Abstract: A method and apparatus for counting cells in image regions of a phase image of a cell culture in a cell culture vessel, comprising finding the local maximum of cell size of each image region, creating a local threshold value in each cell-sized image region, creating a mask using the threshold value which separates each cell to segment the cells into cell phase regions, creating a contour around the edge of each segmented cell phase region to define a cell outline, creating lists of the cell outlines, wherein each cell has its own outline list of points and shape parameters; and counting the number of cells using the lists.

Abstract: A method and apparatus for quantitative phase imaging a cell culture in a cell culture vessel has an imager for providing a plurality of z-stack brightfield images of at least a portion of the cell culture in the cell culture vessel, each image corresponding to a different focal distance relative to the cell culture and each image comprising an array of pixels and at least one processor configured to receive the plurality of z-stack brightfield images.

Abstract: A method and apparatus for counting cells in image regions of a phase image of a cell culture in a cell culture vessel, has an imager for imaging a cell culture in a cell culture vessel and at least on processor for providing a phase image of the cell culture and configured to: find the local maximum of each source image region, wherein the size of each source image region is defined by an approximated diameter by creating an intermediate image using an elliptical structuring element mask having a deleted central pixel and performing a morphological operation of dilation on the source image; for each source image region create a new mask image as a logical ‘LARGER THAN’ of the intermediate image with the source image resulting in zero everywhere except at a single pixel dot located at a pixel location wherein the source image has a local maximum value of a local region and wherein the size of the local region is determined by the size of the elliptical structuring element mask resulting in the mask image havi

Abstract: Methods for the image processing od cells are disclosed for the purpose of segmenting cell colonies for further processing to determine confluence, counting and morphology.

Abstract: Methods for the image processing of cells are disclosed for the purpose of segmenting cell colonies for further processing to determine confluence, counting and morphology.

Abstract: Method and apparatus for displaying cultured cell wherein the image can be smoothly moved into and out of focus and can be zoomed in and out and panned.

Abstract: A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

Abstract: A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

Abstract: A method and apparatus for measuring cells and cell confluence includes an imager for imaging a z-stack of bright field images and a processor for selecting a best focus bright field image at a z position of f microns in the z-stack of images. In the manner described herein, the processor selects a bright image n microns lower in the z-stack than the best focus bright field image and a dark image m microns higher in the stack than the best focus bright field image. The processor applies edge detection to the dark image to obtain an edge image and subtracts the edge image from the dark image and the processor applies an adaptive threshold to the bright image to create a mask and uses the adaptive thresholded image as seeds to evaluate the edge image to find cell candidates.

Abstract: A method and system comprise taking a series of time spaced images of a cell culture having pathogens therein creating plaques, applying a stain to the cell culture, taking an image of the stained plaques, using the image of the stained culture to build a model of the plaques in earlier pre-stain images of the culture and displaying the pre-stain images and identifying the plaques therein based upon the model.

Abstract: Method and apparatus for performing a plaque detection by using above focus and below focus images. Plaque is also detected by a method and apparatus using test and training data captured on an imaging system, building a new model for a specific virus/cell/protocol type to detect plaques, using the models in runtime systems to detect plaques and augmenting the models based on automatically calculated false positive and false negative counts and percentages taken from test runs and/or runtime data.

Abstract: A plaque detection method and apparatus wherein at least one processor is programmed to receive above focus images to detect the presence of live cells without detecting the lysed cell materials, receive below focus images wherein virtual dark regions exist which are similar to cell shadows as seeds in a segmentation process and use contours around each resulting shape to obtain a subset that are more likely to be part of the cell population to define a cell map. A distance map is created in which each pixel value is the distance of that pixel from the nearest pixel of the cell map and the distance map is thresholded to create a first image of the places which are relatively far from the cells a second image with a smaller distance threshold to get an image that mimics the edges of the cells.

Abstract: Methods for the image processing od cells are disclosed for the purpose of segmenting cell colonies for further processing to determine confluence, counting and morphology.

Abstract: Method and apparatus for displaying cultured cell wherein the image can be smoothly moved into and out of focus and can be zoomed in and out and panned.

Abstract: Systems and methods for dynamic computer aided innovation via multidimensional complementary difference recommendation and exploration are disclosed including categorizing a first and second data element in a database with a first attribute and second attribute, respectively, of a first dimension, a dimension being an aspect of a situation, problem, or thing. The first and second data elements are categorized with a first attribute and a second attribute of a second dimension, the second dimension being different from the first dimension. Analyzing the first and second attribute of the first dimension and the first and second attribute of the second dimension to determine a ratio of similarity and dissimilarity; calculating a composite score of the ratio of the first dimension and the ratio of the second dimension; and generating and storing a link between the first and second data element when the composite score is within numerical limits.

Abstract: Systems and methods for dynamic computer aided innovation via multidimensional complementary difference recommendation and exploration are disclosed including categorizing a first and second data element in a database with a first attribute and second attribute, respectively, of a first dimension, a dimension being an aspect of a situation, problem, or thing. The first and second data elements are categorized with a first attribute and a second attribute of a second dimension, the second dimension being different from the first dimension. Analyzing the first and second attribute of the first dimension and the first and second attribute of the second dimension to determine a ratio of similarity and dissimilarity; calculating a composite score of the ratio of the first dimension and the ratio of the second dimension; and generating and storing a link between the first and second data element when the composite score is within numerical limits.

Abstract: Systems and methods for dynamic computer aided innovation via multidimensional complementary difference recommendation and exploration are disclosed including categorizing a first and second data element in a database with a first attribute and second attribute, respectively, of a first dimension, a dimension being an aspect of a situation, problem, or thing. The first and second data elements are categorized with a first attribute and a second attribute of a second dimension, the second dimension being different from the first dimension. Analyzing the first and second attribute of the first dimension and the first and second attribute of the second dimension to determine a ratio of similarity and dissimilarity; calculating a composite score of the ratio of the first dimension and the ratio of the second dimension; and generating and storing a link between the first and second data element when the composite score is within numerical limits.

Abstract: Systems and methods for dynamic computer aided innovation via multidimensional complementary difference recommendation and exploration are disclosed including categorizing a first and second data element in a database with a first attribute and second attribute, respectively, of a first dimension, a dimension being an aspect of a situation, problem, or thing. The first and second data elements are categorized with a first attribute and a second attribute of a second dimension, the second dimension being different from the first dimension. Analyzing the first and second attribute of the first dimension and the first and second attribute of the second dimension to determine a ratio of similarity and dissimilarity; calculating a composite score of the ratio of the first dimension and the ratio of the second dimension; and generating and storing a link between the first and second data element when the composite score is within numerical limits.

Abstract: Apparatus, systems, and methods are provided to generate markings on the surface of a die or substrate. The markings represent information. The markings can be annealed onto the surface of the die or substrate using a laser. Another embodiment can use an out-of-focus laser beam to mark a solder resist material.

Abstract: Apparatus, systems, and methods are provided to generate markings on the surface of a die or substrate. The markings represent information. The markings can be annealed onto the surface of the die or substrate using a laser. Another embodiment can use an out-of-focus laser beam to mark a solder resist material.