Abstract: There are provided a cell aggregate that can be used as a cell preparation in regenerative medicine, maintains the original characteristics of cells, and has high safety, and a method for simply and safely maintaining the cell aggregate size, the cell characteristics and viability suitable for use as a cell preparation in regenerative medicine. According to an aspect of the present invention, a cell aggregate maintaining method is a method including a maintenance process of maintaining a liquid containing a plurality of the cell aggregates at a temperature which is lower than a culture temperature of the cells and at which the liquid is not frozen.

Abstract: Provided is a method for regenerating a liquid to be treated, the method includes bringing a lactic acid adsorbent that contains a Mg—Al-based layered double hydroxide having Mg2+ and Al3+ as constituent metals, and an ammonia adsorbent having L-type zeolite, into contact with the liquid to be treated that contains lactic acid and ammonia, to remove lactic acid and ammonia in the liquid to be treated.

Abstract: A lactic acid adsorbent includes at least one of a layered double hydroxide that contains multiple metal hydroxide layers and also contains anions and water molecules held between the metal hydroxide layers, or a layered double oxide that is an oxide of a layered double hydroxide. The metal hydroxide layers contain divalent metal ions M2+ and trivalent metal ions M3+, mole ratio (M2+/M3+) of the divalent metal ions M2+ to the trivalent metal ions M3+ in a layered double hydroxide is 1.9 to 3.6, and the mole ratio in a layered double oxide is 1.8 to 3.6.

Abstract: A lactic acid adsorbent includes a layered double hydroxide that contains multiple metal hydroxide layers and also contains anions and water molecules held between the metal hydroxide layers. The anions include (i) an amino acid such as glutamine, (ii) a dipeptide constituted by one or two kinds of amino acids such as glutamine, (iii) a vitamin such as ascorbic acid 2-phosphate, (iv) a pH buffer such as MES, (v) a glucose metabolite such as pyruvic acid, or (vi) inorganic ions selected from a group including NO3? and Cl?.

Abstract: The ammonia adsorbent contains at least one substance selected from the group consisting of L-type zeolite, ferrierite, ZSM-5 type zeolite, strongly acidic cation exchange resin and Prussian blue type complex.

Abstract: The lactic acid adsorbent contains at least one substance selected from the group consisting of layered double hydroxide, layered double oxide, weakly basic anion exchange resin having a styrene-bound dimethylamino group, and a strongly basic anion exchange resin.

Abstract: A cell culture method includes culturing a cell in a culture solution that satisfies at least one of condition (I) or condition (II) below. Condition (I): lactic acid concentration in the culture solution being lower than 7.5 mM. Condition (II): ammonia concentration in the culture solution being lower than 2.5 mM.

Abstract: A method of culturing cells includes placing a cell suspension including i) one of more types of adhesion molecules selected from the group consisting of fragments of laminin molecules, fragments of a basement membrane matrix mixture, and a complete basement membrane matrix mixture and ii) kidney cells on a culture surface of a substrate; and culturing the kidney cells on the substrate to form a confluent monolayer of the cells.

Abstract: A cell support composite includes: a substrate formed of an artificial material; a laminin molecule or a fragment thereof which adheres to at least a part of the substrate; and a renal tubule epithelial cell or a renal tubule epithelial-like cell which is a culture cell attached to the substrate via the laminin molecule or the fragment thereof.

Abstract: A method of culturing cells includes culturing the cell in a cell suspension under repetitive cycles of stirring and rest while keeping the cell non-adherent to a culture vessel, so as to form at least one aggregate of the cells.

Abstract: A cell cultivation method includes cultivating dedifferentiated kidney cells in a state of being non-adherent to a culture vessel for a period of 5 days or longer, forming aggregates of the kidney cells during the cultivation period, then cultivating the kidney cells in a state of having formed aggregates, during a portion of the period, and thereby restoring the physiological functions of the kidney cells.

Abstract: A cell support composite includes: a substrate formed of an artificial material; a laminin molecule or a fragment thereof which adheres to at least a part of the substrate; and a renal tubule epithelial cell or a renal tubule epithelial-like cell which is a culture cell attached to the substrate via the laminin molecule or the fragment thereof.

Abstract: A colony (cell mass) proliferated under the undifferentiated state is obtained by using a carrier for cell culture in which two or more of a concavity having a porous body in a surface are arranged on a substrate surface in the form of a matrix, inoculating an undifferentiated cell on at least one concavity of the carrier for culture and carrying out culture.

Abstract: A culture substrate made of ceramics of any one or more of titania, alumina, zirconia, yttria, and carbon is used where minute pores having a pore diameter of from 0.1 ?m to 10 ?m are provided at least for a place to which a cell is seeded, and an undifferentiated cell is seeded to at least one place of a surface of the culture substrate, whereby a culture substrate and a culture method for efficiently culturing the undifferentiated cell to be formed into a 3-dimensional cell block shape without causing immune rejection, and an undifferentiated cultured cell.

Abstract: The present invention relates to a cell culture support for culturing mesenchymal stem cells, which includes en upper surface including a plurality of wells, in which the upper surface has a root mean square roughness Rq of 100 to 280 nm and a linear density of 1.6 to 10 per 1 ?m length.

Abstract: The present invention relates to a cell culture support for culturing mesenchymal stem cells, which includes en upper surface including a plurality of wells, in which the upper surface has a root mean square roughness Rq of 100 to 280 nm and a linear density of 1.6 to 10 per 1 ?m length.

Abstract: A cell culture module includes a sealing member 10 sealably holding in an inner space 12 a cell culture carrier S made up of a porous body having a concave portion S1 for culturing cells; a water pressure supplying portion 20 supplying a water pressure onto a second surface Sb opposite to a first surface Sa having the concave portion S1 in the state that a culture medium is filled in the inner space 12; and a cell recovering portion 30 recovering from the inner space 12 the cells separated from the concave portion S1 by the water pressure onto the second surface Sb.

Abstract: A compound represented by general formula (I) below; (in the formula, A denotes B denotes R denotes H, NO2, NH2, NHCbz, Br, F, Cl or SO3Na2, and n is an integer of 1 to 4), and a process for producing the above compound characterised in that it includes a reaction between an activated ester and a t-butoxycarbonylaminoethylamine or an ?-amino acid derivative.

Abstract: A colony (cell mass) proliferated under the undifferentiated state is obtained by using a carrier for cell culture in which two or more of a concavity having a porous body in a surface are arranged on a substrate surface in the form of a matrix, inoculating an undifferentiated cell on at least one concavity of the carrier for culture and carrying out culture.

Abstract: A compound represented by the general formula (I) below: In the formula, A is as defined in the specification and B denotes where n is an integer of 1 to 4, and a process for producing the above compound includes a reaction between an activated ester and a t-butoxycarbonylaminoethylamine or an ?-amino acid derivative.