Abstract: A transport container is configured to capture air bubbles remaining in a container with a simple mechanism and a simple operation to protect a graft. The transport container includes: a lid that seals a container for housing a graft, in which the lid has a protrusion protruding toward a space inside the container in a state of being attached to the container. A substantially annular groove is formed on a lower surface of the protrusion. Gas and liquid inside the container housing the graft and liquid are pushed out by the lid to achieve a liquid-tight state, and air bubbles in the liquid can be captured in the groove on the lower surface.

Abstract: A sheet-shaped cell culture for covering a cut surface of a pancreas, the cut surface of the pancreas includes cut surfaces of a pancreatic parenchyma and a pancreatic duct, and is connected to an intestinal wall of a small intestine in a liquid-tight manner via the sheet-shaped cell culture. A method for preventing or treating pancreatic fistula includes a step of covering, using a sheet-shaped cell culture, a cut surface of a pancreas that includes cut surfaces of a pancreatic parenchyma and a pancreatic duct, and a step of connecting the cut surface of the pancreas to an intestinal wall of a small intestine in a liquid-tight manner via the sheet-shaped cell culture.

Abstract: An object of the present invention is to provide a unit capable of efficiently and quickly detaching a graft from a device without causing damage or wrinkles. The above problem has been solved by a medical device that transfers a graft to a target site, the medical device including the graft and a support with the graft placed on one surface, in which the graft and the support are frozen in a state where the graft is placed, and the graft can be detached from the support and transferred to the target site by thawing the frozen graft placed on the support.

Abstract: A bioadhesive sheet-shaped material configured to be attached onto a surface of an organ, a method for producing the bioadhesive sheet-shaped material, and a method for treating a disease by using the bioadhesive sheet-shaped material. The bioadhesive sheet-shaped material includes an extracellular matrix layer, a sheet-shaped cell culture, and a biodegradable gel layer, where the sheet-shaped cell culture is interposed between the extracellular matrix layer and the biodegradable gel layer, and the bioadhesive sheet-shaped material is by attaching the extracellular matrix layer onto a surface of an organ.

Abstract: A container is disclosed for isolating living cells. The container for crushing a sample by pressing includes a container main body having an opening part and a partition which separates an upper space and a lower space of the container main body, in which at least a part of the partition has a portion which is not horizontal with respect to the bottom of the container.

Abstract: [Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation system 1 detects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.

Abstract: Systems and methods of the present disclosure include a cell culture, a method for producing the cell culture, a kit for producing the cell culture, a method for treating inflammatory disease using the cell culture, and the like, for treating inflammatory disease. The cell culture containing cells can be derived from skeletal muscle.

Abstract: A three-layer structure is comprised of an extra-cellular matrix, a sheet-shaped cell culture, and a biodegradable gel layer (single layer). By virtue of this three-layer structure, breakage of the sheet-shaped cell culture due to high spray pressure is reduced. The fibrin gel may be formed on one surface of the sheet-shaped cell culture by spraying of a thrombin liquid. A production method may involve culturing cells on a temperature-responsive cell culture substrate, dripping fibrinogen onto a sheet-shaped cell culture having an extra-cellular matrix on a lower surface thereof, further spraying a thrombin liquid onto the sheet-shaped cell culture from an oblique direction, whereby the result is a three-layer structure having a fibrin gel layer only on one surface of the sheet-shaped cell culture and having an extra-cellular matrix layer on the opposite surface.

Abstract: A device for efficiently attaching a sheet-shaped object to a target site includes a support body for supporting a sheet-shaped object, and a protective member having a low coefficient of friction for protecting one surface of the support, in which the support body supporting the sheet-shaped object is rolled while being protected by the protective member and can be inserted into a tubular body.

Abstract: Disclosed is a method for cryopreserving cells for transplantation capable of being suitably used for treatment of various diseases, a method for culturing the cells thawed after cryopreservation, a method for producing a graft containing a sheet-shaped cell culture containing the cells for transplantation, a graft produced by the production method, a composition and medical drug containing the graft, and a method for treating a disease using the graft. The cryopreservation method involves freezing a cell suspension containing cells for transplantation on a culture substrate. The method for culturing cells for transplantation includes incubation of a cell population in a medium containing a cryoprotective agent.

Abstract: Disclosed is a method for producing a high-quality graft from pluripotent stem cell-derived differentiation-induced cells, a graft produced by using the method, and a method for treating a disease using the graft are described Embodiments of a method for producing a graft are described, which include a step of performing an operation for removing undifferentiated cells in a cell population containing pluripotent stem cell-derived differentiation-induced cells, optionally a step of freezing the cell population and thereafter thawing the cell population, and a step of seeding the obtained cell population on a culture substrate and performing graft-forming culture.

Abstract: Disclosed are embodiments of a sheet-shaped cell culture for promoting healing of a lumen organ having a damaged part, particularly tissue of a duodenum, a method for producing a sheet-shaped cell culture. Also disclosed are embodiments of a method for regenerating a damaged part in a lumen organ using a sheet-shaped cell culture described herein.

Abstract: A method for preparing differentiation-induced cells from embryoid bodies derived from pluripotent stem cells is provided. The method includes adding a protease to embryoid bodies. The protease disperses the embryoid bodies, and the protease has an enzyme activity in the range from 0.3 to 4.0 recombinant protease activity unit (rPU)/ml.

Abstract: A method for producing a cell culture containing desired cells is provided. The desired cells are induced to be differentiated from pluripotent stem cells. The method includes seeding a cell population by dispersing embryoid bodies obtained by inducing differentiation from the pluripotent stem cells to the desired cells. The embryoid bodies are dispersed at a density reaching confluence or higher.

Abstract: [Problem] To evaluate objective cell groups by estimating a mixing ratio of objective cell group without affecting treatment itself and its production process when the objective cell groups include plural kinds of cell groups having different attributes, and to provide an image analysis system and a culture management system capable of accurately perform a quality control and a production control with low cost. [Solution] The cell quality evaluation system 1 detects a feature amount in each cell easily analyzable from images, and estimates a mixing ratio of each of plural kinds of cell groups included in the objective cell groups based on a distribution of the detected feature amount and pre-recorded information of the feature amount. As the feature amount, the embodiment uses a migration speed of each cell, easily distinguishable by analyzing tracking of each cell from plural images in time series.

Abstract: A method is disclosed for cryopreservation of cardiocytes derived from pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow, the method maintaining the function of the cardiocytes derived from differentiated pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow, and yet reducing the possibility for tumorigenesis of undifferentiated pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow. A method is also disclosed for cryopreservation of cardiocytes derived from pluripotent stem cells or mesenchymal stem cells (derived from adipose tissue or bone marrow, the method including dissociating cells from a cell population which has been induced to differentiate into cardiocytes from pluripotent stem cells or mesenchymal stem cells derived from adipose tissue or bone marrow.