Abstract: A method for detecting a single nucleotide polymorphism in nucleic acids, characterized in that the method includes mixing (A) a nucleic acid probe comprising a nucleotide sequence complementarily hybridizable to an evaluation subject nucleic acid/an antisense strand thereof containing at least one single nucleotide polymorphism, and tagged with a nucleotide sequence of a hairpin structure having a bulge at a 5?-terminal thereof, wherein a guanine residue is introduced at an adjoining position of the bulge, and wherein a naphthyridine derivative compound is immobilized to the bulge; and (B) the evaluation subject nucleic acids; and detecting a signal ascribed to the naphthyridine derivative compound when the above nucleic acid probe and the above evaluation subject nucleic acids are hybridized, thereby evaluating the above single nucleotide polymorphism.

Abstract: A method is provided which enables quick, convenient, inexpensive, and high sensitivity confirmation of nucleic acid amplification after a nucleic acid amplification reaction. The DNA fragment in accordance with the present invention is a single-stranded DNA fragment containing a hairpin structure which in turn contains a bulge, wherein the DNA fragment is used as being attached to the 5? end of a primer used in nucleic acid amplification. The nucleic acid amplification confirmation method in accordance with the present invention quantifies a hairpin primer containing the DNA fragment at its 5? end by using bulge-binding fluorescent molecules after carrying out PCR or like nucleic acid amplification reaction using the hairpin primer. SNPs are detected quickly and conveniently at low cost and with high sensitivity by applying the nucleic acid amplification confirmation method, for example, to allele specific PCR.

Abstract: A method for detecting a single nucleotide polymorphism in nucleic acids, characterized in that the method includes mixing (A) a nucleic acid probe comprising a nucleotide sequence complementarily hybridizable to an evaluation subject nucleic acid/an antisense strand thereof containing at least one single nucleotide polymorphism, and tagged with a nucleotide sequence of a hairpin structure having a bulge at a 5?-terminal thereof, wherein a guanine residue is introduced at an adjoining position of the bulge, and wherein a naphthyridine derivative compound is immobilized to the bulge; and (B) the evaluation subject nucleic acids; and detecting a signal ascribed to the naphthyridine derivative compound when the above nucleic acid probe and the above evaluation subject nucleic acids are hybridized, thereby evaluating the above single nucleotide polymorphism.

Abstract: A method is provided which enables quick, convenient, inexpensive, and high sensitivity confirmation of nucleic acid amplification after a nucleic acid amplification reaction. The DNA fragment in accordance with the present invention is a single-stranded DNA fragment containing a hairpin structure which in turn contains a bulge, wherein the DNA fragment is used as being attached to the 5? end of a primer used in nucleic acid amplification. The nucleic acid amplification confirmation method in accordance with the present invention quantifies a hairpin primer containing the DNA fragment at its 5? end by using bulge-binding fluorescent molecules after carrying out PCR or like nucleic acid amplification reaction using the hairpin primer. SNPs are detected quickly and conveniently at low cost and with high sensitivity by applying the nucleic acid amplification confirmation method, for example, to allele specific PCR.