacia Biotech ABInventors: Folke Tjerneld, Josefine Persson, Hans Olof Johansson
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Patent number: 6130075Abstract: The invention provides novel polymer conjugates that are especially useful in the separation of carboxylic acids from complex fermentation compositions. The polymer conjugates comprise polyethylene glycols or oxides and polyethyleneimine or polypropyleneimine in various ratios. The novel polymer conjugates permit enhanced extraction of carboxylic acids from fermentation systems not only through their use in partitioning the carboxylic acids from various polymeric components of the fermentation system, but also through their use in adjusting the pH of the system for optimal performance and separation.Type: GrantFiled: April 22, 1999Date of Patent: October 10, 2000Assignee: Forskarpatent I Syd ABInventors: Jordi Planas, Folke Tjerneld, Barbel Hahn-Hagerdal
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Patent number: 6107467Abstract: The present invention relates to a process for purifying a hydrophobic or amphiphilic compound, by first mixing a starting material containing the hydrophobic or amphiphilic compound with a first polymeric material, water and at least one of a second polymeric material and a surfactant, wherein the first polymeric material and the second polymeric material and/or surfactant are immiscible in the resulting primary aqueous solution. The process further comprises maintaining the primary aqueous solution for a period of time sufficient for essentially separating the phases formed, and then removing the phase containing the main portion of the hydrophobic or amphiphilic compound and the second polymeric material and/or surfactant. The second polymeric material and/or surfactant are separated from the hydrophobic or amphiphilic compound, and subsequently recycled to the initial mixing step.Type: GrantFiled: September 5, 1997Date of Patent: August 22, 2000Assignee: Pharmacia & Upjohn ABInventors: Hans Ageland, Lena Nystrom, Josefine Persson, Folke Tjerneld