Abstract: A method for producing proteins of interest from secondary root emergences which appear on the hairy roots of a plant belonging to the Brassicaceae family, in a liquid medium containing at least one auxin, comprises the use of a strain of Rhizobium comprising rol genes.

Abstract: Disclosed is a method for producing proteins of interest from secondary root emergences which appear on the hairy roots of a plant belonging to the Brassicaceae family, in a liquid medium containing at least one auxin.

Abstract: The invention relates to a method for producing recombinant proteins from transgenic hairy roots, in particular transgenic hairy roots obtained by transforming plants belonging to the Brassicaceae family with Agrobacterium rhizogenes and/or with Agrobacterium tumefaciens.

Abstract: Plant cells are transformed by:(i) transforming a plant cell whose growth is sensitive to inhibition by a sulfonamide or a salt thereof with a chimaeric gene comprising (a) a plant promoter, (b) a sulfonamide resistance gene having a sequence encoding a transit peptide fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence;(ii) selecting a transformed plant cell whose growth is resistant to inhibition by a sulfonamide or salt thereof;(iii) optionally, regenerating from the transformed plant cell a genetically transformed plant which exhibit the said resistance;(iv) optionally, obtaining seed from the regenerated plant; and(v) optionally, propagating plants from the seed.The growth of weeds can be controlled at a locus where a transgenic plant obtained as above is being cultivated by applying to the locus an effective amount of a herbicide, such as asulam, which acts by inhibiting dihydropteroate synthase.