Abstract: The invention relates to an unspecific peroxygenase of the Agrocybe aegerita fungus, obtained by means of directed molecular evolution to facilitate the functional expression thereof in an active, soluble and stable form. The peroxygenase described in the invention shows a significant improvement in the functional expression thereof, improved monooxygenase activity and reduced peroxidase activity, in relation to the monooxygenase and peroxidase activities showed by the unspecific wild-type peroxygenase of A. aegerita. The peroxygenase of the invention is useful in chemical processes, including industrial transformations such as the selective oxyfunctionalisation of carbon-hydrogen bonds of various organic compounds.

Abstract: The invention relates to an unspecific peroxygenase of the Agrocybe aegerita fungus, obtained by means of directed molecular evolution to facilitate the functional expression thereof in an active, soluble and stable form. The peroxygenase described in the invention shows a significant improvement in the functional expression thereof, improved monooxygenase activity and reduced peroxidase activity, in relation to the monooxygenase and peroxidase activities showed by the unspecific wild-type peroxygenase of A. aegerita. The peroxygenase of the invention is useful in chemical processes, including industrial transformations such as the selective oxyfunctionalisation of carbon-hydrogen bonds of various organic compounds.

Abstract: An industrially viable process of obtaining prebiotic oligosaccharides using a new Xanthophyllomyces dendrorhous enzyme, characterized by showing ?-glucosidase activity is provided. A process of obtaining an enzymatic product with ?-glucosidase activity as well as the substantially pure enzyme with ?-glucosidase activity, are also provided. The enzymatic product and the enzyme have a high action spectrum and a high specific activity as positive aspects. Prebiotic oligosacchartdes are used in nutrition.

Abstract: The invention relates to a novel fructofuranosidase activity for obtaining the prebiotic oligosaccharide 6-kestose. One object of the invention is to characterize a novel transfructosylase activity which is associated with the extracellular invertase of Schwanniomyces occidentalis (specifically the strains selected from the group consisting of ATCC260077, ATCC7410 and ATCC20499) and which can be used to obtain prebiotic oligosaccharides, mainly 6-kestose, which are widely used in the food industry. Another object of the invention relates to the method for obtaining an enzymatic product as well as to the substantially pure enzyme with fructofuranosidase activity.