Abstract: The invention relates to a sample holder assembly (1) for microscopy comprising at least the following components: A first member (10) having a first opening (14) on a first side of the first member (10), and a circumferential wall portion (12) having an inward facing side (12-1) that faces toward the first volume (V1) and an outward facing side (12-2) opposite the inward facing side (12-1); A second member (20) having a first opening (24) at a first side of the second member (20), and a circumferential wall portion (22) having an inward facing side (22-1) facing toward the second volume (V2), wherein a shape of the outward facing side (12-2) of the wall portion (12) of the first member (10) is complementary to the inward facing side (22-1) of the wall portion (22) of the second member (20), such that the wall portion (12) of the first member (10) can be inserted in the second volume (V2), such that the first volume (V1) is at least partially comprised by the second volume (V2); A transparent membrane (30) ha