Abstract: High throughput compound profiling systems, and related devices and sub-systems that can be used to perform various compound profiling processes are provided. These systems typically include work perimeters that are organized for optimum efficiency and processing accuracy. Further, these systems are readily adaptable for performing a wide array of assays, as many different system components are easily incorporated or interchangeable in a particular system. System components that are provided by the invention include cell culture dissociators, which can be used, e.g., to effect cell wetting, dissociation, and/or agitation applications. In some embodiments, these cell culture dissociators are included as components of automated cell culture passaging stations. Dispensing devices that permit on-the-fly fluid temperature regulation are also provided. In addition, various compound profiling methods, cell dissociation methods, uniform cell concentration dispensing methods, among other processes, are also provided.

Abstract: The present invention relates to novel SH3 domain binding protein, referred to herein a DEF polypeptides. The DEF polypeptides comprise several motifs including a src SH3 consensus binding sequence, four ankyrin repeats, one zinc finger domain and six copies of a proline-rich tandem repeat. DEF polypeptides may function as mediators of SH3 domain-dependent signal transduction pathways and, thus may mediate multiple signaling events such as cellular gene expression, cytoskeletal architecture, protein trafficking and endocytosis, cell adhesion, migration, proliferation and differentiation. Described herein are isolated and antisense nucleic acids molecules, recombinant expression vectors, host cells and non-human transgenic animals containing an insertion or a disruption of the DEF gene.

Abstract: Apparatus is disclosed for the simultaneous adjustment and setting of a pair of combine screens. A tie bar arrangement is provided, interconnecting at least a pair of side-by-side sieve screens. An operating handle is pivotally mounted to the frame and is pivotally connected to the tie bars and extends outwardly of the apparatus for ease of use. A scale or gauge is provided to indicate the amount of handle movement and therefore, screen opening.

Abstract: A thermostat for cyclically regulating actual ambient temperature, Ta, to either of two values, T1 and T2, for selectable durations. A switch allows T1 to be selected and deselected and a sensor detects Ta, while a controller is connected to the switch and the sensor. The controller is capable of defining T1 and T2 values. The controller further has a memory to store a cycle period duration, a detector to detect when the switch selects or deselects T1, and a timer which establishes T1 duration based on the time between T1 selection and deselection, and establishes T2 duration as at least a portion of the remainder of the cycle period. The controller automatically cyclically repeats the established T1 and T2 durations during each cycle period until T1 is again selected by the switch. A signal generator in the controller generates a control signal corresponding to the difference between Ta and T1 or T2 during their respective durations.