Patents by Inventor Frederic Reymond

Frederic Reymond has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7615354
    Abstract: A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.
    Type: Grant
    Filed: May 4, 2001
    Date of Patent: November 10, 2009
    Assignee: Ecole Polytechnique Federale de Lausanne
    Inventors: Michel D. Faupel, Hubert H. Girault, Frederic Reymond, Alexandra Ros, Joel Stephane Rossier
  • Publication number: 20090178935
    Abstract: A method to optimize the amperometric detection in a microsystem consists in limiting the detection to times when the diffusion layer (18-20) of the analyte to detect remains smaller than the microchannel (7) height. The charge detected during the second part of the amperometric measurement (which corresponds to the integral of the measured current over the corresponding time period) can also be considered so as to remove the contribution of the capacitive current and, when applicable, of the current resulting from the reduction or oxidation of the analyte molecules present in a recess above the electrode at the beginning of the detection. A microfluidic amperometric sensor for performing the method comprises at least one microchannel (7) having at least one electrode (15-17), integrated in one wall of the microchannel, and having a characteristic length or radius which is smaller than half the microchannel height.
    Type: Application
    Filed: March 29, 2007
    Publication date: July 16, 2009
    Applicants: DIAGNOSWISS S.A., BIOMERIEUX S.A.
    Inventors: Frederic Reymond, Joel Stephane Rossier, Patrick Morier
  • Patent number: 7390389
    Abstract: The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.
    Type: Grant
    Filed: August 30, 2002
    Date of Patent: June 24, 2008
    Assignee: Diagnoswiss S.A.
    Inventors: Joel Stephane Rossier, Frédéric Reymond, Philippe Michel
  • Publication number: 20070240986
    Abstract: An electrochemical microfluidic device has one or a plurality of microstructures, such as a microchannel, in which an electrically conductive means is integrated to reduce the ohmic resistance within the microstructure and hence to improve electrochemical measurements particularly when large current densities are involved. The electrically conductive means can be connected as a counter-electrode and can be used to re-generate the product of the reaction occurring at the working electrode. A method of fabricating electrochemical microfluidic devices comprising such an electrically conductive means is also disclosed. The invention may particularly be used in all electrochemical sensor applications where detection is performed in small volumes.
    Type: Application
    Filed: November 11, 2005
    Publication date: October 18, 2007
    Applicants: DIAGNOSWISS S.A., BIOMERIEUX S.A.
    Inventors: Frederic Reymond, Joel Rossier, Patrick Morier
  • Patent number: 7265348
    Abstract: The present invention relates to an apparatus to dispense a sample for subsequent electrospray ionisation (ESI) mass spectrometry (MS) analysis, to a method of fabricating such apparatus and to applications of such apparatus in biological and chemical analysis. The apparatus consists of an electrically non-conductive substrate comprising at least two covered microstructures (generally microchannels) having one extremity formed at the edge of the substrate, one of said microstructures containing the sample to be dispensed into a mass spectrometer by electrospray ionisation and at least a second of said microstructure containing a fluid used as sheath liquid or sheath gas, said at least two microstructures being formed in such a manner that the sample and the sheath liquid/gas come in contact to each other and/or are mixed directly in the Taylor cone of the spray.
    Type: Grant
    Filed: November 7, 2003
    Date of Patent: September 4, 2007
    Assignee: Diagnoswiss S.A.
    Inventors: Joël Stéphane Rossier, Frédéric Reymond
  • Publication number: 20070039835
    Abstract: An electrochemical flow monitoring device comprises a microfluidic system comprising at least one covered microchannel (3) having an inlet (4) and an outlet (5). A pressure difference is applied between the inlet and the outlet of the microfluidic system, for example by changing the relative heights of the inlet (4) and outlet (5), such as to generate a flow of solution within the microchannel (3). The microfluidic system has at least one electrode (8) for monitoring said flow of solution by measuring an electrochemical property of said solution.
    Type: Application
    Filed: September 15, 2004
    Publication date: February 22, 2007
    Applicant: Diagno Swiss S.A.
    Inventors: Joel Rossier, Patrick Morier, Frederic Reymond
  • Publication number: 20060207877
    Abstract: The technology is based on the plasma etching of organic polymer sheets partially protected by a metallic mask. The originality of the process is to pattern the surface properties by the same physical means as the one used for the three dimensional fabrication and simultaneously to this fabrication. Surface properties mean, but are not limited to hydrophobicity hydrophilicity, conductivity, reflectability, rugosity and more precisely the chemical and/or physical state of the surface. It is also possible to generate the desired fonctionalities, for instance carboxylic acid, ester, ether, amid or imide, during the etching process. The patterning of the different properties may be achieved by two different techniques that may be used separately or simultaneously.
    Type: Application
    Filed: April 25, 2006
    Publication date: September 21, 2006
    Inventors: Walter Schmidt, Frederic Reymond, Joel Rossier
  • Patent number: 7098450
    Abstract: The present invention relates to an apparatus and a method of fabricating the apparatus for dispensing a sample for subsequent analysis by mass spectrometry. The apparatus comprises a polymer substrate (1), in which a covered microchannel (3) has been etched, a wall of the microchannel comprising an electrically conductive material (2) for applying a voltage allowing the sample to be sprayed into a mass spectrometer from the open end (4) of the microchannel. The present application also discloses various applications of the apparatus.
    Type: Grant
    Filed: February 13, 2002
    Date of Patent: August 29, 2006
    Assignees: Ecole Polytechnique Federale de Lausanne, DiagnoSwiss S.A.
    Inventors: Joel Stephane Rossier, Frédéric Reymond, Hubert Hugues Girault, Tatiana Rohner, Niels Lion, Véronique Gobry
  • Patent number: 7087181
    Abstract: The technology is based on the anisotropic plasma etching of organic polymer sheets partially protected by a metallic mask. The originality of the process is to pattern the surface properties by the same physical means as the one used for the three dimensional fabrication and simultaneously to this fabrication. Surface properties means, but are not limited to hydrophobicity, hydrophilicity, conductivity, reflectability, rugosity and more precisely the chemical and/or physical state of the surface. It is also possible to generate the desired fonctionalities, for instance carboxylic acid, ester, ether, amid or imid, during the etching process. The patterning of the different properties may be achieved by two different techniques that may be used separately or simultaneously.
    Type: Grant
    Filed: January 30, 2001
    Date of Patent: August 8, 2006
    Assignee: DiagnoSwiss S.A.
    Inventors: Walter Schmidt, Frédéric Reymond, Joël S. Rossier
  • Publication number: 20060141469
    Abstract: The present invention relates to a microfluidic electrochemical sensor apparatus and a method for conducting analytical tests with said apparatus for multi-reactant assays. The apparatus of this invention is a multi-layer body made of at least three layers, the first one being a polymer layer (1) comprising a microstructure (5) with at least one integrated microelectrode (4) and conductive tracks (13) for connection to an external electrochemical unit, the second one being a non-porous material serving to cover said microstructure so as to enable microfluidic manipulations and the third one being a porous layer (2) such as a membrane or a glass frit, said porous layer comprising at least one reagent (3) to be solubilized upon contact with a test solution (7) and reacting with an analyte (6) present in said solution to form a product that is transported along said microstructure so as to enable electrochemical detection of said analyte.
    Type: Application
    Filed: January 14, 2004
    Publication date: June 29, 2006
    Applicant: DiannoSwiss S.S.
    Inventors: Joel Rossier, Frederic Reymond, Patrick Morier
  • Publication number: 20060113463
    Abstract: The present invention relates to an apparatus to dispense a sample for subsequent electrospray ionisation (ESI) mass spectrometry (MS) analysis, to a method of fabricating such apparatus and to applications of such apparatus in biological and chemical analysis. The apparatus consists of an electrically non-conductive substrate comprising at least two covered microstructures (generally microchannels) having one extremity formed at the edge of the substrate, one of said microstructures containing the sample to be dispensed into a mass spectrometer by electrospray ionisation and at least a second of said microstructure containing a fluid used as sheath liquid or sheath gas, said at least two microstrctures being formed in such a manner that the sample and the sheath liquid/gas come in contact to each other and/or are mixed directly in the Taylor cone of the spray.
    Type: Application
    Filed: November 7, 2003
    Publication date: June 1, 2006
    Applicant: DiagnoSwiss S.A.
    Inventors: Joel Rossier, Frederic Reymond
  • Patent number: 7037417
    Abstract: An apparatus for manipulating fluid samples comprises a micro-chip device having first and second covered channels, each channel having openings at both ends. The channels intersect to form a common intersection. Three of the openings of the channels are connected to a multi-port valve to control pressure in the device.
    Type: Grant
    Filed: March 19, 2001
    Date of Patent: May 2, 2006
    Assignees: Ecole Polytechnique Federale de Lausanne, DiagnoSwiss SA
    Inventors: Joel S. Rossier, Frederic Reymond, Hye Jin Lee, Xiaoxia Bai, Hubert H. Girault
  • Publication number: 20040231986
    Abstract: The present invention relates to an apparatus and method for the separation and purification of charged and neutral compounds in an analyte solution by electrophoretic means using a plurality of compartments (1) to contain the analyte solution, an chemical buffering system (2) to fix a pH value or a pH gradient in its portion(s) contacting the analyte solution and an electrical field imposed by electrical means (4) disposed in at least two of said compartments. This invention allows one to differentially separate charged and neutral compounds, to extract the migrating compounds from the chemical buffering system (2), to recover the purified compounds mainly in solution and to collect them in the various compartments (4), preferably at various pI.
    Type: Application
    Filed: February 6, 2004
    Publication date: November 25, 2004
    Inventors: Joel Stephane Rossier, Frederic Reymond, Philippe Michel
  • Publication number: 20040166504
    Abstract: Apparatus method for performing an electrochemical assay or a reaction, using electrical conductivity and/or power in order either to perform a reduction or an oxidation or an ion transfer reaction, or to perform conductimetry and/or impedance measurements, or to generate an electric field in a solution, or to perform any combination of the aforesaid. The apparatus comprises at least one micro-chip (1) possessing a microstructure (2) (for example a microchannel or array or network of microchannels) having a tip end (3) adapted for uptake of a fluid sample into and/or discharge of a fluid sample from said microstructure, a microfluidic connection end (4) and an integral electrode.
    Type: Application
    Filed: December 17, 2003
    Publication date: August 26, 2004
    Inventors: Joel Stephane Rossier, Frederic Reymond, Philippe Michel
  • Publication number: 20040112518
    Abstract: A low temperature method of bonding two polymer sheets (2, 3) without adhesive, at least one of said polymer sheets comprising a microstructure (1) or a network of microstructures, comprises the steps of treating at least a portion of one surface of one of said polymer sheets by using a cold plasma or a laser beam so as to physically activate said portion at low temperature, placing the two polymer sheets in contact, with the activated portion of said one sheet in contact with the other sheet, and subjecting said sheets to pressure and to a temperature below the melting and/or glass transition temperature of either of said polymer sheets, thereby bonding said sheets and forming a sealed micro-structure and/or network of micro-structures. The method is used to fabricate a micro-analytical device for use in biological and/or chemical applications.
    Type: Application
    Filed: October 21, 2003
    Publication date: June 17, 2004
    Inventors: Joel Stephane Rossier, Frederic Reymond, Hubert HuguesGirault
  • Publication number: 20040075050
    Abstract: The present invention relates to an apparatus and a method of fabricating the apparatus for dispensing a sample for subsequent analysis by mass spectrometry. The apparatus comprises a polymer substrate (1), in which a covered microchannel (3) has been etched, a wall of the microchannel comprising an electrically conductive material (2) for applying a voltage allowing the sample to be sprayed into a mass spectrometer from the open end (4) of the microchannel. The present application also discloses various applications of the apparatus.
    Type: Application
    Filed: August 13, 2003
    Publication date: April 22, 2004
    Inventors: Joel Stephane Rossier, Frederic Reymond, Hubert Hugues Girault, Tatiana Rohner, Niels Lion, Veronique Gobry
  • Publication number: 20030104449
    Abstract: A device, method and kit for the electrophoretic separation and purification of charged and neutral compounds in an analyte solution. The device comprises a chamber (1), at least one wall of which is composed of a chemical buffering system (4). A potential difference is applied across the buffering system, resulting in the charged and neutral compounds being differentially separated by extraction of the charged compounds into. the buffering system. The device also comprises means for collecting the separated compounds, preferably in ampholyte-free or buffer-free solution and optionally means for recycling the separated fractions.
    Type: Application
    Filed: October 30, 2002
    Publication date: June 5, 2003
    Inventors: Michel D. Faupel, Hubert H. Girault, Frederic Reymond, Alexandra Ros, Joel Stephane Rossier
  • Publication number: 20030102284
    Abstract: The technology is based on the anisotropic plasma etching of organic polymer sheets partially protected by a metallic mask. The originality of the process is to pattern the surface properties by the same physical means as the one used for the three dimensional fabrication and simultaneously to this fabrication. Surface properties means, but are not limited to hydrophobicity, hydrophilicity, conductivity, reflectability, rugosity and more precisely the chemical and/or physical state of the surface. It is also possible to generate the desired fonctionalities, for instance carboxylic acid, ester, ether, amid or imid, during the etching process. The patterning of the different properties may be achieved by two different techniques that may be used separately or simultaneously.
    Type: Application
    Filed: October 18, 2002
    Publication date: June 5, 2003
    Inventors: Walter Schmidt, Frederic Reymond, Joel S. Rossier
  • Publication number: 20020130044
    Abstract: An apparatus for manipulating fluid samples is described. The apparatus comprises a micro-chip device having at least a first and second covered channel with openings at either end of said first and second covered channel. The first and second covered channels intersect to form a common intersection. In use at least three of the openings to said first and second covered channels are connected to a multi-port valve to control the pressure in the device.
    Type: Application
    Filed: March 19, 2001
    Publication date: September 19, 2002
    Inventors: Joel S. Rossier, Frederic Reymond, Hye Jin Lee, Xiaoxia Bai, Hubert H. Girault