Patents by Inventor Fu-Kai HSIEH

Fu-Kai HSIEH has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • GENE EDITING SYSTEMS COMPRISING A CRISPR NUCLEASE AND USES THEREOF
    Publication number: 20240102007
    Abstract: A gene editing system comprising: (a) a Type V CRISPR nuclease polypeptide or a first nucleic acid encoding the Type V CRISPR nuclease polypeptide; (b) a reverse transcriptase (RT) polypeptide or a second nucleic acid encoding the RT polypeptide; (c) a guide RNA (gRNA) or a third nucleic acid encoding the gRNA, wherein the gRNA comprises one or more binding sites recognizable by the Type V CRISPR nuclease (CRISPR nuclease binding sites) and a spacer sequence specific to a target sequence within a genomic site of interest, the target sequence being adjacent to a protospacer adjacent motif (PAM); and (d) a reverse transcription donor RNA (RT donor RNA) or a fourth nucleic acid encoding the RT donor RNA, wherein the RT donor RNA comprises a primer binding site (PBS) and a template sequence.
    Type: Application
    Filed: June 1, 2022
    Publication date: March 28, 2024
    Applicant: Arbor Biotechnologies, Inc.
    Inventors: David A. Scott, Noah Michael Jakimo, Pratyusha Hunnewell, Fu-Kai Hsieh
  • GENE EDITING SYSTEMS COMPRISING A CRISPR NUCLEASE AND USES THEREOF
    Publication number: 20230023791
    Abstract: A gene editing system comprising: (a) a Type V CRISPR nuclease polypeptide or a first nucleic acid encoding the Type V CRISPR nuclease polypeptide; (b) a reverse transcriptase (RT) polypeptide or a second nucleic acid encoding the RT polypeptide; (c) a guide RNA (gRNA) or a third nucleic acid encoding the gRNA, wherein the gRNA comprises one or more binding sites recognizable by the Type V CRISPR nuclease (CRISPR nuclease binding sites) and a spacer sequence specific to a target sequence within a genomic site of interest, the target sequence being adjacent to a protospacer adjacent motif (PAM); and (d) a reverse transcription donor RNA (RT donor RNA) or a fourth nucleic acid encoding the RT donor RNA, wherein the RT donor RNA comprises a primer binding site (PBS) and a template sequence.
    Type: Application
    Filed: June 1, 2022
    Publication date: January 26, 2023
    Inventors: David A. SCOTT, Noah Michael JAKIMO, Pratyusha HUNNEWELL, Fu-Kai HSIEH