Abstract: Provided are novel lipid nanoparticles for delivering nucleic acids such as mRNA. Also provided are methods of making and using lipid nanoparticles for delivering nucleic acids such as mRNA.

Abstract: The present disclosure relates to tagged nucleoside-5?-oligophosphates having a positively charged polymer tag structure and components thereof. Such nucleoside-5?-oligophosphates are useful, for example, in nanopore-based sequencing-by-synthesis applications. Also disclosed herein are nanopore constructs engineered to have additional negatively-charged moieties in the channel of the nanopore. Such nanopores can be useful, for example, for providing a repellant force against template and/or primer nucleic acids inserting into the pore during a nucleic sequence-by-synthesis process. The tagged nucleoside-5?-oligophosphates and nanopores disclosed herein can be used together to provide nanopore-based nucleic acid sequencing-by-synthesis systems and processes having reduced background tag levels and improved throughput.

Abstract: Provided are novel lipid nanoparticles for delivering nucleic acids such as mRNA. Also provided are methods of making and using lipid nanoparticles for delivering nucleic acids such as mRNA.

Abstract: A system and methods that enables and utilizes the conversion of an individual's ability to access multiple secure accounts to establish and validate the individual's digital primary identity. The control for the digital primary identity validation is user-centric so that the individual controls the amount of information supplied and therefore the level of assurance achievable. The level of assurance can be added to the digital primary identity. An identity card for digital primary identity validation can be used online or in-person. Validation and level of assurance is reviewed regularly or continuously. Levels of assurance can be increased by validating primary sources of identification.

Abstract: A system and methods that enables and utilizes the conversion of an individual's ability to access multiple secure accounts to establish and validate the individual's digital primary identity. The control for the digital primary identity validation is user-centric so that the individual controls the amount of information supplied and therefore the level of assurance achievable. The level of assurance can be added to the digital primary identity. An identity card for digital primary identity validation can be used online or in-person. Validation and level of assurance is reviewed regularly or continuously. Levels of assurance can be increased by validating primary sources of identification.

Abstract: An automated system utilizes a networked computer system such as the Internet for initiating and managing mergers and acquisitions of businesses between third parties. A host computer system connected to the network hosts a site operated on behalf of an investment banking firm. The site includes a secured login area, a seller registration area, a buyer registration area, a unique home page area for each registered seller and registered buyer, and a secure administration area. In each registration area a user enters profile information and is registered as a registered seller or registered buyer. The registered seller also provides detailed confidential information about a business to be offered for sale in addition to the seller profile. The unique home page area displays information and communications relating to opportunities matching the profile for that registered seller or buyer.