Abstract: A medium is analyzed for the search for an odorous biological molecule characteristic of a specific substance in an environment to be studied comprising the steps of: exposing a component containing at least one olfactory biological receptor, capable of selectively attaching the type of odorous molecule sought, to an atmosphere taken from the environment to be studied; irradiating that component by means of an incident laser radiation having a wavelength chosen in the ultraviolet close to the absorption wavelength for the olfactory receptor, to cause the excitation of a resonance Raman scattering in the irradiated component; and analyzing the Raman spectrum obtained to determine whether or not it corresponds to the Raman spectrum of the olfactory receptor having attached an odorous molecule of the type sought in the atmosphere sampled, so as to identify any presence of the specific substance mentioned in the environment to be studied.

Abstract: A medium is analysed for the search for an odorous biological molecule characteristic of a specific substance in an environment to be studied comprising the steps of: exposing a component containing at least one olfactory biological receptor, capable of selectively attaching the type of odorous molecule sought, to an atmosphere taken from the environment to be studied; irradiating that component by means of an incident laser radiation having a wavelength chosen in the ultraviolet close to the absorption wavelength for the olfactory receptor, to cause the excitation of a resonance Raman scattering in the irradiated component; and analysing the Raman spectrum obtained to determine whether or not it corresponds to the Raman spectrum of the olfactory receptor having attached an odorous molecule of the type sought in the atmosphere sampled, so as to identify any presence of the specific substance mentioned in the environment to be studied.

Abstract: The invention relates to a Happy Mapping type method for mapping a DNA molecule comprising a step enabling ad infinitum amplification of the DNA of each panel, consisting of the following: (i) a first amplification with the aid of a primer comprising 10-30 nucleotides defined at extremity 5′ thereof, and 5-10 random nucleotides at extremity 3′ thereof, in addition to (ii) a second amplification with the aid of a primer comprising at least the oligonucleotides defined at extremity 5′ of the primer used in stage i.

Abstract: A process produces DNA corresponding to that of the DNA of the virus of B hepatitis. It comprises cloning in bacteria the latter DNA, previously repaired by means of the corresponding precursor nucleotides in the presence of a polymerase. Vectors contain the cloned DNA in their genomes. The cloned DNA is useful as a probe for detecting the presence of the virus of B hepatitis in biological samples, particularly blood or plasma. Its expression in bacteria provides a hybrid protein containing a protein fragment having vaccinating properties against hepatitis B. Nucleic acid of reduced size and a vector containing the nucleotide sequence of which DNA codes an immunogenic peptide sequence capable of inducing the generation of antibodies to the virus of viral hepatitis B. It comprises totally or partly the sequence of nucleotides represented in FIG. 9A.

Abstract: Nucleic acid of reduced size and vector containing said nucleotidic sequence of which DNA codes an immunogenic peptidic sequence capable of inducing the generation of antibodies to the virus of viral hepatitis B. It comprises totally or partly the sequence of nucleotides represented in FIG. 3A. Application to the production by cloning in a bacterium of an immunogenic protein immunizing against hepatitis B, or application to the obtention of probes for the diagnosis of the presence of Dane particles in a serum.

Abstract: Nucleic acid of reduced size and vector containing said nucleotidic sequence of which DNA codes an immunogenic peptidic sequence capable of inducing the generation of antibodies to the virus of viral hepatitis B. It comprises totally or partly the sequence of nucleotides represented in FIG. 3A. Application to the production by cloning in a bacterium of an immunogenic protein immunizing against hepatitis B, or application to the obtention of probes for the diagnosis of the presence of Dane particles in a serum.