Patents by Inventor Françoise Debart
Françoise Debart has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9035041Abstract: This invention relates to a process for preparing an oligonucleotide 5?-triphosphate.Type: GrantFiled: December 22, 2009Date of Patent: May 19, 2015Assignee: Alnylam Pharmaceuticals, Inc.Inventors: Ivan Zlatev, Francois Morvan, Jean-Jacques Vasseur, Francoise Debart, Muthiah Manoharan
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Patent number: 8536318Abstract: The invention relates to a method for the chemical synthesis of RNA, comprising the following steps: a) bonding to a solid support of a monomer having formula (II) in which—X1 is a dimethoxytrityl group,—X6 is H or an OAc group or OX3, in which X3 is a group having formula (A), in which X is O or S, R? is H or CH3 and R is selected from a linear or branched alkyl group at C1 to C4 and a R1—O—R2 group in which R1 is an alkyl group at C1 to C2 and R2 is a CH3 group or CH2CH2—O—CH3 or aryl; b) assembly with the monomer having formula (II) bound to the support thereof obtained in step (a) of at least one monomer having formula (III) in which X1, Bp, X3 are as defined for formula (II) and X5 is a hydrogen phosphonate monoester or phosphoramidite group, preferably a 2-cyanoethyl-N,N-diisopropylphosphoramidite group, which is used to obtain a protected single-strand RNA bound to a support.Type: GrantFiled: May 28, 2009Date of Patent: September 17, 2013Assignees: Centre National de la Recherche Scientifique, Universite Montpellier 2Inventors: Françoise Debart, Jean-Jacques Vasseur, Thomas Lavergne
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Patent number: 8304532Abstract: A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3?-protected compound of formula: with a nucleotide derivative having a 5?-protection group, or reacting a 5?-protected compound of formula with a nucleotide derivative having a 3?-protection group.Type: GrantFiled: May 25, 2011Date of Patent: November 6, 2012Assignees: Girindus AG, Centre National de la Recherche Scientifique, University of Montpellier IIInventors: Ilaria Adamo, Cecile Dueymes, Andreas Schonberger, Jean-Louis Imbach, Albert Meyer, Francois Morvan, Francoise Debart, Jean-Jacques Vasseur, Meinolf Lange, Fritz Link
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Publication number: 20120220761Abstract: The present invention describes simple, efficient, and enzyme-free method of making oligonucleotides with 5?-triphosphate. This invention presents novel process for synthesizing triphosphate oligonucleotides using a diaryl phosphonate as reagent. The process of the present invention is amenable to large-scale, economic 5?-triphosphate oligonucleotide synthesis.Type: ApplicationFiled: December 22, 2009Publication date: August 30, 2012Applicant: ALNYLAM PHARMACEUTICALS, INC.Inventors: Ivan Zlatev, Francois Morvan, Jean-Jacques Vasseur, Francoise Debart, Muthiah Manoharan
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Publication number: 20110275793Abstract: The invention relates to a method for the chemical synthesis of RNA, comprising the following steps: a) bonding to a solid support of a monomer having formula (II) in which—X1 is a dimethoxytrityl group,—X6 is H or an OAc group or OX3, in which X3 is a group having formula (A), in which X is O or S, R? is H or CH3 and R is selected from a linear or branched alkyl group at C1 to C4 and a R1—O—R2 group in which R1 is an alkyl group at C1 to C2 and R2 is a CH3 group or CH2CH2—O—CH3 or aryl; b) assembly with the monomer having formula (II) bound to the support thereof obtained in step (a) of at least one monomer having formula (III) in which X1, Bp, X3 are as defined for formula (II) and X5 is a hydrogen phosphonate monoester or phosphoramidite group, preferably a 2-cyanoethyl-N,N-diisopropylphosphoramidite group, which is used to obtain a protected single-strand RNA bound to a support.Type: ApplicationFiled: May 28, 2009Publication date: November 10, 2011Inventors: Françoise Debart, Jean-Jacques Vasseur, Thomas Lavergne
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Publication number: 20110224424Abstract: A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large- scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3?-protected compound of formula: with a nucleotide derivative having a 5?-protection group, or reacting a 5?-protected compound of formula with a nucleotide derivative having a 3?-protection group.Type: ApplicationFiled: May 25, 2011Publication date: September 15, 2011Applicants: GIRINDUS AG, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITY OF MONTPELLIER IIInventors: Ilaria ADAMO, Cécile DUEYMES, Andreas SCHÖNBERGER, Jean-Louis IMBACH, Albert MEYER, Francois MORVAN, Francoise DEBART, Jean-Jacques VASSEUR, Meinolf LANGE, Fritz LINK
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Publication number: 20100069623Abstract: A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3?-protected compound of formula: with a nucleotide derivative having a 5?-protection group, or reacting a 5?-protected compound of formula with a nucleotide derivative having a 3?-protection group.Type: ApplicationFiled: April 3, 2009Publication date: March 18, 2010Applicants: GIRINDUS AG, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITY OF MONTPELLIER IIInventors: Ilaria Adamo, Cècile Dueymes, Andreas Schönberger, Jean-Louis Imbach, Albert Meyer, Francois Morvan, Francoise Debart, Jean-Jacques Vasseur, Meinolf Lange, Fritz Link
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Publication number: 20060089494Abstract: A method for preparing an oligonucleotide comprising the steps of a) providing a 3-protected compound having the formula: wherein B is a heterocyclic base R2 is H, a protected 2-hydroxyl group, F, a protected amino group, an O-alkyl group, an O-substituted alkyl, a substituted alkylamino or a C4?-O2?methylen linkage R3 is OR?3, NHR?3, NR?3R??3, a 3?-protected nucleotide or a 3?-protected oligonucleotide, R?3 is a hydroxyl protecting group, R?3, R??3 are independently an amine protecting group, b) reacting said compound with a nucleotide derivative having a 5-proctection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond c) optionally processing the elongated oligonucleotide with a P(III)-internucleotide bond by either or both of steps c1) and c2) in any sequence c1) capping preferably by reacting with a solid supported capping agent c2) oxidizing preferably by reacting the oligonucleotide with a solid supported oxidizing reagent d) remoType: ApplicationFiled: July 30, 2003Publication date: April 27, 2006Applicants: Girindus AG, Centre National De La Recherche Scientifique, University of Montpellier IIInventors: Ilaria Adamo, Cecile Dueymes, Andreas Schonberger, Jean-Louis Imbach, Albert Meyer, Francois Morvan, Francoise Debart, Jean-Jacques Vasseur, Meinolf Lange, Fritz Link
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Patent number: 6900301Abstract: Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.Type: GrantFiled: May 22, 2002Date of Patent: May 31, 2005Assignee: ISIS Pharmaceuticals, Inc.Inventors: Phillip Dan Cook, Yogesh Shantilal Sanghvi, Jean Jacques Vasseur, Francoise Debart
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Publication number: 20030045705Abstract: Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.Type: ApplicationFiled: May 22, 2002Publication date: March 6, 2003Applicant: ISIS Pharmaceuticals, Inc.Inventors: Phillip Dan Cook, Yogesh Shantilal Sanghvi, Jean Jacques Vasseur, Francoise Debart
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Patent number: 5965721Abstract: Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.Type: GrantFiled: December 11, 1996Date of Patent: October 12, 1999Assignee: Isis Pharmaceuticals, Inc.Inventors: Phillip Dan Cook, Yogesh Shantilal Sanghvi, Jean Jacques Vasseur, Francoise Debart
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Patent number: 5610289Abstract: Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly- nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.Type: GrantFiled: April 7, 1994Date of Patent: March 11, 1997Assignee: Isis Pharmaceuticals, Inc.Inventors: Phillip D. Cook, Yogesh S. Sanghvi, Jean J. Vasseur, Francoise Debart