Abstract: Human milk oligosaccharides (HMOs) may be used e.g. as functional ingredients in infant nutrition, medical nutrition, functional foods and animal feed. There is still a need of improved means of producing HMOs. The present invention provides genetically modified microorganisms for the improved production of HMOs and HMO production methods using the same. The microorganisms of the invention may have one or more yield-enhancing modifications, including an inducible lysis system, which allows for the easy extraction of intracellular and extracellular HMOs, lactose permease mutants, which may increase intracellular lactose levels, or chaperones, which may increase intracellular availability of key enzymes for the production of HMOs.

Abstract: Human milk oligosaccharides (HMOs) may be used e.g. as functional ingredients in infant nutrition, medical nutrition, functional foods and animal feed. There is still a need of improved means of producing HMOs. The present invention provides genetically modified microorganisms for the improved production of HMOs and HMO production methods using the same. The microorganisms of the invention may have one or more yield-enhancing modifications, including an inducible lysis system, which allows for the easy extraction of intracellular and extracellular HMOs, lactose permease mutants, which may increase intracellular lactose levels, or chaperones, which may increase intracellular availability of key enzymes for the production of HMOs.

Abstract: The invention relates to the factor RecA from bacillus licheniformis DSM 13 (SEQ ID NO: 2), along with the associated gene recA (SEQ ID NO: 1), including related proteins and genes thereof, such as the variant indicated under SEQ ID NOS: 31 and 32, among others. According to the invention, gene recA is used for constructing gram-positive bacterial safety stems for biotechnological production, among other things, by inactivating the same in the respective stems. In a special embodiment, said stems are provided with additional functional deletions in phase-IV sporulation genes, preferably in gene SpoIV (in Bacillus licheniformis), gene yqfD (in B. subtilis), or the respective gene that is homologous thereto if said stems are naturally able to form spores. Furthermore, the inventive RecA represents a protein which can be used in molecular biological assays or for modulating the molecular biological activities of cells, especially in connection with DNA polymerization or recombination processes.

Abstract: A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of the enzyme glucose dehydrogenase and a new DNA sequence, recombinant DNA vector, and transformed microrganism therefor.

Abstract: A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of the enzyme glucose dehydrogenase, and a new DNA sequence, recombinant DNA vector, and transformed microorganism therefor.