Abstract: The present disclosure provides an expression vector and a method for generating the expression vector. The expression vector includes one or more inserted foreign DNA sequences, including human growth hormone (hGH) and a plurality of therapeutic genes, where the one or more inserted foreign DNA sequences are fused with intracellular or extracellular secretion sequences; a promoter, configured to drive expression of the one or more inserted foreign DNA sequences; an insulator, configured for stable and safety expression of the one or more inserted foreign DNA sequences; a marker gene, configured to encoding one or more corresponding proteins to indicate the expression of the one or more inserted foreign DNA sequences; and a regulator element, configured to control expression of the hGH and the plurality of therapeutic genes under a controllable condition.

Abstract: An expression vector is provided. The vector includes a promoter configured to drive the expression of the transgene in the cell. The vector also includes a tag sequence encoding a tag peptide directing the protein of the expressed transgene to a pre-determined location. The vector further includes a cleavage sequence encoding a peptide that is recognizable by a protease and a marker gene configured to encoding a protein to indicate the expression of the transgene.

Abstract: An expression vector is provided. The vector includes a promoter configured to drive the expression of the transgene in the cell. The vector also includes a tag sequence encoding a tag peptide directing the protein of the expressed transgene to a pre-determined location. The vector further includes a cleavage sequence encoding a peptide that is recognizable by a protease and a marker gene configured to encoding a protein to indicate the expression of the transgene.

Abstract: This invention is related to a process for filtering gases or liquids for removing liquid or solid impurities and is characterized in that one uses for filtering a fibre material which partly exhibits a layer structure, said fibres along the main part of the total fibre length exhibiting a direction which deviates less than 45.degree. from the direction of a main direction plane of said fibres, said filtering being performed so that the filtered medium is brought to flow away from the filter material through an outflow area in the filter material in which the flow direction is essentially parallel with the direction of the main direction plane of the fibres.