Abstract: The present invention relates miRNA biomarkers from milk. e.g. exosome-derived microRNA from milk, as indicators of welfare in bovines. In particular, the present invention relates to the identification of heat stress in bovines allowing to propose management actions to improve productive animal welfare at the individual or the herd levels.

Abstract: A nutritional composition comprising miR-3141. miR-3141 or said nutritional composition for use as a medicament. Use of miR-3141 to modulate gene expression of one or more geneselected from Claudin 2 and SLC2A1. A method of producing said nutritional composition.

Abstract: A nutritional composition comprising miR-3126. miR-3126 or said nutritional composition for use as a medicament. Use of miR-3126 to modulate gene expression of one or more gene selected from: Ninein, Unc-13 homolog D, Sulfatase 2, IL-32, OXER1, TMEM127, Butyrophilin, Integrin subunit alpha 2, Integrin beta 1, tubulin alpha 4A, tubulin beta 6, tubulin beta 2A and tubulin beta 2B. A method of producing said nutritional composition.

Abstract: A nutritional composition comprising miR-3184. miR-3184 or said nutritional composition for use as a medicament. Use of miR-3184 to modulate gene expression of one or more gene selected from Lipin 2 or Insulin Like Growth Factor 2. A method of producing said nutritional composition.

Abstract: Methods for selecting and designing optimal nucleic acid-based probe for improving the sensitivity of detection of a nucleic acid-based target are disclosed herein. The capture probes generated from these methods show a significant improvement in the sensitivity of detection. Improved probes as well as microarrays and kits comprising these probes are disclosed herewith.

Abstract: This present invention relates methods for detecting the presence of nucleic acids in a sample. In these methods, neutral capture probes are exposed to a sample possibly containing complementary nucleic acid targets. The foregoing mixture is submitted to conditions that provide for the nucleic acid targets to bind with the neutral probes thereby generating hybrids. These hybrids are submitted to positively charged reporters such as atoms, molecules or macromolecules, which electrostatically bind to the hybrids. The complexes formed between reporters and hybrids are detected by a variety of detection methods. Kits for detecting the presence of nucleic acids in a sample are also disclosed herein.