Abstract: This disclosure provides systems, methods, and compositions for site specific genetic engineering comprising the use of CRISPR effectors and trans-splicing. The disclosure also relates to methods of using the systems and compositions for treating diseases as well as diagnostics.

Abstract: This disclosure provides systems, methods, and compositions for RNA-guided RNA-targeting CRISPR effectors for the treatment of diseases, and for use as diagnostics. In addition, nucleotide deaminase functionalized CRISPR systems for RNA editing RNA knockdown, viral resistance, splicing modulation, RNA tracking, translation modulation, and epi-transcriptomic modifications are disclosed.

Abstract: A magnetic resonance RF field is produced in a magnetic resonance imaging system that includes a main magnetic field apparatus and an object-bearing table movable relative to the main magnetic field apparatus and on which a local coil system is arranged. The local coil system includes a plurality of transmission elements. A current location of the object-bearing table relative to the main magnetic field apparatus is established. The transmission elements are automatically connected based on the current location of the object-bearing table.

Abstract: Methods are provided for measurement of RF excitation pulses by a magnetic resonance device. The methods include the following acts: (1) sending of an RF excitation pulse by a radio-frequency system of the magnetic resonance device, (2) triggering of a receive event for capturing the RF excitation pulse by the control device of the magnetic resonance device, and (3) capturing of the sent RF excitation pulse in the form of excitation data by the radio frequency system. The excitation data may be used for checking process execution sequences.

Abstract: A gradient pulse generator generates reference current signals for a plurality of gradient coils of a gradient coil system of a magnetic resonance system and supplies each of the reference current signals to a controller assigned to one of the gradient coils. Also supplied to the respective controller is an actual current signal that is characteristic of the current flowing in the respective gradient coil. Each of the controllers generates a control signal and accordingly drives a gradient power amplifier assigned to the respective gradient coil. The gradient power amplifiers apply a current to the gradient coils assigned to the gradient power amplifiers in accordance with the generated control signals. Each of the controllers is also supplied with the reference current signal or the actual current signal of at least one other gradient coil or the time derivative of the reference current signal or the actual current signal.

Abstract: A controller of a magnetic resonance system determines an activation signal vector based on a target field predetermined for the controller in conjunction with field characteristics of field coils known to the controller. The activation signal vector includes a respective activation signal for each field coil. The controller determines the activation signal vector such that within a predetermined examination volume of the magnetic resonance system, any deviation of an ideal field that would result if ideal coils were subjected to the activation signals of the activation signal vector from the target field is minimized. The controller determines a compensation signal vector based on the activation signal vector in conjunction with eddy current characteristics of the field coils known to the controller. The compensation signal vector is used to minimize a deviation of an actual field from the target field within the predetermined examination volume of the magnetic resonance system.

Abstract: The embodiments relate to methods for measurement of RF excitation pulses by a magnetic resonance device including the following acts: (1) sending of an RF excitation pulse by a radio-frequency system of the magnetic resonance device, (2) triggering of a receive event for capturing the RF excitation pulse by the control device of the magnetic resonance device, and (3) capturing of the sent RF excitation pulse in the form of excitation data by the radio frequency system. In certain embodiments, the excitation data is used for checking process execution sequences.

Abstract: A magnetic resonance apparatus including transmission antennas that may be actuated in parallel by a control device of the magnetic resonance apparatus may be operated in a group mode. In the group mode, the transmission antennas are grouped into groups of transmission antennas. The actuation signals of transmission antennas within the respective group are in a respectively predefined relationship relative to one another. A respective group actuation signal for each of the groups of transmission antennas is prescribed for the control device by an operator. The control device carries out checks as to whether a group exposure value established based on the group actuation signals lies below a maximum admissible group exposure limit. If this is the case, the control device establishes the actuation signals for the individual transmission antennas based on the group actuation signals. If this is not the case, the control device carries out another measure.

Abstract: A magnetic resonance RF field is produced in a magnetic resonance imaging system that includes a main magnetic field apparatus and an object-bearing table movable relative to the main magnetic field apparatus and on which a local coil system is arranged. The local coil system includes a plurality of transmission elements. A current location of the object-bearing table relative to the main magnetic field apparatus is established. The transmission elements are automatically connected based on the current location of the object-bearing table.

Abstract: A controller of a magnetic resonance system determines an activation signal vector based on a target field predetermined for the controller in conjunction with field characteristics of field coils known to the controller. The activation signal vector includes a respective activation signal for each field coil. The controller determines the activation signal vector such that within a predetermined examination volume of the magnetic resonance system, any deviation of an ideal field that would result if ideal coils were subjected to the activation signals of the activation signal vector from the target field is minimized. The controller determines a compensation signal vector based on the activation signal vector in conjunction with eddy current characteristics of the field coils known to the controller. The compensation signal vector is used to minimize a deviation of an actual field from the target field within the predetermined examination volume of the magnetic resonance system.

Abstract: A gradient pulse generator generates reference current signals for a plurality of gradient coils of a gradient coil system of a magnetic resonance system and supplies each of the reference current signals to a controller assigned to one of the gradient coils. Also supplied to the respective controller is an actual current signal that is characteristic of the current flowing in the respective gradient coil. Each of the controllers generates a control signal and accordingly drives a gradient power amplifier assigned to the respective gradient coil. The gradient power amplifiers apply a current to the gradient coils assigned to the gradient power amplifiers in accordance with the generated control signals. Each of the controllers is also supplied with the reference current signal or the actual current signal of at least one other gradient coil or the time derivative of the reference current signal or the actual current signal.

Abstract: In a method and apparatus to determine a predetermined signal amplitude of an examination subject in an MR measurement in which multiple RF pulses are radiated into the examination subject in a pulse sequence in a pulse series with a repetition time TR that is smaller than the T2 times of the examination subject, a target magnetization is established for a predetermined point in time after radiation of the respective RF pulse for essentially all RF pulses from the pulse series, a target flip angle and a target phase are determined for different regions of the examination subject for essentially all RF pulses depending on the respective target magnetization, a respective amplitude and phase response is determined for essentially all RF pulses to generate the respective target magnetization after radiation of the respective RF pulse, and the RF pulses with the respective determined amplitude and phase response are radiated into the examination subject.

Abstract: The invention relates to a sparger for introducing a gas or gas mixture into a liquid and to a method for sparging liquids. The sparger according to the invention comprises a cavity, a gas inlet for routing a gas into the cavity and two or more faces, which are or can be pressed onto one another in a positive fashion such that a gas pressed into the cavity through the gas inlet escapes through the gaps occurring between the pressed together faces.

Abstract: Devices, systems, methods, and other embodiments associated with magnetic resonance imaging (MRI) are described. In one embodiment, an apparatus includes an RF coil for use in multi-nuclear excitation in magnetic resonance imaging (MRI). The RF coil includes a set of two or more L-C coils. Members of the set of two or more L-C coils have individual resonance frequencies. An RF amplifier is placed near the RF coil. The RF amplifier is controllable to selectively produce the individual resonance frequency of a member of the set of two or more L-C coils based, at least in part, on a digital input provided to the RF amplifier.

Abstract: A magnetic resonance device has a patient bed able to be automatically moved by means of a drive apparatus into a patient chamber, as well as a magnet arrangement for creating a magnetic field in the interior of the patient chamber. The drive apparatus is operated so as to move the patient bed at a speed determined as a function of the distribution of the magnetic field in the direction of movement of the patient bed.

Abstract: The present embodiments relate to a system and a method for operating an imaging system, where a plurality of subvolumes of an examination volume of an examination object to be examined with the system is examined. The examination volume is assembled from the plurality of subvolumes, where to examine the subvolumes, at least one HF pulse is transmitted in each case. The at least one HF pulse is optimized for the subvolume that is to be examined therewith respect to specifications and basic conditions applicable for the subvolume.

Abstract: In a method for adjustment of a B1 field in a magnetic resonance apparatus, the position of the measurement subject relative to a coordinate system is determined from a plurality of measurement subjects with a morphological magnetic resonance measurement. Respective tissue types of the measurement subject are determined using three-dimensional measurement subject data and a segmentation of the measurement subject into regions is effected using the tissue types having known dielectric properties. The segmentation and the association for all positions of the measurement subject are recorded for all subjects in respective entries of a database. A radio-frequency simulation for transmission coils is implemented with the entries of the database wherein electrical field distributions and/or B1 field distributions related to spatial elements are determined and are entered as results into the database, that is then used further magnetic resonance examinations.

Abstract: In a method and apparatus to determine a predetermined signal amplitude of an examination subject in an MR measurement in which multiple RF pulses are radiated into the examination subject in a pulse sequence in a pulse series with a repetition time TR that is smaller than the T2 times of the examination subject, a target magnetization is established for a predetermined point in time after radiation of the respective RF pulse for essentially all RF pulses from the pulse series, a target flip angle and a target phase are determined for different regions of the examination subject for essentially all RF pulses depending on the respective target magnetization, a respective amplitude and phase response is determined for essentially all RF pulses to generate the respective target magnetization after radiation of the respective RF pulse, and the RF pulses with the respective determined amplitude and phase response are radiated into the examination subject.

Abstract: In a method for controlling a magnetic resonance system having a radio-frequency antenna structure and a number of individually controllable transmission channels, respective parallel radio-frequency signals are emitted via the transmission channels for generation of a desired radio-frequency field distribution in at least one specific volume region within an examination volume of the magnetic resonance system. A digital signal is generated for each of the transmission channels and is modulated on a carrier frequency. The radio-frequency signal so generated is transmitted via a radio-frequency signal path to the radio-frequency antenna structure and is amplified therein in a radio-frequency power amplifier.

Abstract: Described is a reactor for irradiating ultraviolet light into a fluid reaction medium (3). The reactor consists of at least one housing (15) which encloses a tubular cavity, with a radiation source (1) for generating ultraviolet light and an inner tube (2) which, together with the housing (15), forms an irradiation chamber (26) which, in particular, is of annular shape, the irradiation chamber (26) being connected at least with an inlet (13) and an outlet (14) for the reaction medium (3) and is perfused by reaction medium (3) in the longitudinal direction of the tube (2), the irradiation chamber (26) being equipped with means (6, 25) for generating an additional radial flow routing of the reaction medium (3).