Abstract: The present invention relates to a transformant which uses Schizosaccharomyces pombe as a host into which a D-LDH gene derived from bacteria of the genus Pediococcus and a D-LDH gene derived from bacteria of the genus Lactobacillus are incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of the Schizosaccharomyces pombe host have been deleted or inactivated.

Abstract: The object is to provide an expression vector capable of expressing with a higher expression efficiency a protein derived from a foreign structural gene by genetic engineering using a yeast of the genus Schizosaccharomyces as a host, a cloning vector to produce the expression vector, a method for producing the expression vector, a transformant containing an expression cassette of the expression vector, a method for producing the transformant, and a method for producing a protein using the transformant. A cloning vector comprising a promoter capable of functioning in a yeast of the genus Schizosaccharomyces, a cloning site for introducing a foreign structural gene which is located downstream from the promoter and is regulated by the promoter, and ihc2 gene terminator of the yeast of the genus Schizosaccharomyces, and an expression vector comprising the promoter, the foreign structural gene and the ihc2 gene terminator.

Abstract: The present invention relates to a transformant which uses Schizosaccharomyces pombe as a host into which a D-LDH gene derived from bacteria of the genus Pediococcus and a D-LDH gene derived from bacteria of the genus Lactobacillus are incorporated and in which some of the genes in a group of pyruvate decarboxylase-encoding genes of the Schizosaccharomyces pombe host have been deleted or inactivated.

Abstract: Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant comprising 3 copies of a human lactate dehydrogenase gene that is introduced into a Schizosaccharomyces pombe host, wherein a gene encoding pyruvate decarboxylase 2 of the Schizosaccharomyces pombe host is deleted or inactivated.

Abstract: The object is to provide an expression vector capable of expressing with a higher expression efficiency a protein derived from a foreign structural gene by genetic engineering using a yeast of the genus Schizosaccharomyces as a host, a cloning vector to produce the expression vector, a method for producing the expression vector, a transformant containing an expression cassette of the expression vector, a method for producing the transformant, and a method for producing a protein using the transformant. A cloning vector comprising a promoter capable of functioning in a yeast of the genus Schizosaccharomyces, a cloning site for introducing a foreign structural gene which is located downstream from the promoter and is regulated by the promoter, and ihc2 gene terminator of the yeast of the genus Schizosaccharomyces, and an expression vector comprising the promoter, the foreign structural gene and the ihc2 gene terminator.

Abstract: Provided is a transformant which can produce lactic acid with a high productivity without requiring neutralization with an alkali and is excellent in both of lactic acid production capability and growth ability and its production process, and a method for producing lactic acid by using the transformant. A transformant containing from 3 to 5 copies of a lactate dehydrogenase gene derived from human and introduced into a Schizosaccharomyces pombe host, wherein a gene that is a part of a group of genes encoding pyruvate decarboxylase of the Schizosaccharomyces pombe host is deleted or inactivated. A transformant characterized by providing a cell concentration of at least 4.0 g (on a dry cell weight basis)/L of a culture broth prepared by inoculating cells, at an initial cell concentration of 0.

Abstract: Provided is a transformant of S. pombe mutant which can produce and collect ?-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus Schizosaccharomyces. The transformant of a S. pombe mutant of the present invention is a transformant of a Schizosaccharomyces pombe mutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a ?-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus Schizosaccharomyces, and is useful for transforming a yeast of the genus Schizosaccharomyces, an expression vector, a transformant, etc.

Abstract: Provided is a transformant which uses Schizosaccharomyces pombe as a host, into which one or more foreign genes selected from the group consisting of a phosphoenolpyruvate carboxykinase gene and a pyruvate carboxylase gene are incorporated, and in which pdc2 genes of the Schizosaccharomyces pombe host have undergone deletion or deactivation, and a method for manufacturing a dicarboxylic acid having 4 carbon atoms by using the transformant.

Abstract: Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.

Abstract: The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced into Schizosaccharomyces pombe as a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated.

Abstract: Provided is a transformant of S. pombe mutant which can produce and collect ?-glucosidase without requiring complicated separation steps, and a vector which is useful for transforming a yeast of the genus Schizosaccharomyces. The transformant of a S. pombe mutant of the present invention is a transformant of a Schizosaccharomyces pombe mutant which exhibits an increased Gsf activity and decreased or no pyruvyl transferase Pvg1 enzymatic activity, and is comprised of a structural gene sequence encoding a ?-glucosidase derived from a filamentous fungus, and a promoter sequence and a terminator sequence for expressing the structural gene in a chromosome or as an extrachromosomal gene. Further, the present invention relates to a cloning vector which is characterized by comprising a hsp9 gene promoter or an ihc1 gene promoter of a yeast of the genus Schizosaccharomyces, and is useful for transforming a yeast of the genus Schizosaccharomyces, an expression vector, a transformant, etc.

Abstract: Objects of the present invention are to provide a transformant which can produce lactic acid with high productivity without requiring neutralization with an alkali, a method for producing the same, and a method for producing lactic acid by using the transformant. Namely, they are a transformant, in which Schizosaccharomyces pombe is used as a host, a lactate dehydrogenase gene of Lactobacillus pentosus is introduced, and a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated; a method for producing the transformant; and a method for producing lactic acid by using the transformant.

Abstract: Provided is a method for producing lactic acid which does not require neutralization and crude purification associated therewith both of which give a high load to the environment. A method for producing lactic acid, which comprises subjecting glucose to lactic acid fermentation by using a fission yeast having a lactic acid fermentation ability and recovering lactic acid produced thereby, characterized in that a fermentation liquor created from an aqueous glucose solution by lactic acid fermentation is replaced with an aqueous glucose solution having a potassium ion concentration of at least 400 ppm to continue the lactic acid fermentation, and the replacement of the fermentation liquor with the potassium ion-containing aqueous glucose solution is performed at least once.

Abstract: The present invention relates to a transformant, containing a lactate dehydrogenase gene which is introduced into Schizosaccharomyces pombe as a host, in which a part of a gene cluster encoding a pyruvate decarboxylase in the Schizosaccharomyces pombe host is deleted or inactivated.