Abstract: The invention provides a bacterial cellulose-based biosensor, including bacterial cellulose (BC) and a cell presenting a cellulose-binding module CBM2a on the surface. The cell is attached to BC through CBM2a. The cell expresses CBM2a by using pETDuet-tac as a vector. The pETDuet-tac is obtained by replacing two T7 promoters on the vector pETDuet by tac promoters, that is, an upstream first tac promoter and a downstream second tac promoter. The pETDuet-tac includes a gene encoding a fluorescent protein downstream of the first tac promoter and a gene encoding CBM2a presented on the surface downstream of the second tac promoter. By the BC-based biosensor, efficient and specific immobilization of cells on the BC matrix is enabled, the biological activity of cells is maintained, the fluorescence signal output is enhanced, and sufficient pores are provided for the entry and exit of a detected substance, thereby significantly improving the detection sensitivity.

Abstract: Disclosed is oleaginous yeast and its application, belonging to the technical field of microorganisms. The oleaginous yeast provided by the disclosure has been deposited in China Center for Type Culture Collection on May 21, 2020, with an accession number of CCTCC NO: M 2020139. The oleaginous yeast provided by the disclosure can use a hydrolysate of cheap lignocellulose biomass as the substrate. Xylose and glucose in the hydrolysate are simultaneously utilized to produce oil, so the sugar utilization efficiency and production intensity are enhanced. After 8 days of fed-batch fermentation, the oil yield can reach 31.33 g/L, and the oil content can reach 60.83%. The waste resources can be reutilized, and the production cost is reduced, so the oleaginous yeast has wide application prospects.

Abstract: The present invention discloses a method for co-production and processing of biological energy sources by oil crops, and belongs to the technical fields of oleochemical industry and biomass chemical industry. The method comprises the following steps: husking and drying oil crop seeds to obtain husks of which the moisture content is 5%-12% and oil seeds of which the moisture content is 30%-55%; squeezing and extracting the obtained oil seeds to obtain vegetable oil and oil seed meal; performing esterification reaction on the vegetable oil and alcohol to be separated to obtain bio-diesel and crude glycerol; performing cooking treatment on the husks and/or the oil seed meal in the crude glycerol to be separated to obtain oil crude cellulose and glycerol treating liquid; performing microbial fermentation or anaerobic digestion on the oil crude cellulose and the glycerol treating liquid respectively to obtain biological energy sources or bio-based chemicals.

Abstract: The present invention discloses a method for co-production and processing of biological energy sources by oil crops, and belongs to the technical fields of oleochemical industry and biomass chemical industry. The method comprises the following steps: husking and drying oil crop seeds to obtain husks of which the moisture content is 5%-12% and oil seeds of which the moisture content is 30%-55%; squeezing and extracting the obtained oil seeds to obtain vegetable oil and oil seed meal; performing esterification reaction on the vegetable oil and alcohol to be separated to obtain bio-diesel and crude glycerol; performing cooking treatment on the husks and/or the oil seed meal in the crude glycerol to be separated to obtain oil crude cellulose and glycerol treating liquid; performing microbial fermentation or anaerobic digestion on the oil crude cellulose and the glycerol treating liquid respectively to obtain biological energy sources or bio-based chemicals.