Abstract: The present invention provides methods for the screening and identification of agents from a large library of molecular structures that can alter the cleavage of amyloid precursor protein (AP). Agents identified by the methods of the present invention that modify the cleavage of APP can be used in the treatment and prevention of Alzheimer's disease. The methods select for and identify effector agents that bind to APP causing a structural change in the structure of APP in such a way that the efficiency of the cleavage of a secretase is modulated. Further, the methods are carried out in an in vivo system that provides for physiological conditions similar or identical to conditions for APP processing. Agents can be selected for their ability to cause a decrease in the amount of B-secretase or ?-secretase cleavage of APP, or for an increase in a-secretase cleavage of APP.

Abstract: The present invention provides methods for the screening and identification of agents from a large library of molecular structures that can alter the cleavage of a membrane protein of interest. Agents identified by the methods of the present invention that modify the cleavage of the membrane protein can be used in the treatment and prevention of diseases such as inflammation, diabetes, cancer, Alzheimer's disease, Parkinson's disease, and the like. The methods select for and identify effector agents that bind to the membrane protein of interest causing a structural change in the structure of the membrane protein in such a way that the efficiency of the cleavage of a secretase is modulated. Further, the methods are carried out in an in vivo system that provides for physiological conditions similar or identical to conditions for membrane protein processing. Agents can be selected for their ability to cause a decrease or increase the amount of secretase cleavage of the membrane protein.

Abstract: The present invention provides libraries expressing peptide libraries on the extracellular cell surface of host cells and methods for identifying peptides that bind extracellular target molecules under the physiological conditions encountered in biological fluids and secretions. The present invention is also directed to vectors for expressing gene fusion proteins and for targeting those fusion proteins to the extracellular cell surface.

Abstract: A method for removing one or more fluorinated alkenes from a fluid comprises the step of contacting the fluid with an N-, S-, or P-containing nucleophile for a time sufficient to form an N-, S-, or P-containing nucleophile-fluoroalkene adduct. The nucleophile, and therefore the adduct, can be covalently bonded, coated or sorbed to a particulate support which can be enmeshed in a porous, fibrous web. In a preferred embodiment the fluorinated alkene can have the formula wherein A=F or Rf; X=H, F, Cl, Rf, or YRf; Z= H, F, Cl, or Rf; and Y=O, N, or S; with the proviso that at least one A=F, and at most one of Z and X is H; wherein each Rf is independently selected from the group consisting of highly fluorinated or perfluorinated alkyl groups; and a combination of any two Rf groups can be linked to form a cyclic structure. The Rf alkyl chains may contain from 1-20 carbon atoms, with 1-12 carbon atoms preferred.

Abstract: A method for removing one or more fluorinated alkenes from a fluid comprises the step of contacting the fluid with an N-, S-, or P-containing nucleophile for a time sufficient to form an N-, S-, or P-containing nucleophile-fluoroalkene adduct. The nucleophile, and therefore the adduct, can be covalently bonded, coated or sorbed to a particulate support which can be enmeshed in a porous, fibrous web. In a preferred embodiment the fluorinated alkene can have the formula wherein A=F or Rf; X=H, F, Cl, Rf, or YRf; Z=H, F, Cl, or Rf; and Y=O, N, or S; with the proviso that at least one A=F, and at most one of Z and X is H; wherein each Rf is independently selected from the group consisting of highly fluorinated or perfluorinated alkyl groups; and a combination of any two Rf groups can be linked to form a cyclic structure. The Rf alkyl chains may contain from 1-20 carbon atoms, with 1-12 carbon atoms preferred.

Abstract: A method for removing one or more fluorinated alkenes from a fluid comprises the step of contacting the fluid with an N-, S-, or P-containing nucleophile for a time sufficient to form an N-, S-, or P-containing nucleophile-fluoroalkene adduct. The nucleophile, and therefore the adduct, can be covalently bonded, coated or sorbed to a particulate support which can be enmeshed in a porous, fibrous web.

Abstract: A method for removing one or more fluorinated alkenes from a fluid comprises the step of contacting the fluid with an N-, S-, or P-containing nucleophile for a time sufficient to form an N-, S-, or P-containing nucleophile-fluoroalkene adduct. The nucleophile, and therefore the adduct, can be covalently bonded, coated or sorbed to a particulate support which can be enmeshed in a porous, fibrous web.

Abstract: A method for removing one or more fluorinated alkenes from a fluid comprises the step of contacting the fluid with an N-, S-, or P-containing nucleophile for a time sufficient to form an N-, S-, or P-containing nucleophile-fluoroalkene adduct. The nucleophile, and therefore the adduct, can be covalently bonded, coated or sorbed to a particulate support which can be enmeshed in a porous, fibrous web. In a preferred embodiment the fluorinated alkene can have the formula ##STR1## wherein A=F or R.sub.f ; X=H, F, Cl, R.sub.f, or YR.sub.f ; Z=H, F, Cl, or R.sub.f ; and Y=O, N, or S; with the proviso that at least one A=F, and at most one of Z and X is H; wherein each R.sub.f is independently selected from the group consisting of highly fluorinated or perfluorinated alkyl groups; and a combination of any two R.sub.f groups can be linked to form a cyclic structure. The R.sub.f alkyl chains may contain from 1-20 carbon atoms, with 1-12 carbon atoms preferred. The R.sub.