Abstract: The present invention relates to a sulfolobus expression vector comprising: (a) sulfolobus origin of replication; (b) the genes encoding the structural proteins and the site-specific integrase of SSV1, SSV2 or pSSVx, operatively linked to expression control sequences and a packaging signal; (c) one or more selectable marker gene(s), operatively linked to sulfolobus expression control sequences; and (d) a sulfolobus promoter followed 3? by a restriction enzyme recognition site or a multiple cloning site for insertion of a gene of interest and optionally a 3? regulatory element. Moreover, the present invention relates to a shuttle vector comprising the sequences of the expression vector of the invention and additional sequences for propagation and selection in E. coli, wherein the additional sequences comprise (a) an E. coli on of replication; and (b) a marker for selection in E. coli.

Abstract: We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds.

Abstract: We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds.

Abstract: The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another. The DNA of the two proteases, was obtained from soil samples, and C-terminally deleted, producing proteolytically active fragments thereof (SEQ ID NO: 5 and 8). The invention also provides all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.

Abstract: The present application relates to two novel proteases (SEQ ID NO: 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and C-terminally deleted, likewise proteolytically active fragments thereof (SEQ ID NO: 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO: 4 or to 87.5% to SEQ ID NO: 7, and those which can be summarized under a consensus sequence (SEQ ID NO: 9) derived from SEQ ID NO: 4 and 7. Furthermore, it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO: 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.

Abstract: An alkaline protease, the DNA thereof having been taken from a bottom sample, alkaline proteases that are at least 40% identical, and nucleic acids with an identity at least 50% homologous with the associated nucleic acids. The invention also relates to the fragment of said protease comprising the amino acid positions 108 to 325, in addition to the gene fragment coding therefor, and alkaline proteases that are at least 60% identical or nucleic acids that are at least 70% identical. Furthermore, the invention defines technical possibilities of use for said proteases, especially use thereof in washing and cleaning products.

Abstract: The present application relates to two novel alkaline proteases (SEQ ID NO. 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and which were C-terminally deleted. The present application also provides proteolytically active fragments thereof (SEQ ID NO. 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO. 4 or to 87.5% to SEQ ID NO. 7, and those which can be summarized under a consensus sequence (SEQ ID NO. 9) derived from SEQ ID NO. 4 and 7. Furthermore, the present application relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO. 3 and 6) or the fragments concerned. Furthermore, the present application relates to use for these proteases and especially describes their use in detergents and cleaners.

Abstract: The invention relates to biocatalysts showing alcohol dehydrogenase activity obtainable from Rhodococcus ruber, their preparation, their use in the oxidation of secondary alcohols and/or the reduction of ketones, as well as nucleic acids coding for these alcohol dehydrogenases and microorganisms transformed with nucleic acids coding for these biocatalysts and their use for producing the biocatalyst or oxidizing secondary alcohols and/or reducing ketones.

Abstract: The present application relates to two novel alkaline proteases (SEQ ID NO. 4 and 7) which are similar to one another, whose DNA was obtained from soil samples, and C-terminally deleted, likewise proteolytically active fragments thereof (SEQ ID NO. 5 and 8), all alkaline proteases similar at least to 90% to SEQ ID NO. 4 or to 87.5% to SEQ ID NO. 7, and those which can be summarized under a consensus sequence (SEQ ID NO. 9) derived from SEQ ID NO. 4 and 7. Furthermore it relates to all nucleic acids which have a homology of at least 85% identity to the associated nucleic acids (SEQ ID NO. 3 and 6) or the fragments concerned. Furthermore, it defines technical possibilities of use for these proteases and especially describes their use in detergents and cleaners.

Abstract: The invention relates to a novel alkaline protease, the DNA thereof having been taken from a bottom sample, to all alkaline proteases that are at least 40% identical, and to all nucleic acids with an identity at least 50% homologous with the associated nucleic acids. The invention also relates to the fragment of said protease, comprising the amino acid positions 108 to 325, in addition to the gene fragment coding therefor, and to all alkaline proteases that are at least 60% identical, or nucleic acids that are at least 70% identical. Furthermore, the invention defines technical possibilities of use for said proteases and especially describes the use thereof in washing and cleaning products.

Abstract: The invention relates to biocatalysts showing alcohol dehydrogenase activity obtainable from Rhodococcus ruber, their preparation, their use in the oxidation of secondary alcohols and/or the reduction of ketones, as well as nucleic acids coding for these alcohol dehydrogenases and microorganisms transformed with nucleic acids coding for these biocatalysts and their use for producing the biocatalyst or oxidizing secondary alcohols and/or reducing ketones.

Abstract: Safe transportation holder and package for explosive devices such as blasg caps. Each cap is contained in an internal cavity in a holder, and surrounded by radially-spaced, elastomeric walls. The holders are arrayed in a container, and absorb the energy released by accidental detonation of one cap to prevent sympathetic detonation of others in the package.

Abstract: We have identified by molecular cloning a protease which originates from the larvae of Lucilia sericata and which was termed debrilase due to its activities useful for debridement of wounds.