Abstract: Embodiments provided herein relate to methods and compositions for next generation sequencing. Some embodiments include the preparation of a template library from a target nucleic acid using one-sided transposition, sequencing the template library, and capturing the contiguity information.

Abstract: The invention relates to a method for producing a plastic granulate (16), in which a process fluid (12) is contained in a process chamber (10) where an underwater granulation takes place and the process fluid in the process chamber has a temperature greater than 120° C. A process pressure of at least 2.0 bar is obtained in the process chamber, at which a granulation of the plastic strands (14) into plastic granulate occurs. From the process chamber, a mixture (18) of process fluid and plastic granulate is diverted into a first cooling zone (25) during cooling of the plastic granulate, while maintaining the process pressure. In a first separating device (22), the plastic granulate is separated from the process fluid under process pressure. In the process chamber, the process fluid has a temperature in the range from 120° C. to 160° C., and the process pressure obtained there is greater than the pressure of the vapour pressure curve of the process fluid.

Abstract: The invention relates to a method for producing a plastic granulate (16), in which a process fluid (12) is contained in a process chamber (10) where an underwater granulation takes place and the process fluid in the process chamber has a temperature greater than 120° C. A process pressure of at least 2.0 bar is obtained in the process chamber, at which a granulation of the plastic strands (14) into plastic granulate occurs. From the process chamber, a mixture (18) of process fluid and plastic granulate is diverted into a first cooling zone (25) during cooling of the plastic granulate, while maintaining the process pressure. In a first separating device (22), the plastic granulate is separated from the process fluid under process pressure. In the process chamber, the process fluid has a temperature in the range from 120° C. to 160° C., and the process pressure obtained there is greater than the pressure of the vapour pressure curve of the process fluid.

Abstract: The invention relates to a method and a device for producing and treating plastic pellets. According to said method, a melt of the plastic material is granulated to give pellets, the pellets are cooled in a cooling fluid, the pellets are separated from the cooling fluid and the pellets are crystallized. The device according to the invention is characterized by comprising a control unit which monitors the crystallization step and controls the method in such a manner that, in case of a disturbance of crystallization, the pellets are supplied to an intermediate storage alter separation of the pellets from the cooling fluid and, as soon as the disturbance is removed, the pellets temporarily stored in the intermediate storage are supplied to crystallization and are crystallized.

Abstract: A process for production of polyethylene terephthalate pellets includes the steps of: production of a melt of polyethylene terephthalate material; production of polyethylene terephthalate pellets from the melt by means of underwater pelletization into a process fluid; removal of the polyethylene terephthalate pellets from the site of underwater pelletization in the process fluid; crystallization of the polyethylene terephthalate pellets in the process fluid, wherein a surface of the polyethylene terephthalate pellets is crystallized and an interior of the polyethylene terephthalate pellets remains amorphous.

Abstract: The invention relates to a method and a device for producing and treating plastic pellets. According to said method, a melt of the plastic material is granulated to give pellets, the pellets are cooled in a cooling fluid, the pellets are separated from the cooling fluid and the pellets are crystallized. The device according to the invention is characterized by comprising a control unit which monitors the crystallization step and controls the method in such a manner that, in case of a disturbance of crystallization, the pellets are supplied to an intermediate storage alter separation of the pellets from the cooling fluid and, as soon as the disturbance is removed, the pellets temporarily stored in the intermediate storage are supplied to crystallization and are crystallized.

Abstract: The invention relates to a method and a device for producing and treating plastic pellets. According to said method, a melt of the plastic material is granulated to give pellets, the pellets are cooled in a cooling fluid, the pellets are separated from the cooling fluid and the pellets are crystallized. The device according to the invention is characterized by comprising a control unit which monitors the crystallization step and controls the method in such a manner that in case of a disturbance of crystallization the pellets are supplied to an intermediate storage after separation of the pellets from the cooling fluid and as soon as the disturbance is removed, the pellets temporarily stored in the intermediate storage are supplied to crystallization and are crystallized.

Abstract: A process for production of polyethylene terephthalate pellets includes the steps of: production of a melt of polyethylene terephthalate material; production of polyethylene terephthalate pellets from the melt by means of underwater pelletization into a process fluid; removal of the polyethylene terephthalate pellets from the site of underwater pelletization in the process fluid; crystallization of the polyethylene terephthalate pellets in the process fluid, wherein a surface of the polyethylene terephthalate pellets is crystallized and an interior of the polyethylene terephthalate pellets remains amorphous.

Abstract: The invention relates to a method for granulating and crystallizing thermoplastic polymers, the granules being contained in a liquid after granulation of a polymer melt and the granules being nucleated in the liquid once the pressure of the liquid is increased and the temperature of the liquid is increased, and the granules being crystallized in the liquid once the pressure of the liquid is again increased and the temperature of the liquid is again increased. According to the invention, the granules are removed from the liquid once the pressure and the temperature of the liquid containing the granules have been reduced.

Abstract: The invention relates to a method for granulating plastic having a high softening temperature, in particular of above 120° C., using a perforated plate for producing strands of molten plastic and a subsequent process chamber containing a process fluid and including a chopping device, from which a mixture of process fluid and granulate is then discharged into a cooling section for cooling said granulate. The process chamber is filled with a process fluid, in particular water, at a temperature of more than 120° C. and at a pressure of over 2 bar, and the mixture of process fluid and granulate, whose pressure will be maintained as it passes through the cooling section, will then be supplied to a separator in which the granulate, which has first been directed through a pressure lock for bringing it down to ambient pressure, will then be separated from the process fluid and discharged.

Abstract: A drop pelletizing device and method for producing pellets from a low-viscosity plastic melt are provided. The drop pelletizing device can include a die plate with holes, in which the plastic melt can be subjected to a harmonic pressure oscillation such that the plastic melt emerging from the holes forms individual pellet droplets, a pressure vessel, in which prevails an overpressure above the ambient pressure, a discharge device adapted to discharge the individual pellet droplets from the pressure vessel and to reduce the overpressure, a separator adapted to separate the individual pellet droplets from the coolant; and at least one circulating device adapted to agitate the coolant to separated and unclump the individual pellet droplets in the coolant and for producing turbulence within the coolant.

Abstract: The invention relates to an apparatus for continuous casting and granulating strands of a thermoplastic material which uses a nozzle head having a plurality of nozzle apertures of a maximum diameter of 4 mm each, and water-moistened guide means for cooling and guiding the plastic strands exiting the nozzle aperture via inlet rollers to the inlet of the cutting unit for chopping up the plastic strands into granules approx. 2-3 mm in length. The flow rate of the melt, with the strands being cooled down on their way from the nozzles via the guide means to the feed rollers of the cutting unit, of at least 100 nm/min in the central spatial region of the nozzle apertures will be increased to such an extent that the cutting unit will chop up the strands at a cutting rate of >2,000 cuts/s.

Abstract: A drop pelletizing device and method for producing pellets from a low-viscosity plastic melt are provided. The drop pelletizing device can include a die plate with holes, in which the plastic melt can be subjected to a harmonic pressure oscillation such that the plastic melt emerging from the holes forms individual pellet droplets, a pressure vessel, in which prevails an overpressure above the ambient pressure, a discharge device adapted to discharge the individual pellet droplets from the pressure vessel and to reduce the overpressure, a separator adapted to separate the individual pellet droplets from the coolant; and at least one circulating device adapted to agitate the coolant to separated and unclump the individual pellet droplets in the coolant and for producing turbulence within the coolant.

Abstract: The invention relates to a method for granulating and crystallizing thermoplastic polymers, the granules being contained in a liquid after granulation of a polymer melt and the granules being nucleated in the liquid once the pressure of the liquid is increased and the temperature of the liquid is increased, and the granules being crystallized in the liquid once the pressure of the liquid is again increased and the temperature of the liquid is again increased. According to the invention, the granules are removed from the liquid once the pressure and the temperature of the liquid containing the granules have been reduced.

Abstract: The invention relates to a method and a device for producing and treating plastic pellets. According to said method, a melt of the plastic material is granulated to give pellets, the pellets are cooled in a cooling fluid, the pellets are separated from the cooling fluid and the pellets are crystallized. The device according to the invention is characterized by comprising a control unit which monitors the crystallization step and controls the method in such a manner that in case of a disturbance of crystallization the pellets are supplied to an intermediate storage after separation of the pellets from the cooling fluid and as soon as the disturbance is removed, the pellets temporarily stored in the intermediate storage are supplied to crystallization and are crystallized.

Abstract: A method for manufacturing a crystallizable plastic material is disclosed. The process includes the step of melting an amorphous plastic material, pelletizing the material, crystallizing it and then post condensing the plastic material with the proviso that the plastic material is not subjected to heating after the melting step but prior to the crystallization step and then subjecting the plastic material to sieving after the crystallization step.