Patents by Inventor Frederick Pfleger
Frederick Pfleger has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20250163478Abstract: Described is a recombinant expression vector that enables a cell transformed to contain and express the vector to use levulinic acid as a carbon source, thereby converting levulnic acid into 2-butanne. Also described are genetically modified cells transformed to contain and express the vector and methods of using the cells to produce 2-butanone from a medium containing levulinic acid.Type: ApplicationFiled: September 13, 2024Publication date: May 22, 2025Applicant: Wisconsin Alumni Research FoundationInventors: Brian Frederick Pfleger, Jacqueline Marie Rand, Christopher Robert Mehrer, Matthew Ryan Incha
-
Patent number: 12116609Abstract: Described is a recombinant expression vector that enables a cell transformed to contain and express the vector to use levulinic acid as a carbon source, thereby converting levulnic acid into 2-butanne. Also described are genetically modified cells transformed to contain and express the vector and methods of using the cells to produce 2-butanone from a medium containing levulinic acid.Type: GrantFiled: January 27, 2021Date of Patent: October 15, 2024Assignee: Wisconsin Alumni Research FoundationInventors: Brian Frederick Pfleger, Jacqueline Marie Rand, Christopher Robert Mehrer, Matthew Ryan Incha
-
Publication number: 20210155961Abstract: Described is a recombinant expression vector that enables a cell transformed to contain and express the vector to use levulinic acid as a carbon source, thereby converting levulnic acid into 2-butanne. Also described are genetically modified cells transformed to contain and express the vector and methods of using the cells to produce 2-butanone from a medium containing levulinic acid.Type: ApplicationFiled: January 27, 2021Publication date: May 27, 2021Inventors: Brian Frederick Pfleger, Jacqueline Marie Rand, Christopher Robert Mehrer, Matthew Ryan Incha
-
Patent number: 10982240Abstract: Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.Type: GrantFiled: March 8, 2018Date of Patent: April 20, 2021Assignee: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Brian Frederick Pfleger, Daniel E. Agnew
-
Patent number: 10934563Abstract: Described is a recombinant expression vector that enables a cell transformed to contain and express the vector to use levulinic acid as a carbon source, thereby converting levulnic acid into 2-butanne. Also described are genetically modified cells transformed to contain and express the vector and methods of using the cells to produce 2-butanone from a medium containing levulinic acid.Type: GrantFiled: September 19, 2018Date of Patent: March 2, 2021Assignee: Wisconsin Alumni Research FoundationInventors: Brian Frederick Pfleger, Jacqueline Marie Rand, Christopher Robert Mehrer, Matthew Ryan Incha
-
Publication number: 20190085362Abstract: Described is a recombinant expression vector that enables a cell transformed to contain and express the vector to use levulinic acid as a carbon source, thereby converting levulnic acid into 2-butanne. Also described are genetically modified cells transformed to contain and express the vector and methods of using the cells to produce 2-butanone from a medium containing levulinic acid.Type: ApplicationFiled: September 19, 2018Publication date: March 21, 2019Inventors: Brian Frederick Pfleger, Jacqueline Marie Rand, Christopher Robert Mehrer, Matthew Ryan Incha
-
Publication number: 20180201961Abstract: Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.Type: ApplicationFiled: March 8, 2018Publication date: July 19, 2018Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: Brian Frederick Pfleger, Daniel E. Agnew
-
Patent number: 8846354Abstract: Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.Type: GrantFiled: March 7, 2014Date of Patent: September 30, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Brian Frederick Pfleger, Matthew Brett Begemann
-
Patent number: 8846329Abstract: Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.Type: GrantFiled: March 7, 2014Date of Patent: September 30, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Brian Frederick Pfleger, Matthew Brett Begemann
-
Patent number: 8715973Abstract: Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.Type: GrantFiled: March 13, 2013Date of Patent: May 6, 2014Assignee: Wisconsin Alumni Research FoundationInventors: Brian Frederick Pfleger, Matthew Brett Begemann
-
Publication number: 20140073022Abstract: Cells and methods for producing polyhydroxyalkanoates. The cells comprise one or more recombinant genes selected from an R-specific enoyl-CoA hydratase gene, a PHA polymerase gene, a thioesterase gene, and an acyl-CoA-synthetase gene. The cells further have one or more genes functionally deleted. The functionally deleted genes include such genes as an enoyl-CoA hydratase gene, a 3-hydroxyacyl-CoA dehydrogenase, and a 3-ketoacyl-CoA thiolase gene. The recombinant cells are capable of using producing polyhydroxyalkanoates with a high proportion of monomers having the same carbon length from non-lipid substrates, such as carbohydrates.Type: ApplicationFiled: March 15, 2013Publication date: March 13, 2014Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATIONInventors: BRIAN FREDERICK PFLEGER, DANIEL E. AGNEW
-
Patent number: 6289656Abstract: A packaging machine for enclosing items in a package delivers individual items, such as medicine, through a feeder that disposes the item between two strips of packaging material which are then joined together around the item by a closure member thus sealing the item in an individual package. The packaging machine demarcates upper and lower boundaries about the item such that the enclosed item may be separated from a continuously produced strand of packages. The packaging machine includes a selectively operable hole punch portion disposed adjacent the strand of packages for providing a hole in a portion of each package. The hole punch portion includes a punch driving member which is disengable responsive to unexpected resistance encountered by the punch. A cutter/stacker portion adjacent the strand of separable packages separates the strand into individual packages and stacks the packages in a removable magazine.Type: GrantFiled: July 12, 2000Date of Patent: September 18, 2001Assignees: McKesson Automated Healthcare, Inc., FP Developments, Inc.Inventors: Manoj K. Wangu, Frederick Pfleger, Paul J. Eachus, Michele L. Porcelli, Jr.