Patents by Inventor Fred P. Milanovich
Fred P. Milanovich has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20190255531Abstract: Method of performing a droplet-based assay. The method may include obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer, performing a reaction in the droplets while packed closely together in the monolayer; and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.Type: ApplicationFiled: May 6, 2019Publication date: August 22, 2019Applicant: Bio-Rad Laboratories, Inc.Inventors: Benjamin J. HINDSON, Kevin D. NESS, Billy W. COLSTON, JR., Fred P. MILANOVICH, Donald A. MASQUELIER
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Patent number: 10279350Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.Type: GrantFiled: November 30, 2016Date of Patent: May 7, 2019Assignee: Bio-Rad Laboratories, Inc.Inventors: Benjamin J. Hindson, Kevin D. Ness, Billy W. Colston, Jr., Fred P. Milanovich, Donald A. Masquelier
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Patent number: 9901927Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.Type: GrantFiled: November 30, 2016Date of Patent: February 27, 2018Assignee: Bio-Rad Laboratories, Inc.Inventors: Benjamin J. Hindson, Kevin D. Ness, Billy W. Colston, Jr., Fred P. Milanovich, Donald A. Masquelier
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Publication number: 20170144161Abstract: Methods of generating droplets. In an exemplary method, a device including a sample well, a carrier well, a droplet well, and a plurality of microfluidic channels is selected. The microfluidic channels include a first channel, a second channel, and a third channel. A discrete volume of sample-containing fluid is placed into the sample well, and a discrete volume of carrier fluid is placed into the carrier well. A pressure differential is created after placing the discrete volumes, to cause fluid flow. Sample-containing fluid flows from the sample well to a droplet-generation region of the device via the first channel. Carrier fluid flows from the carrier well to the droplet-generation region via the second channel. Sample-containing droplets and carrier fluid flow from the droplet-generation region to the droplet well via the third channel.Type: ApplicationFiled: November 30, 2016Publication date: May 25, 2017Inventors: Benjamin J. Hindson, Kevin D. Ness, Billy W. Colston, JR., Fred P. Milanovich, Donald A. Masquelier, Anthony J. Makarewicz, JR.
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Patent number: 9052255Abstract: An autonomous monitoring system for monitoring for bioagents. A collector gathers the air, water, soil, or substance being monitored. A sample preparation means for preparing a sample is operatively connected to the collector. A detector for detecting the bioagents in the sample is operatively connected to the sample preparation means. One embodiment of the present invention includes confirmation means for confirming the bioagents in the sample.Type: GrantFiled: May 24, 2010Date of Patent: June 9, 2015Assignee: Lawrence Livermore National Security, LLCInventors: Richard G. Langlois, Fred P. Milanovich, Billy W. Colston, Jr., Steve B. Brown, Don A. Masquelier, Raymond P. Mariella, Jr., Kodomudi Venkateswaran
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Patent number: 7993266Abstract: This invention provides an electronic proximity apparatus and a surveillance method using such an apparatus for alerting individuals that are exposed to a contagious disease. When a person becomes symptomatic and is diagnosed as positive for a given contagious agent, individuals that have recently maintained a threshold proximity with respect to an infected individual are notified and advised to seek immediate medial care. Treatment of individuals in the very early phases of infection (pre-symptomatic) significantly reduces contagiousness of the infected population first exposed to the contagious disease, thus preventing spread of the disease throughout the general population.Type: GrantFiled: February 26, 2003Date of Patent: August 9, 2011Assignee: Lawrence Livermore National Security, LLCInventors: Billy W. Colston, Jr., Fred P. Milanovich, Pedro Estacio, John Chang
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Publication number: 20110027781Abstract: An autonomous monitoring system for monitoring for bioagents. A collector gathers the air, water, soil, or substance being monitored. A sample preparation means for preparing a sample is operatively connected to the collector. A detector for detecting the bioagents in the sample is operatively connected to the sample preparation means. One embodiment of the present invention includes confirmation means for confirming the bioagents in the sample.Type: ApplicationFiled: May 24, 2010Publication date: February 3, 2011Inventors: Richard G. Langlois, Fred P. Milanovich, Billy W. Colston, JR., Steve B. Brown, Don A. Masquelier, Ray P. Mariella, Kodomudi Venkateswaran
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Patent number: 6905885Abstract: A portable pathogen detection system that accomplishes on-site multiplex detection of targets in biological samples. The system includes: microbead specific reagents, incubation/mixing chambers, a disposable microbead capture substrate, and an optical measurement and decoding arrangement. The basis of this system is a highly flexible Liquid Array that utilizes optically encoded microbeads as the templates for biological assays. Target biological samples are optically labeled and captured on the microbeads, which are in turn captured on an ordered array or disordered array disposable capture substrate and then optically read.Type: GrantFiled: June 12, 2001Date of Patent: June 14, 2005Assignee: The Regents of the University of CaliforniaInventors: Billy W. Colston, Matthew Everett, Fred P. Milanovich, Steve B. Brown, Kodumudi Vendateswaran, Jonathan N. Simon
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Patent number: 6699713Abstract: A portable polymerase chain reaction DNA amplification and detection system includes one or more chamber modules. Each module supports a duplex assay of a biological sample. Each module has two parallel interrogation ports with a linear optical system. The system is capable of being handheld.Type: GrantFiled: December 29, 2000Date of Patent: March 2, 2004Assignee: The Regents of the University of CaliforniaInventors: William J. Benett, James B. Richards, Paul L. Stratton, Dean R. Hadley, Fred P. Milanovich, Phil Belgrader, Peter L. Meyer
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Publication number: 20040038385Abstract: An autonomous monitoring system for monitoring for bioagents. A collector gathers the air, water, soil, or substance being monitored. A sample preparation means for preparing a sample is operatively connected to the collector. A detector for detecting the bioagents in the sample is operatively connected to the sample preparation means. One embodiment of the present invention includes confirmation means for confirming the bioagents in the sample.Type: ApplicationFiled: August 19, 2003Publication date: February 26, 2004Inventors: Richard G. Langlois, Fred P. Milanovich, Billy W. Colston, Steve B. Brown, Don A. Masquelier, Ray P. Mariella, Kodomudi Venkateswaran
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Publication number: 20030204130Abstract: This invention provides an electronic proximity apparatus and a surveillance method using such an apparatus for alerting individuals that are exposed to a contagious disease. When a person becomes symptomatic and is diagnosed as positive for a given contagious agent, individuals that have recently maintained a threshold proximity with respect to an infected individual are notified and advised to seek immediate medial care. Treatment of individuals in the very early phases of infection (pre-symptomatic) significantly reduces contagiousness of the infected population first exposed to the contagious disease, thus preventing spread of the disease throughout the general population.Type: ApplicationFiled: February 26, 2003Publication date: October 30, 2003Applicant: The Regents of the University of CaliforniaInventors: Billy W. Colston, Fred P. Milanovich, Pedro Estacio, John Chang
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Patent number: 6586233Abstract: A polymerase chain reaction system provides an upper temperature zone and a lower temperature zone in a fluid sample. Channels set up convection cells in the fluid sample and move the fluid sample repeatedly through the upper and lower temperature zone creating thermal cycling.Type: GrantFiled: March 9, 2001Date of Patent: July 1, 2003Assignee: The Regents of the University of CaliforniaInventors: William J. Benett, James B. Richards, Fred P. Milanovich
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Patent number: 6520034Abstract: A high air volume to low liquid volume aerosol collector. A high volume flow of aerosol particles is drawn into an annular, centripetal slot in a collector which directs the aerosol flow into a small volume of liquid pool contained is a lower center section of the collector. The annular jet of air impinges into the liquid, imbedding initially airborne particles in the liquid. The liquid in the pool continuously circulates in the lower section of the collector by moving to the center line, then upwardly, and through assistance by a rotating deflector plate passes back into the liquid at the outer area adjacent the impinging air jet which passes upwardly through the liquid pool and through a hollow center of the collector, and is discharged via a side outlet opening. Any liquid droplets escaping with the effluent air are captured by a rotating mist eliminator and moved back toward the liquid pool.Type: GrantFiled: April 3, 2002Date of Patent: February 18, 2003Assignee: The Regents of the University of CaliforniaInventors: Donald A. Masquelier, Fred P. Milanovich, Klaus Willeke
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Publication number: 20030027244Abstract: A portable pathogen detection system that accomplishes on-site multiplex detection of targets in biological samples. The system includes: microbead specific reagents, incubation/mixing chambers, a disposable microbead capture substrate, and an optical measurement and decoding arrangement. The basis of this system is a highly flexible Liquid Array that utilizes optically encoded microbeads as the templates for biological assays. Target biological samples are optically labeled and captured on the microbeads, which are in turn captured on an ordered array or disordered array disposable capture substrate and then optically read.Type: ApplicationFiled: September 27, 2002Publication date: February 6, 2003Applicant: The Regents of the University of CaliforniaInventors: Billy W. Colston, Matthew Everett, Fred P. Milanovich, Steve B. Brown, Kodumudi Venkateswaran, Jonathan N. Simon
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Publication number: 20030003441Abstract: A portable pathogen detection system that accomplishes on-site multiplex detection of targets in biological samples. The system includes: microbead specific reagents, incubation/mixing chambers, a disposable microbead capture substrate, and an optical measurement and decoding arrangement. The basis of this system is a highly flexible Liquid Array that utilizes optically encoded microbeads as the templates for biological assays. Target biological samples are optically labeled and captured on the microbeads, which are in turn captured on an ordered array or disordered array disposable capture substrate and then optically read.Type: ApplicationFiled: June 12, 2001Publication date: January 2, 2003Applicant: The Regents of the University of CaliforniaInventors: Billy W. Colston, Matthew Everett, Fred P. Milanovich, Steve B. Brown, Kodumudi Venkateswaran, Jonathan N. Simon
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Publication number: 20020191826Abstract: A portable polymerase chain reaction DNA amplification and detection system includes one or more chamber modules. Each module supports a duplex assay of a biological sample. Each module has two parallel interrogation ports with a linear optical system. The system is capable of being handheld.Type: ApplicationFiled: December 29, 2000Publication date: December 19, 2002Applicant: The Regents of the University of CaliforniaInventors: William J. Benett, James B. Richards, Paul L. Stratton, Dean R. Hadley, Fred P. Milanovich, Phil Belgrader, Peter L. Meyer
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Publication number: 20020127152Abstract: A polymerase chain reaction system provides an upper temperature zone and a lower temperature zone in a fluid sample. Channels set up convection cells in the fluid sample and move the fluid sample repeatedly through the upper and lower temperature zone creating thermal cycling.Type: ApplicationFiled: March 9, 2001Publication date: September 12, 2002Applicant: The Regents of the University of CaliforniaInventors: William J. Benett, James B. Richards, Fred P. Milanovich
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Patent number: 5466128Abstract: A miniature dual syringe-type pump assembly which has a high aspect ratio and which is remotely controlled, for use such as in a small diameter penetrometer cone or well packer used in water contamination applications. The pump assembly may be used to supply and remove a reagent to a water contamination sensor, for example, and includes a motor, gearhead and motor encoder assembly for turning a drive screw for an actuator which provides pushing on one syringe and pulling on the other syringe for injecting new reagent and withdrawing used reagent from an associated sensor.Type: GrantFiled: July 21, 1993Date of Patent: November 14, 1995Assignee: Regents of the University of CaliforniaInventors: Steve B. Brown, Fred P. Milanovich
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Patent number: 5122224Abstract: A crystal is grown in a constantly filtered solution which is flowed directly into the growing face of a crystal. In a continuous flow system, solution at its saturation temperature is removed from a crystal growth tank, heated above its saturation temperature, filtered, cooled back to its saturation temperature, and returned to the tank.Type: GrantFiled: October 30, 1989Date of Patent: June 16, 1992Assignee: The United States of America as represented by the United States Department of EnergyInventors: Kenneth E. Montgomery, Fred P. Milanovich
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Patent number: 4913519Abstract: An ice sensor for the remote rapid indication of ice formation or the presence of ice is a fiber optic "switch", activated by ice but not by water, and based on the difference in optical properties between water and ice. The approach is to construct a "fiber optic" which itself is the ice sensor. The fiber optic sensor (FOS) is designed so that no light is transmitted when water is present but as soon as ice begins to form, light is relayed. Thus ice switches on the light- In addition, limited quantitative information can be made available on the rate of ice formation. Alternatively the sensor can be formed of another type optical waveguide instead of an optical fiber. The ice sensor is formed by placing spaced stripes of a clad material on a fiber optic core, or other waveguide structure, where the clad has a refractive index close to ice and the core has an index greater than the clad but less than water.Type: GrantFiled: March 4, 1988Date of Patent: April 3, 1990Assignee: FiberChem Inc.Inventors: Stanley M. Klainer, Fred P. Milanovich