Abstract: A protein assay method, including: 1) dissolving 4-hydroxybenzenesulfonic acid in an ethanol, methanol, or acetonitrile aqueous solution to prepare a hydrolysis reagent, adding a protein sample and the hydrolysis reagent to a hydrolysis tube, and uniformly mixing the protein sample and the hydrolysis reagent, charging argon or nitrogen into the hydrolysis tube to remove dissolved oxygen, sealing and drying the hydrolysis tube, and hydrolyzing the protein sample; 2) cooling the hydrolysis tube to room temperature, filtering and adding a resulting hydrolysate to a volumetric flask, washing the hydrolysis tube and a filter paper, collecting and adding a resulting washing solution to the volumetric flask to a constant volume; 3) drawing a hydrolysate solution from the volumetric flask, completely drying the hydrolysate solution to yield a solid product; and 4) diluting the solid product using a diluent, and analyzing amino acids of the solid product.

Abstract: A protein assay method, including: 1) dissolving 4-hydroxybenzenesulfonic acid in an ethanol, methanol, or acetonitrile aqueous solution to prepare a hydrolysis reagent, adding a protein sample and the hydrolysis reagent to a hydrolysis tube, and uniformly mixing the protein sample and the hydrolysis reagent, charging argon or nitrogen into the hydrolysis tube to remove dissolved oxygen, sealing and drying the hydrolysis tube, and hydrolyzing the protein sample; 2) cooling the hydrolysis tube to room temperature, filtering and adding a resulting hydrolysate to a volumetric flask, washing the hydrolysis tube and a filter paper, collecting and adding a resulting washing solution to the volumetric flask to a constant volume; 3) drawing a hydrolysate solution from the volumetric flask, completely drying the hydrolysate solution to yield a solid product; and 4) diluting the solid product using a diluent, and analyzing amino acids of the solid product.