Abstract: A tracheo-bronchial sampler device comprises a tubular member insertable into a chest tube and defining a passage through which a sampling catheter can be passed when the chest tube is inserted into the trachea of a patient, means are provided for spacing the passage of said tubular member away from the inner wall of said chest tube, as well as stop means for preventing the insertion of the tubular member through the chest tube beyond a predetermined point. The device enables a sample to be obtained from the desired tracheal region without undesired contamination as the sampling catheter is inserted into the patient.