Abstract: In a method for manufacturing a three dimensional object by extrusion based modelling, tracks of modelling material are deposited in slices by a printhead connected to a three dimensional positioning system. In each slice a plurality of first tracks of three-dimensional modelling material is deposited by the printhead under a first pressure, wherein the first tracks are spaced apart so as to leave a gap between them. A second track of three-dimensional modelling material is deposited by the printhead in each of the gaps between the deposited first tracks under a second pressure, which is higher than the first pressure, such that the gaps are filled entirely. A monolithic body is formed by depositing the modelling material slice by slice.

Abstract: The present disclosure relates to genetically modified conditionally immortalized cells and cell lines, provided with a vector comprising a promoter sequence, at least one immortalization gene operably linked to the promoter sequence, a gene coding for an inducible regulator operably linked to the promoter sequence and a response element for the inducible regulator. The present disclosure further relates to methods for their preparation, and to immortalization and differentiation of such cells.

Abstract: A plastic-based composite product comprising a plastic mass in which particles are homogeneously embedded, which particles have tensile strength in a first particle direction, the product having a chosen principal product direction, wherein the particles comprise: small particles being elongated plates of fibres and having a random orientation and a length of 0.2-2 mm; and large particles dominantly orientated such that their first particle direction is in the chosen principal product direction, the large particles having a length in the first particle direction of about 2-6 mm.

Abstract: A building construction includes a slab-like floor layer having an upper surface defining a habitable area and at least one horizontal groove opening into the lower surface of the layer, a plurality of duct-like parts being supported on the upper surface of the floor layer in accord with an arbitrarily selected room layout, utility lines for the habitable area being disposed in the horizontal groove in the lower surface of the layer and also being disposed in the duct-like parts above the upper surface, and partition walls being disposed atop the duct-like parts to form rooms.

Abstract: A method of building construction which involves the steps of forming a building framework that includes a slab-like floor layer having an upper surface defining a habitable area and at least one horizontal groove opening into its lower surface, providing a pattern of duct-like parts on the floor layer in accord with an arbitrarily selected room layout, providing utility lines in the horizontal groove below the upper surface and in the duct-like parts above the upper surface, and providing partition walls atop the duct-like parts to form rooms.