Abstract: Contortrostatin, a homodimeric disintegrin, modulates the adhesion, motility, and invasiveness of integrin expressing tumor cells. When formulated as a pharmaceutically acceptable composition, the proteins can be used to treat patients by inhibiting or disrupting disease processes associated with an integrin binding to an &agr;v&bgr;3 or &agr;v&bgr;5 integrin.

Abstract: A size modified fibrinolytic enzyme, wherein the size of the enzyme is modified by covalent attachment of at least one large organic molecule to the enzyme.

Abstract: This invention provides thrombolytic agents and methods for making and using thrombolytic agents. Specifically, the invention provides thrombolytic agents comprising a thrombolytic proteinase and a specific binding peptide that specifically binds at the site of blood clots and thrombi in vivo. The invention particularly provides chemically crosslinked conjugates of a thrombolytic proteinase and a plurality of specific binding peptide. Methods for producing such chemically crosslinked conjugates and methods for using such conjugates for eliminating thrombi in vivo to alleviate pathological conditions caused thereby are also provided. The preferred thrombolytic proteinase in the conjugate is fibrolase obtainable from Agkistrodon contortrix confortrix venom.

Abstract: A disintegrin is employed as an active agent for treatment of various conditions, particularly cancer to prevent metastasis. In a particular embodiment contortrostatin is employed to prevent metastasis in breast cancer patients.

Abstract: Contortrostatin is employed as active agent for treatment of thrombotic disease. Preferably, contortrostatin is employed in conjunction with at least one thrombolytic agent. In addition, contortrostatin is useful in compositions and methods for preventing metastases in carcinoma and melanoma patients, treating or preventing osteoporosis and promoting wound healing.

Abstract: Novel fibrinolytic zinc metalloproteinases exhibiting direct fibrinolytic activity in plasminogen free systems, no detectable plasminogen activation activity in vitro, and no observable systemic toxicity in vivo as exemplified by the absence of hemorrhagic activity, characterized by peptide bond cleavage specificities which are markedly different from those of the heretofore identified metalloproteinases. Several of the enzymes are further characterized by substantially reduced proteolytic activity (as measured by azocaseinolytic activity in vitro) relative to A.c.contortrix fibrolase. The novel fibrinolytic enzymes with reduced proteolytic activity generally exhibit levels of proteolytic activity less than about 70% of that exhibited by a fibrinolytic enzyme having the activity profile of the enzyme as isolated from A.c.contortrix venom.

Abstract: A protease moiety having direct fibrinolytic activity which is useful in thrombolytic therapy is isolated from snake venom and shown to comprise a metalloproteinase having a molecular weight of from 25 to 27 kd and an isoelectric point of about 6.5 to 7.0. The moiety is separated from venom by a series of diverse fractionation steps including molecular sieve, ion-exchange and affinity chromatography.