Abstract: Among other things, the present disclosure provides designed oligonucleotides, compositions, and methods thereof. In some embodiments, provided oligonucleotide compositions provide improved single-stranded RNA interference and/or RNase H-mediated knockdown. Among other things, the present disclosure encompasses the recognition that structural elements of oligonucleotides, such as base sequence, chemical modifications (e.g., modifications of sugar, base, and/or internucleotidic linkages) or patterns thereof, conjugation with additional chemical moieties, and/or stereochemistry [e.g., stereochemistry of backbone chiral centers (chiral internucleotidic linkages)], and/or patterns thereof, can have significant impact on oligonucleotide properties and activities, e.g., RNA interference (RNAi) activity, stability, delivery, etc.

Abstract: Among other things, the present disclosure relates to designed oligonucleotides, compositions, and methods thereof. In some embodiments, provided oligonucleotide compositions provide altered splicing of a transcript. In some embodiments, provided oligonucleotide compositions have low toxicity. In some embodiments, provided oligonucleotide compositions provide improved protein binding profiles. In some embodiments, provided oligonucleotide compositions have improved delivery. In some embodiments, provided oligonucleotide compositions have improved uptake. In some embodiments, the present disclosure provides methods for treatment of diseases using provided oligonucleotide compositions.

Abstract: Among other things, the present disclosure provides designed APOC3 oligonucleotides, compositions, and methods thereof. In some embodiments, provided oligonucleotide compositions provide improved single-stranded RNA interference and/or RNase H-mediated knockdown. Among other things, the present disclosure encompasses the recognition that structural elements of oligonucleotides, such as base sequence, chemical modifications (e.g., modifications of sugar, base, and/or internucleotidic linkages) or patterns thereof, conjugation with additional chemical moieties, and/or stereochemistry [e.g., stereochemistry of backbone chiral centers (chiral internucleotidic linkages)], and/or patterns thereof, can have significant impact on oligonucleotide properties and activities, e.g., RNA interference (RNAi) activity, stability, delivery, etc.

Abstract: The present invention provides a novel insecticide formulation that not only demonstrates strong insecticidal and residual activity against a variety of insects, but also exhibits low mammalian toxicity. In certain embodiments, the present invention relates to the use of pesticidal compounds selected from beta damascone, CYCLEMONE A™, melafleur, and derivatives thereof for killing arthropod pests including, but not limited to, mosquitoes and flies. Specifically exemplified herein is the use of the compounds of the present invention to control three mosquitoes (Aedes aegypti, Ae. albopictus, and Anopheles quadrimaculatus), the house fly (Musca domestica), the stable fly (Stomoxys calcitrans) and the sand fly (Lutzomyia shannoni).

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on a switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format. Optical and/or fluidic switching forces are used alone or in combination to effect switching.

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on an optical switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format.

Abstract: The present invention provides a novel insecticide formulation that not only demonstrates strong insecticidal and residual activity against a variety of insects, but also exhibits low mammalian toxicity.

Abstract: A time limited restraint device. After a predetermined time, a cutter of the time limited restraint device cuts a restraint.

Abstract: A method of determining a particle size distribution in a wellbore fluid including collecting a volume of mud from a vibratory separator, sampling a volume of the collected mud, and testing the volume of collected mud with a test kit to determine the concentration of a sized additive in the mud is disclosed. A system for determining particle size distribution of a fluid, the system including a vibratory separator, a meter configured to receive a separated material from the vibratory separator, a counter configured to count the number of loads collected by the meter, a test kit including a sieve and a measuring tube, and a centrifuged configured to receive the measuring tube is also disclosed.

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on an optical switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format.

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on an optical switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format.

Abstract: A time limited restraint device. After a predetermined time, a cutter of the time limited restraint device cuts a restraint.

Abstract: A process for the recovery of drilling fluid additives, including industrial carbon, calcium carbonate, natural and synthetic fibers, and other materials from a mixture. The mixture may include drilling fluids, drilled solids, and drilling fluid additives from a mud system. The process may include: separating at least a portion of the drilled solids from the mixture to form a first effluent and a drilled solids fraction; separating at least a portion of the drilling fluid additives from the first effluent to form a second effluent and a recovered additives fraction; and recycling at least a portion of the recovered additives fraction to the mud system. The drilling fluid additives may have a specific gravity greater than 1.4, and may include particles having an average size greater than 2 microns.

Abstract: Apparatus and Methods are provided for a microfabricated fluorescence activated cell sorter based on a switch for rapid, active control of cell routing through a microfluidic channel network. This sorter enables low-stress, highly efficient sorting of populations of small numbers of cells (i.e., 1000-100,000 cells). The invention includes packaging of the microfluidic channel network in a self-contained plastic cartridge that enables microfluidic channel network to macro-scale instrument interconnect, in a sterile, disposable format. Optical and/or fluidic switching forces are used alone or in combination to effect switching.

Abstract: A process for the recovery of industrial carbon material from a mixture. The mixture may include drilling fluids, drilled solids, and industrial carbon from a mud system. The process may include: separating at least a portion of the drilled solids from the mixture to form a first effluent and a drilled solids fraction; separating at least a portion of the industrial carbon from the first effluent to form a second effluent and a recovered industrial carbon fraction; and recycling at least a portion of the recovered industrial carbon to the mud system.

Abstract: A process for the recovery of drilling fluid additives, including industrial carbon, calcium carbonate, natural and synthetic fibers, and other materials from a mixture. The mixture may include drilling fluids, drilled solids, and drilling fluid additives from a mud system. The process may include: separating at least a portion of the drilled solids from the mixture to form a first effluent and a drilled solids fraction; separating at least a portion of the drilling fluid additives from the first effluent to form a second effluent and a recovered additives fraction; and recycling at least a portion of the recovered additives fraction to the mud system. The drilling fluid additives may have a specific gravity greater than 1.4, and may include particles having an average size greater than 2 microns.

Abstract: A process for the recovery of industrial carbon material from a mixture. The mixture may include drilling fluids, drilled solids, and industrial carbon from a mud system. The process may include: separating at least a portion of the drilled solids from the mixture to form a first effluent and a drilled solids fraction; separating at least a portion of the industrial carbon from the first effluent to form a second effluent and a recovered industrial carbon fraction; and recycling at least a portion of the recovered industrial carbon to the mud system.

Abstract: A modular scaffolding assembly in which the platform may move up or down in a continuous motion. A platform carriage is engaged with a tower by spring loaded friction blocks and climbs or descends the tower along a pair of stepped racks. Platform modules may be removably connected to the platform carriage.