Abstract: A composite including poly(hydroxyalkanoate); and graphene. Also disclosed are methods of making articles.

Abstract: A composite including poly(hydroxyalkanoate); and graphene. Also disclosed are methods of making articles.

Abstract: The present invention describes a number of different microorganisms that have been genetically-engineered to optimize ethanol production. The present invention also describes methods of using such microorganisms to efficiently make ethanol.

Abstract: Systems and methods are provided for analyzing particulates. A liquid having a plurality of particulates substantially linearly ordered in a streamline can be externally controlled to provide flow in first and second directions, where, generally, the first direction is opposite to the second direction. A target particulate can be measured from the plurality of particulates at or near a measurement area while the liquid flows in the first flow direction. The flow direction can be reversed and measured at the measurement area while flowing in the second direction. The particulates substantially retain the same linear order during at least one cycle, a cycle being defined by movement in the first direction followed by movement in the second direction.

Abstract: It has surprisingly been discovered that it is possible to use enzymes in deep eutectic solvents (DES). DES's are mixtures of a nitrogen salt or a metal salt and a strong hydrogen bond donor that can be mixed in proportions that form a eutectic point.

Abstract: A continuous flow bioreactor system that includes a bioreactor, an optional post-bioreactor preparation chamber, a cell sorter, and an optional pre-bioreactor preparation chamber in a closed loop, useful for enriching a heterogeneous cell population growing in the bioreactor with an isolated subpopulation of cells.

Abstract: The present invention describes a number of different microorganisms that have been genetically-engineered to optimize ethanol production. The present invention also describes methods of using such microorganisms to efficiently make ethanol.

Abstract: An improved E. coli for carotenoid production comprising exogenous nucleic acids for expressing a carotenoid that the E. coli requires as necessary condition for the E. coli to reproduce. Some E. coli embodiments have diminished or abrogated expression of a gene in the group consisting of ldhA, frdA, poxB, pta, adhE, pykF, zwf, and maeB.

Abstract: It has surprisingly been discovered that it is possible to use enzymes in deep eutectic solvents (DES). DES's are mixtures of a nitrogen salt or a metal salt and a strong hydrogen bond donor that can be mixed in proportions that form a eutectic point.

Abstract: A continuous flow bioreactor system that includes a bioreactor, an optional post-bioreactor preparation chamber, a cell sorter, and an optional pre-bioreactor preparation chamber in a closed loop, useful for enriching a heterogeneous cell population growing in the bioreactor with an isolated subpopulation of cells.

Abstract: Systems and methods are provided for analyzing particulates. A liquid having a plurality of particulates substantially linearly ordered in a streamline can be externally controlled to provide flow in first and second directions, where, generally, the first direction is opposite to the second direction. A target particulate can be measured from the plurality of particulates at or near a measurement area while the liquid flows in the first flow direction. The flow direction can be reversed and measured at the measurement area while flowing in the second direction. The particulates substantially retain the same linear order during at least one cycle, a cycle being defined by movement in the first direction followed by movement in the second direction.

Abstract: A system identifies or screens one or more biological materials. The system includes at least one bioreactor adapted to provide biological samples and an automated sample handler comprising at least one bi-directional pump to transport biological samples, reagents and fluids, at least one reaction chamber with at least two regions separated by a semi-permeable membrane and fitted with a thermoelectric module for cooling and heating the chamber. At least one multi-positional valve directs transport of the biological samples, reagents and fluids into and out of the reaction chamber and a micro-processor to control operations of the sample handler. A detector is used to identify the biological material in the samples and a sample repository collects the identified biological samples.

Abstract: Biopolymers and biopolymer blends, including block copolymers, prepared via enzyme-mediated catalysis preferably in a microorganism host in which the reaction conditions are selected to produce biopolymers and biopolymer blends having particular chemical compositions and microstructures.

Abstract: Transgenic yeast strains and methods for producing polyhydroxyalkanoate (PHA). A genetically engineered Pseudomonas oleovorans polyhydroxyalkanoate (PHA) polymerase was expressed in the cytosol of some wild type yeast strains, the pex5 mutants and a fox3 mutant. The composition of the PHA was influenced by the genetic background of the yeast host, the monomer specificity of the polymerase, the cellular compartment in which the polymerase was active, and the substrate supplied in the medium. The culture strategies and further metabolic pathway engineering technologies were provided. This platform provides a basis for controlling the composition and thus the properties of the synthesized PHA.

Abstract: A continuous flow bioreactor system that includes a bioreactor, an optional post-bioreactor preparation chamber, a cell sorter, and an optional pre-bioreactor preparation chamber in a closed loop, useful for enriching a heterogeneous cell population growing in the bioreactor with an isolated subpopulation of cells.

Abstract: Biopolymers and biopolymer blends, including block copolymers, prepared via enzyme-mediated catalysis preferably in a microorganism host in which the reaction conditions are selected to produce biopolymers and biopolymer blends having particular chemical compositions and microstructures.

Abstract: Biopolymers and biopolymer blends, including block copolymers, prepared via enzyme-mediated catalysis preferably in a microorganism host in which the reaction conditions are selected to produce biopolymers and biopolymer blends having particular chemical compositions and microstructures.

Abstract: For direct and on-line study of the physiological states of cell cultures, a robust flow injection system has been designed and interfaced with flow cytometry (FI-FCM). The core of the flow injection system includes a microchamber designed for sample processing. The design of the microchamber allows not only an accurate on-line dilution but also on-line cell fixation, staining, and washing. The flow injection part of the system was tested by monitoring the optical density of a growing E.coli culture on-line using a spectrophotometer. The entire growth curve, from lag phase to stationary phase, was obtained with frequent sampling. The system is thus particularly useful since it operates automatically without direct operator supervision for extended time periods.

Abstract: Biopolymers and biopolymer blends, including block copolymers, prepared via enzyme-mediated catalysis preferably in a microorganism host in which the reaction conditions are selected to produce biopolymers and biopolymer blends having particular chemical compositions and microstructures.

Abstract: Novel transgenic microorganisms capable of biosynthesis of polyhydroxyalkanoate (PHA). Heterologous enzymes involved in PHA biosynthesis, particularly PHA polymerase and acetoacetyl-CoA reductase, are expressed in transgenic prokaryotic and eukaryotic microorganisms. PHA biosynthesis can occur either anaerobically or aerobically.