Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

Abstract: The invention relates to a profile spacing element for mounting of a second glass (2) in a window frame (1) said spacing element serves as a spacer between the glasses. The space between the glasses and the spacing element is sealed. The spacing element (6,15) preferably consists of a non-rigid material which is provided with a diffusion tight covering (9). The covering extends along the entire length of the spacing element and covers its sides (7,8,10) which face towards the glasses (2,5) and the window frame (1). The covering perfects the sealing contact between the spacing element and the glasses and window frame. The diffusion tight material (9) consists of an elastic or resilient material. Further the spacing element preferably is formed as a hollow profile filled with a drying agent and having longitudinally extending deformation notches (11,12) making it possible to bend the spacing element at the corner areas of the window frame without pressing the glasses apart from each other.