Abstract: Compositions and methods that include stabilized protein drugs are described. In addition, protein drug formulations that are more stable under ambient conditions are described. The formulations include one or more poly amino acid ligands of the protein drug.

Abstract: Compositions and methods that include stabilized protein drugs are described. In addition, protein drug formulations that are more stable under ambient conditions are described. The formulations include one or more poly amino acid ligands of the protein drug.

Abstract: Compositions and methods that include stabilized protein drugs are described. In addition, protein drug formulations that are more stable under ambient conditions are described. The formulations include one or more polyamino acid ligands of the protein drug.

Abstract: The present invention provides methods and systems for developing profiles of a biological system based on the discernment of similarities, differences, and/or correlations between biomolecular components of a plurality of biological samples. Preferably, the method comprises utilizing hierarchical multivariate analysis of spectrometric data at one or more levels of correlation.

Abstract: Reversed-phase materials and a method for producing such materials are disclosed. In making the materials, first a thin layer of an adsorbate comprising reactive amine groups is adsorbed to a support material such as silica, alumina or titania. A portion of the reactive amine groups of said adsorbed coating are then reacted with an amount of a hydrophobic reagent sufficient to effect crosslinking of said coating. At least one remaining reactive amine group, and preferably all of the reactive amine groups of said crosslinked adsorbed coating, are then reacted, preferably in the presence of a proton scavenger, with an amount of a hydrophobic anhydride sufficient to form an amide bond with said reactive amine.

Abstract: Bonded phase chromatography is disclosed wherein an organosilane reacts with a polyamine to form a support matrix which is attached to the inorganic surface of a support structure. In forming an organic polyamine support matrix, a neutral organosilane is bonded to the inorganic surface with the organosilane having a functional group sufficiently reactive so that an organic amine polymer reacts with the functional group to cause covalent bonding of the organic amine polymer to the inorganic surface. By addition of an organic stationary phase group, the organic polyamine support matrix is formed into any predetermined one of a plurality of types of chromatography supports.

Abstract: A pellicular coated support and method for producing such a support are disclosed. The support includes a thin layer of adsorbate that is adsorbed to and cross-linked on an inorganic support material with the thus formed support being particularly well suited for use in liquid chromatography. An inorganic support of silica, alumina or titania has the pellicular coating formed thereon with the coating being an amine that is crosslinked by a crosslinking agent of epoxy resin, bromide or nitro alcohol.

Abstract: An apparatus is disclosed for enzyme detection that is particularly well suited for high performance liquid chromatography. The apparatus is basically a flow-through enzyme detector that is capable of maintaining zero-order reaction kinetics with respect to substrate during enzyme detection and this results in a linear accumulation of product that can be readily measured to provide a dependable readout of detected enzymes. A sample in liquid form that may include one or more enzymes is coupled through a separation column to separate the enzymes, after which a reaction causing substrate is added and the resulting mixture is caused to flow through a reaction chamber, reaction products then being detected with the readout therefrom indicating the detected enzymes. The dimensions of the reaction chamber are dependent upon flow rates and must be selected to allow the mixture to remain in the chamber for a preselected reaction time.

Abstract: A method for analysis of hemoglobin variants by high speed liquid chromatography and, more particularly, by ion-exchange chromatography. Through the use of high flow rates and pressures, the hemoglobin variants can be rapidly forced through the separation column to thus enable rapid analysis. A bonded phase inorganic support column is utilized for separation of the hemoglobin variants with the profiles thereof then being obtained. The bonded phase support preferably includes ion-exchange groups bonded through a carbohydrate intermediate to controlled porosity glass. A pair of different columns may be utilized to separate the hemoglobin variants with one of the columns being an anion-exchanger, and more particularly, a diethylaminoethanol glycophase, controlled porosity glass column, with the other column being a cation-exchanger, and more particularly, a carboxymethyl glycophase, controlled porosity glass column.

Abstract: Chromatographic supports are disclosed as are methods and apparatus for preparing the same. The support includes an inorganic support matrix that is capable of reacting with silanols, a stationary phase, and an intermediate silane coupling agent having a silicon functional group capable of bonding to the support surface and an organic functional group capable of bonding to the stationary phase. The inorganic support matrix is preferably controlled porosity glass or silica while the coupling agent may include a simple carbohydrate, a carbohydrate derivative, or a polymer to which the stationary phase is attached. A plurality of chromatographic supports and stationary phases are disclosed and two methods of bonding of stationary phases to supports are also disclosed. In addition, apparatus including a fluidized bed is also disclosed to prepare supports.

Abstract: A carbohydrate bonded support for use in a chromatographic system, and a method for preparation of such a support is disclosed herein. An inorganic support has a thin layer of carbohydrate or carbohydrate derivative covalently linked to the surface so that the thus formed support has excellent mechanical stability as well as the ability to avoid adsorbing or denaturing sensitive biological compounds, the carbohydrate bonded support thus combining the advantages of known supports without also incorporating the disadvantages thereof.