Patents by Inventor Fred Regnier

Fred Regnier has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220137063
    Abstract: A system and method is provided for validating the manufacturing process for the production of complex biological compositions, and particularly for providing process validation information for evaluation by a federal regulatory agency. The system and method continuously and chronologically assess the concentration of proteoforms within the biological composition as it is being produced in a fermentor. Samples from the fermentor are analyzed in a pre-selected array of analysis columns, with data generated by the columns being accumulated and evaluated, and particularly compared with data from previous stages in the production process. A continuous process validation system includes top-down and bottom-up analysis sectors, each including a plurality of different analysis columns that can be selected by the controller for a particular biological composition and a particular production process.
    Type: Application
    Filed: September 16, 2021
    Publication date: May 5, 2022
    Inventors: Fred Regnier, Jinhee Kim, Meena Narsimhan, Paul Dreier
  • Patent number: 10281474
    Abstract: The present invention relates to methods for continuous or near-continuous separation and purification of samples, particularly biological samples, to reduce the number or volume of non-targeted analytes in those samples to enable improved mass spectrometric analysis of analytes of interest, and apparatuses for conducting those methods, utilizing a transport agent with a core domain and a binding domain, the transport agent exceeding 200 kiloDaltons or 10 nm, and the binding domain targeted to the analytes of interest, in conjunction with size-exclusion based chromatography to separate the transport agent-analyte of interest complex from non-targeted analytes that are not bound, or are only non-specifically bound, to the transport agent.
    Type: Grant
    Filed: June 7, 2018
    Date of Patent: May 7, 2019
    Inventors: Fred Regnier, JinHee Kim
  • Publication number: 20180284129
    Abstract: The present invention relates to methods for continuous or near-continuous separation and purification of samples, particularly biological samples, to reduce the number or volume of non-targeted analytes in those samples to enable improved mass spectrometric analysis of analytes of interest, and apparatuses for conducting those methods, utilizing a transport agent with a core domain and a binding domain, the transport agent exceeding 200 kiloDaltons or 10 nm, and the binding domain targeted to the analytes of interest, in conjunction with size-exclusion based chromatography to separate the transport agent-analyte of interest complex from non-targeted analytes that are not bound, or are only non-specifically bound, to the transport agent.
    Type: Application
    Filed: June 7, 2018
    Publication date: October 4, 2018
    Inventors: Fred Regnier, JinHee Kim
  • Patent number: 10018635
    Abstract: The present invention relates to methods for continuous or near-continuous separation and purification of samples, particularly biological samples, to reduce the number or volume of non-targeted analytes in those samples to enable improved mass spectrometric analysis of analytes of interest, and apparatuses for conducting those methods, utilizing a transport agent with a core domain and a binding domain, the transport agent exceeding 200 kiloDaltons or 10 nm, and the binding domain targeted to the analytes of interest, in conjunction with size-exclusion based chromatography to separate the transport agent-analyte of interest complex from non-targeted analytes that are not bound, or are only non-specifically bound, to the transport agent.
    Type: Grant
    Filed: July 30, 2015
    Date of Patent: July 10, 2018
    Inventors: Fred Regnier, JinHee Kim
  • Publication number: 20160089669
    Abstract: The invention relates to a device for the rapid simultaneous collection, and, optionally, treatment or standardization, of multiple aliquots of a liquid biological sample such as blood from a single source drop or sample portion, the device comprising device comprising a cover, a spreading layer comprising a macroporous membrane adjacent to the cover, where the spreading layer comprises a transport portion and at least one accumulation portion in fluid connection with the transport portion, and at least one collection portion in fluid connection with each accumulation portion.
    Type: Application
    Filed: September 28, 2015
    Publication date: March 31, 2016
    Inventors: Fred Regnier, Jinhee Kim, Jiri Adamec, Timothy E. Woenker, Richard P. Zoltek
  • Publication number: 20160033526
    Abstract: The present invention relates to methods for continuous or near-continuous separation and purification of samples, particularly biological samples, to reduce the number or volume of non-targeted analytes in those samples to enable improved mass spectrometric analysis of analytes of interest, and apparatuses for conducting those methods, utilizing a transport agent with a core domain and a binding domain, the transport agent exceeding 200 kiloDaltons or 10 nm, and the binding domain targeted to the analytes of interest, in conjunction with size-exclusion based chromatography to separate the transport agent-analyte of interest complex from non-targeted analytes that are not bound, or are only non-specifically bound, to the transport agent.
    Type: Application
    Filed: July 30, 2015
    Publication date: February 4, 2016
    Inventors: Fred Regnier, JinHee Kim
  • Patent number: 9068995
    Abstract: The present invention comprises methods for determining the presence, amount, or concentration of analytes of interest including Vitamin D and other secosteroids from biological samples, through derivatization, with improved speed and ease of analysis and improved sensitivity to mass spectrometry. In a preferred embodiment, the present invention comprises methods for determining the presence, amount, or concentration of analytes of interest, including Vitamin D and other secosteroids from whole human blood, through derivatization, and using a plasma collection device to facilitate collection, separation, and preparation of the sample for derivatization and analysis.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: June 30, 2015
    Inventors: Fred Regnier, Jinhee Kim, Jiri Adamec, Timothy E. Woenker, Richard P. Zoltek, Wenchu Yang
  • Publication number: 20140273260
    Abstract: The present invention comprises methods for determining the presence, amount, or concentration of analytes of interest including Vitamin D and other secosteroids from biological samples, through derivatization, with improved speed and ease of analysis and improved sensitivity to mass spectrometry. In a preferred embodiment, the present invention comprises methods for determining the presence, amount, or concentration of analytes of interest, including Vitamin D and other secosteroids from whole human blood, through derivatization, and using a plasma collection device to facilitate collection, separation, and preparation of the sample for derivatization and analysis.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Applicant: Novilytic, LLC
    Inventors: Fred Regnier, Jinhee Kim, Jiri Adamec, Timothy E. woenker, Richard P. Zoltek, Wenchu Yang
  • Publication number: 20080050827
    Abstract: The present invention provides compositions and methods for enhanced detection and quantification of amino acids by derivatization. Also provided are compositions and methods for enhanced detection and quantification of peptides by derivatization.
    Type: Application
    Filed: July 2, 2007
    Publication date: February 28, 2008
    Inventors: Hamid Mirzaei, Fred Regnier, Wen-Chu Yang
  • Publication number: 20070259366
    Abstract: The present invention comprises a diagnostic platform. The platform has a substrate having plurality of hydrophobic wells formed thereon. The hydrophobic wells are directly printed onto the substrate in a test pattern and are adapted to contain a biological sample for conducting a diagnostic assay to identify analytes within the biological sample. The test pattern is divided into equally sized quadrants, each containing an equal number of hydrophobic wells. The hydrophobic wells may be in a single or double well arrangement, as well as may be connected by at least one microfluidic channel.
    Type: Application
    Filed: May 3, 2007
    Publication date: November 8, 2007
    Inventors: Greg Lawrence, Ignacio Sanchez, Fred Regnier, Ray DeGrella
  • Publication number: 20070054416
    Abstract: A sample holder comprises a substrate microfabricated to define a multiplicity of microscopic islands defining sample support surfaces. At least one sump separates adjacent island surfaces and inhibits transport of samples between adjacent island surfaces.
    Type: Application
    Filed: November 6, 2006
    Publication date: March 8, 2007
    Inventors: Fred Regnier, Noubar Afeyan, Marvin Vestal
  • Publication number: 20070023643
    Abstract: A method of probing a plurality of analyzer molecules distributed about a detection platform is disclosed. The method includes contacting a test sample to the plurality of analyzer molecules, scanning the plurality of analyzer molecules at a rate relating to a carrier frequency signal, and detecting the presence or absence of a biological molecule based at least in part upon the presence or absence of a signal substantially at a sideband of the carrier frequency signal. A molecule detection platform including a substrate and a plurality of targets positioned about the substrate is also disclosed. Specific analyzer molecules adapted to bind a specific analyte are immobilized about a first set of the targets. Nonspecific analyzer molecules are immobilized about a second set of the targets. The targets positioned about the substrate along at least a segment of a scanning pathway alternate between at least one of the first set and at least one of the second set.
    Type: Application
    Filed: February 1, 2006
    Publication date: February 1, 2007
    Inventors: David Nolte, Manoj Varma, Fred Regnier, Leilei Peng, Ming Zhao
  • Publication number: 20070003925
    Abstract: A planar array having plurality of biological recognition molecules including at least two types of biological recognition molecules distributed about a substrate is disclosed. A first type of biological recognition molecules is distributed according to a first frequency and a second type of biological recognition molecules is distributed according to a second frequency. Another planar array having a plurality of biological recognition molecules including at least two kinds of biological recognition molecules is disclosed. The recognition molecules are distributed about a substrate with first kind of biological recognition molecules distributed at a first height or depth relative to a surface of the substrate and a second kind of biological recognition molecules distributed at a second height or depth relative to the surface.
    Type: Application
    Filed: February 1, 2006
    Publication date: January 4, 2007
    Inventors: David Nolte, Manoj Varma, Fred Regnier, Leilei Peng
  • Publication number: 20070003436
    Abstract: A phase contrast quadrature interferometric method for determining the presence or absence of a target analyte in a sample. The method comprises using a laser beam having a wavelength ? and a waist wo to probe at least a portion of a substrate having a reflecting surface that has been exposed to the sample. The reflecting surface includes at least a first region having a layer of recognition molecules specific to the target analyte and a second region that does not include a layer of recognition molecules specific to the target analyte. The method further comprises measuring a time dependent intensity on a photodetector of a substantially only first quadrature at one of a pair of quadrature angles ?q of a reflected diffraction signal of the probe beam while probing the first region and the second region. An apparatus for phase-contrast quadrature interferometric detection of the presence or absence of a target molecule on a planar array, comprises a laser source for generating a probe beam.
    Type: Application
    Filed: February 1, 2006
    Publication date: January 4, 2007
    Inventors: David Nolte, Leilei Peng, Fred Regnier, Ming Zhao
  • Publication number: 20060256350
    Abstract: An apparatus for assessing topology of a surface of a target. The apparatus includes an optical source for generating a probe laser beam. The apparatus also includes means for scanning the probe laser beam across at least a portion of the surface of the target. The apparatus further includes a beamsplitter for redirecting a return signal toward means for detecting the return signal in a substantially quadrature condition, the return signal resulting from reflection of the probe laser beam off the surface of the target. A quadrature interferometric method for determining the presence or absence of a target analyte in a sample. The method comprises generating a laser probe beam having a wavelength ? and a waist wo to probe at least a portion of a substrate having a reflecting surface that has been exposed to the sample.
    Type: Application
    Filed: February 1, 2006
    Publication date: November 16, 2006
    Inventors: David Nolte, Manoj Varma, Fred Regnier
  • Publication number: 20060256676
    Abstract: A device for identifying analytes in a biological sample, including a substrate configured to bind the analyte and a detection system to determine the presence or absence of the analyte in the biological sample.
    Type: Application
    Filed: January 13, 2006
    Publication date: November 16, 2006
    Inventors: David Nolte, Fred Regnier, Leilei Peng
  • Patent number: 6794148
    Abstract: The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second “dimension”. The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
    Type: Grant
    Filed: December 5, 2001
    Date of Patent: September 21, 2004
    Assignee: PerSeptive Biosystems, Inc.
    Inventors: Satish Jindal, Fred Regnier, Kevin Williams, Noubar Afeyan, Sandeep Paliwal, David Evans, Aruna Pingali
  • Publication number: 20020150926
    Abstract: The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second “dimension”. The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
    Type: Application
    Filed: December 5, 2001
    Publication date: October 17, 2002
    Inventors: Satish Jindal, Fred Regnier, Kevin Williams, Noubar Afeyan, Sandeep Paliwal, David Evans, Aruna Pingali
  • Patent number: 6358692
    Abstract: The invention provides novel methods for screening a sample to select a ligand to a target of interest and for obtaining information about the ligand and its binding characteristics. Specifically, the claimed multi-dimensional methods involve combining a solution of heterogeneous ligands with the target of interest to screen the ligands on the basis of one or more binding characteristics. Ligands having the first binding characteristic bind to the target of interest thereby to form a target/ligand complex. The complex then optionally is separated from the unbound components using any of a variety of separation techniques, e.g., size exclusion. At least one of the complex or unbound components then is introduced to a second “dimension”. The second dimension is capable of separating components based upon a second binding characteristic. One then elutes the ligand having the desired binding characteristics.
    Type: Grant
    Filed: March 12, 1999
    Date of Patent: March 19, 2002
    Assignee: PerSeptive Biosystems, Inc.
    Inventors: Satish Jindal, Fred Regnier, Kevin Williams, Noubar Afeyan, Sandeep Paliwal, David Evans, Aruna Pingali
  • Patent number: 6170981
    Abstract: The present invention relates to an in situ micromachined mixer for microfluidic analytical systems. In a preferred embodiment, a 100 pL mixer for liquids transported by electroosmotic flow (EOF) is described. Mixing was achieved in multiple intersecting channels with a bimodal width distribution and varying lengths. Five &mgr;m width channels ran parallel to the direction of flow whereas larger 27 &mgr;m width channels ran back and forth through the network at a 45° angle. All channels were approximately 10 &mgr;m deep. It was observed that little mixing of confluent streams occurred in the 100 &mgr;m wide mixer inlet channel where mixing would be achieved almost exclusively by diffusion. In contrast, mixing was complete after passage through the channel network in the ≈200 &mgr;m length mixer. Solvent composition was altered by varying the voltage on solvent reservoirs. The high efficiency attained in this mixer was attributed to the presence of a 2 pL vortex in the center of the mixer.
    Type: Grant
    Filed: May 6, 1999
    Date of Patent: January 9, 2001
    Assignee: Purdue Research Foundation
    Inventors: Fred Regnier, Bing He