Abstract: The invention relates to a method and a computer program for segmentation of optical coherence tomography images of the retina comprising the steps of: a) Acquiring image data comprising a portion of the vitreous and a portion of the retina recorded with optical coherence tomography, wherein the portion of the retina comprises at least a portion of the optical nerve head, wherein the image data comprises pixels with associated pixel values; b) Providing a contour with a predefined initial shape and an initial position on the image data; c) Adjusting the shape and/or the position of the contour on the image data such that the adjusted contour separates the image data in a first region comprising the vitreous and a region comprising the retina, wherein the shape and position of the contour is adjusted with an optimization method, d) wherein the optimization method minimizes a contour-associated energy that depends on the contour shape, the contour position and the image data, wherein the contour-associated ener