Abstract: The present invention provides polynucleotides and viral vectors for transfection of a mammalian host cell, preferably lentiviral vectors, encoding at least one CAR (chimeric antigen receptors)and a promoter from the Wiskott-Aldrich syndrome locus, in particular the promoter of SEQ. ID NO 1, operably linked to the CAR in order to drive its expression.

Abstract: A method and system for the hydraulic control of a concrete placing boom (14) comprising a plurality of boom arms (16), wherein hydraulic drive cylinders (18) for the boom arms (16), which are connected to one another in an articulated manner, are controlled by a hydraulic circuit (34), wherein a supply pressure is supplied via a hydraulic pump (32) to the hydraulic circuit (34), and wherein a pressure signal is captured in the drive cylinders (18) by at least one pressure sensor (38) for each cylinder. The maximum pressure in the drive cylinders (18) is determined from the pressure signals and the supply pressure is adjusted by an electronic control unit (36) according to the maximum pressure.

Abstract: A method controls the movement of a boom, wherein the boom is moved by a plurality of hydraulic drives. Each hydraulic drive is fed with a hydraulic medium, the pressure and/or volume flow of which is adjustable. The method predefines a desired direction of movement and a desired speed of a boom tip; predictively calculates a pressure and/or a volume flow required for each of the hydraulic drives that are required for the desired direction of movement and desired speed; subsequently generates a supply pressure depending on the predictively calculated pressures and/or subsequently generating a supply volume flow as a function of the predictively calculated volume flows; and subsequently feeds the hydraulic drives required for the desired direction of movement and desired speed with the hydraulic medium having a respective feed pressure and/or a respective feed volume flow such that the boom tip moves in the desired direction of movement at the desired speed.

Abstract: A method and system for the hydraulic control of a concrete placing boom (14) comprising a plurality of boom arms (16), wherein hydraulic drive cylinders (18) for the boom arms (16), which are connected to one another in an articulated manner, are controlled by a hydraulic circuit (34), wherein a supply pressure is supplied via a hydraulic pump (32) to the hydraulic circuit (34), and wherein a pressure signal is captured in the drive cylinders (18) by at least one pressure sensor (38) for each cylinder. The maximum pressure in the drive cylinders (18) is determined from the pressure signals and the supply pressure is adjusted by an electronic control unit (36) according to the maximum pressure.

Abstract: A battery operated device and method of removing a battery therefrom are described. The battery operated device includes a battery compartment, a battery in the battery compartment, and an electric component powered by the battery. The battery compartment is mounted on a deformable base and includes a top surface which is adapted to be ruptured by deforming the deformable base, thereby enabling removal of the battery from the battery compartment. The method of removing a battery from a battery compartment of a battery operated device includes rupturing a top surface of the battery compartment by deforming a deformable base of the battery compartment, and removing the battery from the battery compartment.

Abstract: A decision tree model is generated from sample data. A visualization system may automatically prune the decision tree model based on characteristics of nodes or branches in the decision tree or based on artifacts associated with model generation. For example, only nodes or questions in the decision tree receiving a largest amount of the sample data may be displayed in the decision tree. The nodes also may be displayed in a manner to more readily identify associated fields or metrics. For example, the nodes may be displayed in different colors and the colors may be associated with different node questions or answers.

Abstract: In the present invention, the inventors have generated a Tet-On all-in-one construct that tightly regulate transgene expression in human stem cells using the original TetR repressor. By using appropriate promoter combinations and shielding the integrative construct with the Is2 insulator, they have constructed the Lent-On-Plus Tet-On system that achieves efficient transgene regulation in human multipotent and pluripotent stem cells. The generation of inducible stem cell lines with the Lent-ON-Plus system did not require selection or cloning, and transgene regulation is maintained after long-term cultured and upon differentiation toward different lineages. The present invention thus offers a solution to the need to improve transgene expression systems in stem cells.

Abstract: The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.

Abstract: A wearable sensing device (100) for sensing one or more physiological signals of a subject, comprising: a sensor unit (200), including a housing, a connection receptacle (210) and electronic circuitry configured for acquiring one or more physiological signals received via the connection receptacle; a patch unit (300), including a connection plug (310) connected via conductive tracks (341) to a plurality of electrodes (342) configured for sensing the one or more physiological signals. The connection plug (310) is configured for being connectable with the connection receptacle (210) such that the one or more physiological signals sensed by the electrodes (342) are transmitted to the electronic circuitry of the sensor unit (200).

Abstract: A system for respiratory monitoring of a subject comprises: a bioimpedance measurement sensor, which is configured for arrangement in relation to the subject for acquiring a bioimpedance signal; a processing unit, which is configured to receive the acquired bioimpedance signal and receive a reference signal, the reference signal representing a respiratory effort of the subject or a respiratory airflow at a time of bioimpedance signal acquirement, the processing unit being further configured to divide the bioimpedance signal into an effort component representing a respiratory effort of the subject and a flow component representing a respiratory airflow of the subject based on the received bioimpedance signal and the received reference signal.

Abstract: The present invention is directed to compositions and methods for delivering a tissue scaffold comprising ultrasound-triggerable agents to an individual.

Abstract: The invention relates to a method for the diagnosis of liver fibrosis, comprising detecting the level of expression of the gene GATA-4, or the quantity of the protein GATA-4, in the isolated biological sample of liver tissue. The invention also relates to a diagnosis kit.

Abstract: An electronic system for bioimpedance signal acquisition, comprises: a current signal injection module configured for generating a current signal to be applied to a subject; a bioimpedance signal measurement module configured for measuring a bioimpedance signal based on a voltage generated by the current signal; a data quality detection module configured for detecting an AC or a DC level of the measured bioimpedance signal and detecting whether the AC or DC level is within or outside an AC reference value range and a DC reference value range, respectively; and a signal adaptation module configured for modifying at least one parameter of the current signal injection module or the bioimpedance signal measurement module based on said detection of the AC or DC level in relation to the AC reference value range and the DC reference value range, respectively.

Abstract: The present invention solves the problem of providing more efficient barrier insulators to avoid vector silencing and to increase expression in the setting of gene transfer vectors, more particularly in the setting of gene transfer retroviral vectors. In this sense, the authors of the present invention have developed an improved insulator element, namely element IS2, which comprises the following combination of nucleic acid molecules, namely nucleic acid molecule HS4-650 bp as shown in SEQ ID No 2 and a synthetic S/MAR nucleic acid molecule containing 5 M/SARs recognition signatures (MRS) as shown in SEQ ID no 1.

Abstract: The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.

Abstract: The present invention falls into the context of green chemistry and generically relates to a fertilizing and phytoprotective formulation and, in particular embodiment, to a film forming formulation that induces resistance to plants. The respective formulation, when applied to plants and/or fruits, results in the formation of a film on the surface of the material, which has a characteristic of photoprotection against UV-B and UV-C radiations, resistance kept in water, even after high hygroscopicity, greater stability at high ambient temperatures, formation of desired porosity and surface homogeneity.

Abstract: The invention relates to expression systems useful for regulated expression of a gene of interest based on the constitutive expression of the original TetR repressor and the expression of the polynucleotide driven by a constitutive promoter operably linked to an operator sequence for a tetracycline operator sequence. The system can be provided as two different polynucleotides or as an all-in-one vector. The invention also relates to vectors, host cells and viral particles according to the invention as well as to the uses thereof for in vitro and in vivo production of products of interest or for therapy.

Abstract: The present invention relates to a novel glycoprotein-A repetitions predominant protein (GARP) surface marker for mesenchymal stem cells, specifically activated mesenchymal stem cells, and to a method for the isolation and/or identification of mesenchymal stem cells based on the detection of the glycoprotein-A repetitions predominant protein (GARP) surface marker. The invention also relates to mesenchymal stem cells expressing said marker and to the uses thereof.

Abstract: A battery operated device and method of removing a battery therefrom are described. The battery operated device includes a battery compartment, a battery in the battery compartment, and an electric component powered by the battery. The battery compartment is mounted on a deformable base and includes a top surface which is adapted to be ruptured by deforming the deformable base, thereby enabling removal of the battery from the battery compartment. The method of removing a battery from a battery compartment of a battery operated device includes rupturing a top surface of the battery compartment by deforming a deformable base of the battery compartment, and removing the battery from the battery compartment.

Abstract: The present invention is based on the surprising discovery that the overexpression of the SCL gene, of nucleotide sequence SEQ ID NO: 1, increases the production of megakaryocytes and platelets from stem cells. Likewise, it has been proven that this increase also occurs using PSCs which, unlike CD34+ HSC cells which have a limited expansion capacity, are characterized by an unlimited growth capacity, and can result in the unlimited production of platelets for the use thereof in clinics.