Abstract: A biosensor is produced by depositing thick film pastes by screen-printing on a substrate to produce thick-film conductivity electrodes, and immobilizing enzymes on the electrodes. In specific embodiments, the substrate is an unfired ceramic, a fired ceramic or a plastic, the paste is a noble metal paste, enzyme immobilization is with a bifunctional reagent, a plurality of different enzymes are immobilized on different electrodes, and/or the electrodes are printed as parallel lines or in the image of a comb or concentric circles. A multi-dimensional biosensor is prepared by forming thick film paste electrodes containing an immobilized enzyme on a plurality of separate substrates, disposing the separate substrates one on top of the other in a vertical stack, pressing the stacked substrates together and curing the paste.