Abstract: The present disclosure relates to a method for detecting a mutation in a microsatellite sequence locus of a target fragment from a DNA sample, comprising a step of subjecting said DNA sample to a digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers suitable for amplifying said target fragment of the DNA sample including said microsatellite sequence; a first MS oligonucleotide (MS) hydrolysis probe, labeled with a first fluorophore, wherein said first MS oligonucleotide probe is complementary to a wild-type sequence including the microsatellite sequence; a second oligonucleotide reference (REF) hydrolysis probe, labeled with a second fluorophore, wherein said second oligonucleotide REF probe is complementary to a wild-type sequence of said target DNA fragment which does not include said microsatellite sequence.

Abstract: The present invention relates to a method for identifying a cancer patient presenting microsatellite unstable tumor who is likely to benefit from immunotherapy comprising detecting a mutation within a microsatellite sequence by subjecting said sample to a digital polymerase chain reaction. The present invention also encompasses an immune checkpoint inhibitor for use in the treatment of a cancer in a patient who is previously identified as likely benefit from an immunotherapy and a method for evaluating the therapeutic response of an immune checkpoint inhibitor in a patient having a cancer.

Abstract: The present disclosure relates to a method for detecting a mutation in a microsatellite sequence locus of a target fragment from a DNA sample, comprising a step of subjecting said DNA sample to a digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers suitable for amplifying said target fragment of the DNA sample including said microsatellite sequence; a first MS oligonucleotide (MS) hydrolysis probe, labeled with a first fluorophore, wherein said first MS oligonucleotide probe is complementary to a wild-type sequence including the microsatellite sequence; a second oligonucleotide reference (REF) hydrolysis probe, labeled with a second fluorophore, wherein said second oligonucleotide REF probe is complementary to a wild-type sequence of said target DNA fragment which does not include said microsatellite sequence.

Abstract: The invention relates to a method for detecting a mutation in a microsatellite sequence locus of a target fragment from a DNA sample, comprising subjecting said DNA sample to a digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers for amplifying said target fragment of the DNA sample including said microsatellite sequence; a first MS oligonucleotide (MS) hydrolysis probe, labeled with a first fluorophore, wherein said first MS oligonucleotide probe is complementary to a wild-type sequence including the microsatellite sequence; and a second oligonucleotide reference (REF) hydrolysis probe, labeled with a second fluorophore, wherein said second oligonucleotide REF probe is complementary to a wild-type sequence of said target DNA fragment which does not include said microsatellite sequence. The invention also encompasses methods for the diagnosis and prognosis of cancer and a method for determining the efficacy of a cancer treatment.

Abstract: Disclosed is a portable hot air generator apparatus which includes a handle and a body forming a Venturi and, furthermore, a damping system between the handle and the body.

Abstract: The present invention relates to an in vitro method for identifying and/or characterizing one or more mutations in a hotspot mutation sequence of at least one ESR1 target fragment from a DNA sample, said method comprising subjecting the DNA sample to a drop-off digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers suitable for amplifying an ESR1 target fragment; an oligonucleotide reference (REF) hydrolysis probe, labeled with a fluorophore, wherein said REF oligonucleotide probe is complementary to a wild-type sequence of the target fragment located outside of the hotspot mutation sequence; an oligonucleotide hotspot (HOTSPOT) hydrolysis probe, labeled with another fluorophore, wherein said oligonucleotide HOTSPOT probe is complementary to a wild-type sequence of the hotspot mutation sequence of the target DNA fragment.

Abstract: The invention relates to a method for detecting a mutation in a microsatellite sequence locus of a target fragment from a DNA sample, comprising subjecting said DNA sample to a digital polymerase chain reaction (PCR) in the presence of a PCR solution comprising: a pair of primers for amplifying said target fragment of the DNA sample including said microsatellite sequence; a first MS oligonucleotide (MS) hydrolysis probe, labeled with a first fluorophore, wherein said first MS oligonucleotide probe is complementary to a wild-type sequence including the microsatellite sequence; a second oligonucleotide reference (REF) hydrolysis probe, labeled with a second fluorophore, wherein said second oligonucleotide REF probe is complementary to a wild-type sequence of said target DNA fragment which does not include said microsatellite sequence. The invention also encompasses methods for the diagnosis and prognosis of cancer and a method for determining the efficacy of a cancer treatment.

Abstract: A heat-shrink gun in which an ignition voltage of the gaseous mixture is generated by a piezoelectric lighter (12), under the action of a trigger (8) when it is moved from a waiting position (8A) to a lighting position, characterized in that the trigger can also assume a loading position (8B), in which it free access (DC) to a working housing for the lighter.

Abstract: The invention relates to an apparatus comprising an injection device (1) for producing a flow of a high-speed combustible gas mixture, and a burner head (2) in which said flow is injected through a tabular extender (70) connecting said injection device (1) to said head (2). The extender has a maximal length higher than 600 mm and includes at the gravity center of the device assembly, means for suspension to a portion of the operator's body.

Abstract: A hot air generating device of the type including an injection device (2) capable of producing a flow of a combustible gas mixture at a high speed and a burner head (3) in which the flow is ignited via a spark plug (6) positioned near the burner (3) and electrically connected to a lighter (12), preferably a piezoelectric lighter, including an autotransformer mounted in parallel between the lighter and the spark plug. Such a device is particularly advantageous to facilitate the lighting of the mixture, in particular when the device is provided with a riser between the lighter and the spark plug.

Abstract: A heat-shrink gun in which an ignition voltage of the gaseous mixture is generated by a piezoelectric lighter (12), under the action of a trigger (8) when it is moved from a waiting position (8A) to a lighting position, characterized in that the trigger can also assume a loading position (8B), in which it free access (DC) to a working housing for the lighter.

Abstract: The invention concerns a method for verifying, prior to fabrication, the proper operation of integrated circuit electronic systems using analog signals. It comprises the following steps: identifying (22) the noise-sensitive circuits, setting an acceptable sensitivity template for these noise-sensitive circuits, identifying (34) the noise-generating circuits, modeling the noise, determining (50) the function for transferring noise to the sensitive circuits, and comparing (58) the level of noise reaching the sensitive circuits to an acceptable sensitivity threshold template for the sensitive circuits.

Abstract: Method for modelling the noise injected into an electronic system. The invention relates to a method of modelling the noise injected into a mixed system (1) of digital and analogue, and/or radio-frequency type. In the invention, the injection of noise in the system (1) is modelled by macro-models of digital cells (8, 8.1-8.N) which model, in particular, noise related to the switching of the digital cells (C1-CN), and by models of lines (L1-LN) modelling, in particular, the noise resulting from a change of state of the signals transported over the lines.

Abstract: The invention relates to an apparatus comprising an injection device (1) for producing a flow of a high-speed combustible gas mixture, and a burner head (2) in which said flow is injected through a tabular extender (70) connecting said injection device (1) to said head (2). The extender has a maximal length higher than 600 mm and includes at the gravity centre of the device assembly, means for suspension to a portion of the operator's body.

Abstract: The present invention is a method for modeling the switching activity of a digital circuit, this digital circuit comprising cells linked together by interconnections, these cells switching at an instant at which at least one of their inputs changes state, successive switchings of the cells of the circuits occurring during a clock period Tclk of this circuit, the clock period includes the following steps: calculating based on an adapted statistical model the number of cells liable to switch over each time interval [tk, tk+1] of the clock period, and allocating switching instants to the different cells of the digital circuit based on the knowledge of the number of cells liable to switch over the time intervals [tk; tk+1]. The present invention is further a production method.

Abstract: The invention concerns a method for verifying, prior to fabrication, the proper operation of integrated circuit electronic systems using analog signals. It comprises the following steps: identifying (22) the noise-sensitive circuits, setting an acceptable sensitivity template for these noise-sensitive circuits, identifying (34) the noise-generating circuits, modeling the noise, determining (50) the function for transferring noise to the sensitive circuits, and comparing (58) the level of noise reaching the sensitive circuits to an acceptable sensitivity threshold template for the sensitive circuits.

Abstract: Titanium-based metal parts (1, 3) are soldered by using as a filler metal (7) an aluminium alloy containing magnesium and virtually no silicon. Application to the bonding of blades (3) to the inner shroud (1) of an aeronautical gas turbine engine compressor nozzle ring.