Abstract: The invention provides a light generating device (1000) comprising a light source (10) and a luminescent element (20), wherein:—the light source (10) is configured to generate the first radiation (11); wherein the light source (10) comprises a laser light source;—the luminescent element (20) comprises (i) a plurality of element bodies (200) and (ii) a thermally conductive support (400); wherein the plurality of element bodies (200) comprises a plurality of first bodies (210) and a plurality of second bodies (220);—the plurality of first bodies (210) comprise a luminescent material (50), wherein the luminescent material (50) is configured to convert at least part of first radiation (11), selected from one or more of UV radiation and visible radiation, into luminescent material light (51); wherein the first bodies have a first thermal conductivity K1; wherein the first bodies (210) are configured in a light receiving relationship with the light source (10);—the plurality of second bodies (220), different from t

Abstract: A light generating device (100) comprising a plurality of solid state light sources (10) and a housing (120) comprising side wall elements (20). The light sources (10) are enclosed by the side wall elements (20), and the light generating device (100) generates >=90% of light source light (11) within a triangular prism having a top angle (?)<=180°. The side wall elements (20): —each have a first side (21) directed inwards facing the light sources (10) and a second side (22) which is directed outwards and is reflective for visible light;—are configured at both sides of a housing plane (110), under an angle (?1) between 0-45° relative to the housing plane (110);—have a projection with height H1 on the housing plane (110)—define a largest width (W1) of the light housing 120), wherein H1/W1>1.

Abstract: The invention provides a photoreactor assembly (1) comprising a reactor (30), wherein the reactor (30) is configured for hosting a fluid (100) to be treated with light source radiation (11) selected from one or more of UV radiation, visible radiation, and IR radiation, wherein the reactor (30) comprises a reactor wall (35) which is transmissive for the light source radiation (11), wherein the photoreactor assembly (1) further comprises: a light source arrangement (1010) comprising a plurality of light sources (10) configured to generate the light source radiation (11), wherein the reactor wall (35) is configured in a radiation receiving relationship with the plurality of light sources (10); one or more fluid transport channels (7) configured in functional contact with one or more of (i) the reactor (30) and (ii) one or more of the plurality of light sources (10); a cooling system (90) configured to transport a cooling fluid (91) through the one or more fluid transport channels (7).

Abstract: The invention provides a reactor assembly (1) comprising a reactor (30), wherein the reactor (30) is configured for hosting a fluid (100) to be treated with light source radiation (11) selected from one or more of UV radiation, visible radiation, and IR radiation, wherein the reactor (30) comprises a reactor wall (35) which is transmissive for the light source radiation (11), wherein: (i) the reactor (30) is a tubular reactor (130), and wherein the reactor wall (35) defines the tubular reactor (130); (ii) the tubular reactor (130) is configured in a tubular arrangement (1130); and (iii) the reactor assembly (1) further comprises a reactor support element (40), wherein (a) the reactor support element (40) encloses at least part of the tubular arrangement (1130) or wherein (b) the tubular arrangement (1130) encloses at least part of the reactor support element (40); wherein part of the tubular arrangement (1130) is configured in contact with the reactor support element (40), and wherein another part of the tubu

Abstract: A method of selecting a monoclonal cell colony that secretes a product of interest providing a plurality of cell culture spaces; each cell culture space has a respective assessment surface with a first binding agent immobilized on the assessment surface. The cells are incubated in a growth medium in the cell culture spaces for replication of the cells. At least some of the cells secrete a product of interest. A second binding agent is provided in the cell culture spaces. Initially, the second binding agent is not immobilized. Each of the first and second binding agents binds to the product of interest so that secretion of the product of interest by cells in any one of the cell culture spaces leads to a complex with the first binding agent, the product of interest and the second binding agent forming at the corresponding assessment surface of the cell culture space.

Abstract: A method of selecting a monoclonal cell colony that secretes a product of interest providing a plurality of cell culture spaces; each cell culture space has a respective assessment surface with a first binding agent immobilised on the assessment surface. The cells are incubated in a growth medium in the cell culture spaces for replication of the cells. At least some of the cells secrete a product of interest. A second binding agent is provided in the cell culture spaces. Initially, the second binding agent is not immobilised. Each of the first and second binding agents binds to the product of interest so that secretion of the product of interest by cells in any one of the cell culture spaces leads to a complex with the first binding agent, the product of interest and the second binding agent forming at the corresponding assessment surface of the cell culture space.

Abstract: In one aspect the present invention provides a method of identifying a cell or cell colony which produces a polypeptide of interest, the method comprising exposing one or more cells to a marker compound which associates with a reference polypeptide, wherein production of the polypeptide of interest by the cells is linked to production of the reference polypeptide, and detecting association of the marker compound with the one or more cells, thereby identifying a cell or cell colony which produces the polypeptide of interest.

Abstract: The present invention provides in one aspect a method for determining a production level of a polypeptide of interest by a cell or cell colony, comprising obtaining an image of one or more cells or cell colonies; and analyzing the image to detect a signal associated with the polypeptide of interest; wherein a signal level is determined for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.

Abstract: We describe a method of growing an animal cell in a culture medium, in which the culture medium comprises an elevated concentration of a thymidine family member, in which the growth or viability of the animal cell is increased as a result of the elevated concentration of the thymidine family member in the cell culture medium. Preferably, the cell culture medium comprises a semi-solid medium, which is a serum free or chemically defined medium.

Abstract: A hydraulic system controls engagement of a transfix roller with an image receiving member in a printer.

Abstract: The present invention is a breakaway mounting bracket assembly for a wiper system that displaces components of the wiper system below the impact line of a vehicle in the event of, for example, a vehicle-pedestrian impact. The assembly includes a mounting bracket and a grommet removably attached to the mounting bracket. The assembly also includes a ferrule disposed within the grommet that includes a column having an internal passage and a platform connected to the column by a plurality of bridges disposed therebetween. The assembly also includes an attachment member to connect the mounting bracket to the vehicle where the attachment member includes a body engaged to the internal passage. The mounting bracket is adapted to break away from attachment with the grommet in the event of a predetermined load acting on a pivot shaft assembly and to displace the pivot shaft assembly and a portion of the wiper arm below the vehicle's impact line in the event of a pedestrian impact.

Abstract: The present invention is a breakaway mounting bracket assembly for a wiper system that displaces components of the wiper system below the impact line of a vehicle in the event of, for example, a vehicle-pedestrian impact. The assembly includes a mounting bracket and a grommet removably attached to the mounting bracket. The assembly also includes a ferrule disposed within the grommet that includes a column having an internal passage and a platform connected to the column. The assembly also includes an attachment member to connect the mounting bracket to the vehicle where the attachment member includes a body engaged to the internal passage. The platform breaks away from the column in the event of a predetermined load acting on the pivot shaft assembly, which disconnects the grommet from the ferrule and thereby displaces the pivot shaft assembly and a portion of a wiper arm below the impact line of a vehicle.

Abstract: The present invention is a breakaway mounting bracket assembly for a wiper system that displaces components of the wiper system below the impact line of a vehicle in the event of, for example, a vehicle-pedestrian impact. The assembly includes a mounting bracket and a grommet removably attached to the mounting bracket. The assembly also includes a ferrule disposed within the grommet that includes a column having an internal passage and a platform connected to the column by a plurality of bridges disposed therebetween. The assembly also includes an attachment member to connect the mounting bracket to the vehicle where the attachment member includes a body engaged to the internal passage. The mounting bracket is adapted to break away from attachment with the grommet in the event of a predetermined load acting on a pivot shaft assembly and to displace the pivot shaft assembly and a portion of the wiper arm below the vehicle's impact line in the event of a pedestrian impact.

Abstract: The present invention provides in one aspect a method for determining a production level of a polypeptide of interest by a cell or cell colony, comprising obtaining an image of one or more cells or cell colonies; and analyzing the image to detect a signal associated with the polypeptide of interest; wherein a signal level is determined for each cell or cell colony based on signal values from a predefined area surrounding the cell or cell colony, the signal level being indicative of the production level of the polypeptide of interest by the cell or cell colony.

Abstract: In one aspect the present invention provides a method of identifying a cell or cell colony which produces a polypeptide of interest, the method comprising exposing one or more cells to a marker compound which associates with a reference polypeptide, wherein production of the polypeptide of interest by the cells is linked to production of the reference polypeptide, and detecting association of the marker compound with the one or more cells, thereby identifying a cell or cell colony which produces the polypeptide of interest.

Abstract: A hydraulic system controls engagement of a transfix roller with an image receiving member in a printer.

Abstract: In one embodiment, an apparatus includes a housing that has at least an inlet and an outlet. The housing is configured to receive a volume of fluid via the inlet. The volume of fluid is in a substantially liquid state and at least a portion of the volume of fluid includes a dissolved impurity. The apparatus also includes a heat-transfer element coupled to an interior volume of the housing. The heat-transfer element includes a surface, at least a portion of which is disposed at an angle with respect to a horizontal plane. The volume of fluid includes a surface parallel to the horizontal plane. The apparatus further includes a compression component configured to compress at least a portion of fluid boiled from the volume of fluid.

Abstract: In one embodiment, a method includes receiving a signal from a sensor disposed within a housing. The housing includes a boiler portion and a condenser portion. At least a portion of the boiler portion and at least a portion of the condenser portion are defined by a heat-transfer element coupled to an interior portion of the housing. The method also includes modifying an angle of the heat-transfer element relative to a horizontal plane in response to the signal such that at least one of a heat-transfer rate associated with the heat-transfer element or a flow-rate of a fluid changing phase within the housing is modified.

Abstract: An insulation displacement electrical connector assembly is disclosed for electrically terminating the conductors of a multi-conductor insulated flat cable. A connector base has a plurality of terminal-receiving passages. A connector cover is positionable on the base to provide a cable-receiving passage therebetween. The cable-receiving passage traverses the terminal-receiving passages. Complementary interengaging latches are provided between the base and the cover to hold the base and cover together clamping the cable in the cable-receiving passage. A plurality of insulation displacement terminals are movably mounted in the terminal-receiving passages for movement between inactive positions out of engagement with the conductors of the cable and insulation-displacement positions piercing the insulation of the cable and electrically terminating the conductors.