Patents by Inventor Frederick J. Darfler

Frederick J. Darfler has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Protein-free culture medium which promotes hybridoma growth
    Patent number: 5045468
    Abstract: A chemically-defined, stable, protein-free medium has been devised which is capable of supporting the growth of lymphoid cells, particularly hybridomas, even in anchorage-independent cultures. Doubling rates are close to those obtained with serum-supplemented media. The medium preferably comprises a nonproteinaceous organo-iron compound, especially a nitroprusside salt, an iron chelator such as EDTA, and a selenium compound, such as selenium dioxide or sodium selenite.
    Type: Grant
    Filed: August 15, 1989
    Date of Patent: September 3, 1991
    Assignee: Cell Enterprises, Inc.
    Inventor: Frederick J. Darfler
  • Cell culture medium with antioxidant
    Patent number: 4927762
    Abstract: Cells grown in chemically defined (serum free) media are protected against endogenous oxidizing agents by a nontoxic antioxidant with free thiol groups, at cell densities less than about 10.sup.5 cells/ml, especially DL-penicillamine, N-acetylcysteine, mercaptoproprionic acid, 2-mercaptoethanesulfonic acid, and thiolactate. These agensts are useful in the maintenance and growth of hybridomas in such media.
    Type: Grant
    Filed: November 30, 1988
    Date of Patent: May 22, 1990
    Assignee: Cell Enterprises, Inc.
    Inventor: Frederick J. Darfler