Patents by Inventor Fred W. Wagner
Fred W. Wagner has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20120156719Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: ApplicationFiled: January 15, 2009Publication date: June 21, 2012Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
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Patent number: 8148508Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: GrantFiled: August 23, 2010Date of Patent: April 3, 2012Assignee: NPS Pharmaceuticals, Inc.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Jin Seog Seo
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Patent number: 8003362Abstract: The invention provides methods and nucleic acid constructs to express clostripain. The source of the coding region for recombinantly expressed clostripain is Clostridium histolyticum.Type: GrantFiled: November 24, 2004Date of Patent: August 23, 2011Assignee: Medtronic Inc.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Barton Holmquist
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Publication number: 20110130556Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: ApplicationFiled: August 23, 2010Publication date: June 2, 2011Applicant: NPS PHARMACEUTICALS, INC.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Jin Seog Seo
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Patent number: 7781567Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: GrantFiled: November 24, 2004Date of Patent: August 24, 2010Assignee: NPS Pharmaceuticals, Inc.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Jin Seog Seo
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Patent number: 7498148Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: GrantFiled: November 21, 2007Date of Patent: March 3, 2009Assignee: Restoragen, Inc.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
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Publication number: 20080153129Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: ApplicationFiled: November 21, 2007Publication date: June 26, 2008Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
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Patent number: 7335486Abstract: The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.Type: GrantFiled: November 24, 2004Date of Patent: February 26, 2008Assignee: Restoragen, Inc.Inventors: Fred W. Wagner, Peng Luan, Yuannan Xia, Daniel Strydom, Edwin H. Merrifield, Mary J. Bossard, Barton Holmquist, Jin Seog Seo
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Patent number: 6461834Abstract: The invention provides a method of producing a polypeptide having a C-terminal &agr;-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an &agr;-carboxamide group (C-terminal “Arg-NH2”). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.Type: GrantFiled: December 16, 1998Date of Patent: October 8, 2002Assignee: Bionebraska, Inc.Inventors: Dan Dormady, Jay S. Stout, Daniel J. Strydom, Barton Holmquist, Fred W. Wagner
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Patent number: 6410707Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.Type: GrantFiled: January 2, 2001Date of Patent: June 25, 2002Assignee: BioNebraska, Inc.Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
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Patent number: 6403361Abstract: The method of the invention provides for the formation of a recombinant polypeptide which has been modified at the C-terminal end through the use of a transpeptidation process. The method is suitable for modifying recombinant polypeptides of any source including those which may be commercially available, those derived from recombinant single copy or multicopy polypeptide constructs, or those derived from single or multicopy recombinant fusion protein constructs. The transpeptidation reaction involves contacting an endopeptidase enzyme with a recombinant polypeptide to substitute an addition unit, of one or more amino acids, for a leaving unit, linked to a core polypeptide through a cleavage site recognized by the endopeptidase enzyme. Recombinant polypeptides derived from multicopy polypeptide constructs may be cleaved from the multicopy polypeptide at the N-terminal and C-terminal ends and simultaneously under go substitution of the leaving unit by the desired addition unit.Type: GrantFiled: February 17, 2000Date of Patent: June 11, 2002Assignee: Restoragen, Inc.Inventors: Fred W. Wagner, Jay Stout, Dennis Henriksen, Bruce Partridge, Shane Manning
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Publication number: 20010031856Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.Type: ApplicationFiled: January 2, 2001Publication date: October 18, 2001Applicant: Bio Nebraska, Inc.Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
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Patent number: 6251635Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.Type: GrantFiled: August 25, 1998Date of Patent: June 26, 2001Assignee: Bionebraska, Inc.Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
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Patent number: 6221617Abstract: A method and recombinant assay cell for detecting growth hormone releasing hormone (GHRH) in a sample, the method includes exposing a recombinant cell to the sample and measuring transcription of a reporter gene. A suitable recombinant cell includes a reporter gene operatively connected to a cAMP-responsive promoter and a GHRH-responsive protein whose binding to GHRH induces the production of cAMP. GHRH present in an assay sample results in the GHRH-responsive protein activating the production of cAMP, which then activates the c-AMP-responsive promoter to express the reporter protein. The amount of reporter protein produced is quantitatively correlated to the amount of GHRH in the sample. In one embodiment, the protein is a cell surface receptor for GHRH.Type: GrantFiled: December 5, 1997Date of Patent: April 24, 2001Inventors: Julie Heinrich, H. Edward Grotjan, Fred W. Wagner, Yuannan Xia
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Patent number: 6187579Abstract: The invention provides customized proteases (i.e., mutant enzymes), methods of making customized proteases, as well as methods of using customized proteases. The customized proteases of the invention are derived from the known proteases. Altered transacylation reactions include the capability to perform transacylation reactions not substantially catalyzed by the known protease or the capability to perform transacylation reactions with improved yields, or both. The methods of the invention provide for customized proteases through site specific or random mutagenesis of the active site amino acids of the known proteases. The invention also provides for methods of using the customized proteases to prepare a preselected transacylation products. The preselected transacylation products produced can be modified by substitution at the N-or C-terminal with nucleophiles such as L-amino acids, D-amino acids, amino acid amides, and radioactive amino acids.Type: GrantFiled: October 27, 1994Date of Patent: February 13, 2001Assignee: Carlsberg A/SInventors: Klaus Breddam, Morten C. Kielland-Brandt, Uffe Hasbo Mortensen, Kjeld Ove Olesen, Henning Ralf Stennicke, Fred W. Wagner
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Patent number: 6051399Abstract: A method for the production of C-terminal amidated recombinant peptides is provided. The method employs a recombinant protein construct having multiple copies of a target peptide linked by intraconnecting peptides. The intraconnecting peptides permit the multicopy construct to be selectively reacted to produce product peptides having a C-terminal .alpha.-carboxamide. A recombinant gene containing a DNA sequence coding for the recombinant protein construct and an expression cassette, an expression vector and a transformed cell including the recombinant gene are also provided.Type: GrantFiled: September 19, 1997Date of Patent: April 18, 2000Assignee: BioNebraska, Inc.Inventors: Jay S. Stout, Bruce E. Partridge, Dennis B. Henriksen, Barton Holmquist, Fred W. Wagner
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Patent number: 6037143Abstract: The method of the invention provides for the formation of a recombinant polypeptide which has been modified at the C-terminal end through the use of a transpeptidation process. The method is suitable for modifying recombinant polypeptides of any source including those which may be commercially available, those derived from recombinant single copy or multicopy polypeptide constructs, or those derived from single or multicopy recombinant fusion protein constructs. The transpeptidation reaction involves contacting an endopeptidase enzyme with a recombinant polypeptide to substitute an addition unit, of one or more amino acids, for a leaving unit, linked to a core polypeptide through a cleavage site recognized by the endopeptidase enzyme. Recombinant polypeptides derived from multicopy polypeptide constructs may be cleaved from the multicopy polypeptide at the N-terminal and C-terminal ends and simultaneously under go substitution of the leaving unit by the desired addition unit.Type: GrantFiled: November 7, 1997Date of Patent: March 14, 2000Assignee: BioNebraska, Inc.Inventors: Fred W. Wagner, Jay Stout, Dennis Henriksen, Bruce Partridge, Shane Manning
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Patent number: 5972656Abstract: Metal binding polypeptides which include an amino acid sequence coding for a variable region of a monoclonal antibody which immunoreacts with a mercury cation and nucleotides which include a nucleic acid sequence coding for the variable region are provided. The invention is also directed to fusion proteins which include a phage coat protein or portion thereof and the monoclonal antibody heavy chain variable region. The invention also provides bacteriophages which include the fusion protein in their coat. In addition, methods for detecting, removing, adding, or neutralizing mercuric cations in biological or inanimate systems through the use of the mercury binding polypeptides are provided.Type: GrantFiled: July 3, 1997Date of Patent: October 26, 1999Assignee: BioNebraska, Inc.Inventors: Osvaldo Lopez, Dwane E. Wylie, Fred W. Wagner
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Patent number: 5962270Abstract: A process for the recombinant preparation of a calcitonin fragment and the use of the fragment in the preparation of calcitonin and related analogs is provided. The process includes recombinantly forming a fusion protein which includes the calcitonin fragment linked to a carbonic anhydrase. The recombinantly formed fusion protein is subsequently cleaved to produce a polypeptide which includes the calcitonin fragment. A method for producing a calcitonin carba analog which includes condensing a desaminononapeptide with the recombinantly formed calcitonin fragment is also provided.Type: GrantFiled: February 6, 1996Date of Patent: October 5, 1999Assignee: Bionebraska, Inc.Inventors: Fred W. Wagner, Jay S. Stout, Dennis B. Henriksen, Bruce E. Partridge, Bart Holmquist, Julie A. Frank
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Patent number: 5945329Abstract: The invention provides customized proteases (i.e., mutant enzymes), methods of making customized proteases, as well as methods of using customized proteases. The customized proteases of the invention are derived from the known proteases. Altered transacylation reactions include the capability to perform transacylation reactions not substantially catalyzed by the known protease or the capability to perform transacylation reactions with improved yields, or both. The methods of the invention provide for customized proteases through site specific or random mutagenesis of the active site amino acids of the known proteases. The invention also provides for methods of using the customized proteases to prepare a preselected transacylation products. The preselected transacylation products produced can be modified by substitution at the N- or C-terminal with nucleophiles such as L-amino acids, D-amino acids, amino acid amides, and radioactive amino acids.Type: GrantFiled: July 23, 1997Date of Patent: August 31, 1999Assignee: Carlsberg A/SInventors: Klaus Breddam, Morten C. Kielland-Brandt, Uffe Hasbo Mortensen, Kjeld Ove Olesen, Henning Ralf Stennicke, Fred W. Wagner