Abstract: This disclosure provides a preserving composition comprising a cholesterol: carrier complex, optionally a cholesterol:cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.

Abstract: A method of transforming a mitochondrion of a plant cell to express a nitrogenase enzyme includes exposing the plant cell to a mitochondrial-targeting nanocarrier polypeptide and one or more nucleic acids encoding the nitrogenase enzyme. The one or more genes encoding the nitrogenase enzyme can be one or more Klebsiella nif genes. The method can be used to generate plants which have the capability of fixing atmospheric elemental nitrogen.

Abstract: A heat exchanger assembly has a frame including: a plurality of legs; first and second lower transversal members extending perpendicular to and interconnecting the legs; a plurality of upstanding members extending upwardly from respective ones of the legs, a lower end of each upstanding member being connected to a corresponding leg at a junction therebetween; an upper transversal member interconnecting upper ends of the upstanding members; and an upper frame assembly. The frame components are weldlessly connected to one another. First and second heat exchanger panels exchange heat with air pulled into the heat exchanger assembly and are disposed in a V-configuration. An upper end of each heat exchanger panel is connected to upper retaining members of the upper frame assembly. A fan pulls air into the enclosed space of the heat exchanger assembly via at least one of the heat exchanger panels.

Abstract: A heat exchanger assembly includes a frame; first and second heat exchanger panels configured for exchanging heat with air pulled into the heat exchanger assembly and disposed in a V-configuration; a fan for pulling air into an enclosed space of the heat exchanger assembly; a plurality of lateral enclosing panels and at least one middle enclosing panel. The at least one middle enclosing panel extends perpendicular to the lateral enclosing panels and is disposed between the heat exchanger panels. A U-shaped lower end of the at least one middle enclosing panel includes first and second wall portions opposite one another. The lower ends of the heat exchanger panels abut the wall portions such that the wall portions are deflected relative to one another by forces exerted thereon by the lower ends causing a first and second seals to form between the wall portions and the heat exchanger panels.

Abstract: This disclosure provides a preserving composition comprising a cholesterol: carrier complex, optionally a cholesterol: cyclodextrin complex (CC complex) and/or a cell permeable antioxidant peptide and a biological buffer and optionally a cryprotectant, wherein the preserving composition is optionally substantially free of animal phospholipid, animal protein and/or animal lipoprotein. The disclosure also provides methods for the use of the preserving composition in cryopreservation of semen or sperm cells. Also provided is a kit comprising the preserving composition having a CC complex, a biological buffer, a cryoprotectant, a carbohydrate, a pH stabilizer, an antibiotic or antibiotic cocktail and/or a cell permeable anti-oxidant peptide.

Abstract: A method of transforming a mitochondrion of a plant cell to express a nitrogenase enzyme includes exposing the plant cell to a mitochondrial-targeting nanocarrier polypeptide and one or more nucleic acids encoding the nitrogenase enzyme. The one or more genes encoding the nitrogenase enzyme can be one or more Klebsiella nif genes. The method can be used to generate plants which have the capability of fixing atmospheric elemental nitrogen.

Abstract: A heat exchanger assembly has a frame including: a plurality of legs; first and second lower transversal members extending perpendicular to and interconnecting the legs; a plurality of upstanding members extending upwardly from respective ones of the legs, a lower end of each upstanding member being connected to a corresponding leg at a junction therebetween; an upper transversal member interconnecting upper ends of the upstanding members; and an upper frame assembly. The frame components are weldlessly connected to one another. First and second heat exchanger panels exchange heat with air pulled into the heat exchanger assembly and are disposed in a V-configuration. An upper end of each heat exchanger panel is connected to upper retaining members of the upper frame assembly. A fan pulls air into the enclosed space of the heat exchanger assembly via at least one of the heat exchanger panels.

Abstract: A heat exchanger assembly includes a frame; first and second heat exchanger panels configured for exchanging heat with air pulled into the heat exchanger assembly and disposed in a V-configuration; a fan for pulling air into an enclosed space of the heat exchanger assembly; a plurality of lateral enclosing panels and at least one middle enclosing panel. The at least one middle enclosing panel extends perpendicular to the lateral enclosing panels and is disposed between the heat exchanger panels. A U-shaped lower end of the at least one middle enclosing panel includes first and second wall portions opposite one another. The lower ends of the heat exchanger panels abut the wall portions such that the wall portions are deflected relative to one another by forces exerted thereon by the lower ends causing a first and second seals to form between the wall portions and the heat exchanger panels.

Abstract: Compositions including a nanocomplex of a polynucleotide with a cell-penetrating peptide and a quaternary phosphonium salt, such as tetrabutylphosphonium bromide (TBPB), are useful for delivering polynucleotides such as DNA or RNA into cells.

Abstract: The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.

Abstract: The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.

Abstract: Provided are organelle targeting nanocarriers, including peptides, which act to deliver biological molecules such as nucleic acids to non-nuclear organelles such as mitochondria and chloroplasts. Also provided are methods for genetic transformation of non-nuclear organelles using such nanocarriers.

Abstract: The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.

Abstract: A device for mixing powder of the like with a liquid, the device including both a dispersion tube having its bottom portion open and designed to be in the liquid and having a delivery orifice for powder or the like in its top portion, and a mixer located in the dispersion tube and including a first rotary stirrer disposed in the vicinity of the bottom end of the dispersion tube and suitable for creating a first downward stream in the dispersion tube. The mixer further includes a second rotary stirrer disposed between the delivery orifice for powder or the like and the first rotary stirrer, and suitable for creating a second downward stream in the dispersion tube.

Abstract: The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.

Abstract: A DNA/protein nano-complex for transfecting eukaryotic cells, wherein the nano-complex comprises an expressible nucleotide molecule complexed with a VirD2 protein molecule, and a RecA protein molecule complexed with the nucleotide molecule/VirD2 protein molecule complex. The nucleotide molecule/VirD2 protein molecule/RecA protein molecule complexed may be further complexed to a cell penetrating peptide. The DNA/protein nano-complex can be used for delivering the expressible nucleotide molecule to target plant cells, in particular, monocot plant cells exemplified by wheat cells, corn cells, triticale cells, barley cells, and rye cells.

Abstract: The present invention provides a novel method for the transduction and/or transfection of plant cells. Cell-penetrating peptides (CPPs) have been successfully employed as nanocarriers to deliver proteins and oligonucleotides to single plant cell microspores as well as multi-cellular zygotic embryos. The efficiency of CPP internalization and further delivery of a macromolecular cargo comprising a protein and/or an oligonucleotide can be enhanced by permeabilization of the zygotic embryos.

Abstract: A device for mixing powder or the like with a liquid, the device comprising both a dispersion tube having its bottom portion open and designed to be in the liquid and having a delivery orifice for powder or the like in its top portion, and mixer means located in the dispersion tube and comprising a first rotary stirrer disposed in the vicinity of the bottom end of the dispersion tube and suitable for creating a first downward stream in said dispersion tube. The mixer means further comprise a second rotary stirrer disposed between the delivery orifice for powder or the like and the first rotary stirrer, and suitable for creating a second downward stream in said dispersion tube.

Abstract: The growing controversy over GM/novel-trait crops and food products that possess novel industrial or nutritional traits, has resulted in an increased need for technologies that allow the rapid and reliable identification of GM/novel-trait crops so that they can be identified, and segregated if necessary, at the various stages of production and processing. A visual seed identity system is described that permits the identification and tracing of each individual seed through the upstream value chain, from field increase to the processing plant. These traits are genetically controlled, transmitted to progeny by either pollen or ovule and expressed in developing seed, showing a xenia effect. That novelty of this proposed IP system will facilitate early mitigation measures if out-crossing or admixtures occur and exceed a threshold limit set by regulatory agencies.