Patents by Inventor Fred Russell Kramer

Fred Russell Kramer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190225999
    Abstract: This invention discloses multi-part primers for primer-dependent nucleic acid amplification methods. Also disclosed are multiplex assay methods, related reagent kits, and oligonucleotides for such methods.
    Type: Application
    Filed: April 5, 2017
    Publication date: July 25, 2019
    Applicant: Rutgers, The State University of New Jersey
    Inventors: Salvatore A. E. MARRAS, Diana VARGAS-GOLD, Sanjay TYAGI, Fred Russell KRAMER
  • Publication number: 20110021361
    Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
    Type: Application
    Filed: April 6, 2010
    Publication date: January 27, 2011
    Applicant: University of Medicine and Dentistry of New Jersey
    Inventors: Alexander B. Chetverin, Fred Russell Kramer
  • Publication number: 20100197509
    Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
    Type: Application
    Filed: July 6, 2009
    Publication date: August 5, 2010
    Applicant: University of Medicine and Dentistry of New Jersey
    Inventors: Alexander B. Chetverin, Fred Russell Kramer
  • Publication number: 20030162210
    Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
    Type: Application
    Filed: December 31, 2002
    Publication date: August 28, 2003
    Applicant: PUBLIC HEALTH RESEARCH INSTITUTE OF THE CITY OF NEW YORK, INC., a New York Corporation
    Inventors: Alexander B. Chetverin, Fred Russell Kramer
  • Patent number: 6420539
    Abstract: The present invention provides a replicatable and hybridizable recombinant single-stranded RNA probe molecule comprising: a recognition sequence for the binding of an RNA-directed RNA polymerase; a sequence required for the initiation of product strand synthesis by the polymerase; and a heteroloqus RNA sequence inserted at a specific site in the internal region of the recombinant molecule and complementary to an oligo or polynucleotide of interest. This invention also provides methods for determining the presence of concentration of an oligo- or polynucleotide of interest in a sample and for simultaneously determining the presence or concentration of several different oligo- or polynucleotides of interest in a sample.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: July 16, 2002
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Fred Russell Kramer, Paul M. Lizardi, Eleanor Ann Miele, Donald R. Mills
  • Patent number: 6322971
    Abstract: Ligation methods for manipulating nucleic acid stands and oligonucleotides utilizing hybridization arrays of immobilized oligonucleotides. The oligonucleotide arrays may be plain or sectioned, comprehensive or non-comprehensive. The immobilized oligonucleotides may in some cases be binary oligonucleotides having constant as well as variable segments. Some embodiments include amplification of ligated products.
    Type: Grant
    Filed: September 30, 1998
    Date of Patent: November 27, 2001
    Assignee: The Public Health Research Institute of the city of New York, Inc.
    Inventors: Alexander B. C{acute over (h)}etverin, Fred Russell Kramer
  • Patent number: 6150097
    Abstract: Nucleic acid hybridization probes having a first conformation when not interacting with a target and a second conformation when interacting with a target, and having the ability to bring a label pair into touching contact in one conformation but not the other, are labeled with a non-FRET pair of chromophores and generate a fluorescent or absorbance signal. Kits may include such probes, and assays, including multiplex assays, may utilize such probes.
    Type: Grant
    Filed: December 12, 1997
    Date of Patent: November 21, 2000
    Assignee: The Public Health Research Institute of the City of New York, Inc.
    Inventors: Sanjay Tyagi, Fred Russell Kramer
  • Patent number: 6103463
    Abstract: A method of sorting mixtures of nucleic acid strands comprising hybridizing the strands to an array of immobilized oligonucleotides, each of which includes a constant segment adjacent to a variable segment. The constant segment of the immobilized oligonucleotides can be made complementary to the ends of strands obtained by digesting a double-stranded nucleic acid with a restriction enzyme and restoring the restriction sites, thereby permitting the sorting of strands according to their variable sequences adjacent to their constant terminal restored restriction sites.
    Type: Grant
    Filed: May 23, 1994
    Date of Patent: August 15, 2000
    Assignee: The Public Health Research Institute of the City of New York, Inc.
    Inventors: Alexander B. Chetverin, Fred Russell Kramer
  • Patent number: 5871976
    Abstract: This invention provides a method for producing a mutant virus or viroid, or a mutant recombinant RNA molecule comprising a single stranded virus genome or a single stranded viroid genome, a circular virus genome or a circular viroid genome, or a segment of a virus genome or a segment of a viroid genome which comprises incubating a recombinant single-stranded RNA molecule comprising a recognition sequence for the binding of an RNA directed RNA polymerase, a sequence for the initiation of product strand synthesis by the polymerase and a heterologous sequence of interest derived from a different RNA molecule inserted at a specific site in the internal region of the recombinant molecule under appropriate selective conditions and for a sufficient period of time permitting the selection of a mutant population, the heterologous sequence of interest comprising the RNA of the single stranded virus genome or the single stranded viroid genome, the circular virus genome or the circular viroid genome or a segment of the v
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: February 16, 1999
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Fred Russell Kramer, Eleanor Anne Miele, Donald Robert Mills