Patents by Inventor Gabriel A. Kwong

Gabriel A. Kwong has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10883998
    Abstract: The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Grant
    Filed: April 30, 2018
    Date of Patent: January 5, 2021
    Assignee: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, Geoffrey A. von Maltzahn, Gabriel A. Kwong
  • Publication number: 20200299749
    Abstract: The disclosure provides methods and compositions to digitally profile biological activity by detecting the activity of at least two biomarkers using protease activity sensors or biocomparators.
    Type: Application
    Filed: April 27, 2020
    Publication date: September 24, 2020
    Inventors: Gabriel Kwong, Anirudh Sivakumar, Quoc Mac, Brandon Holt
  • Publication number: 20200299686
    Abstract: Embodiments of the present disclosure relate generally to compositions and methods for the design of remote controlled biological systems, and more specifically to synthetic bioswitches that provide the ability to non-invasively and remotely control the function and activity of live cells, such as for example and not limitation, the expression of biologically active proteins or biological therapeutics, and the manipulation of physiologic or genetic processes and/or protein expression in live cells, in vivo (including, e.g., at desired anatomical sites) or ex vivo.
    Type: Application
    Filed: October 2, 2018
    Publication date: September 24, 2020
    Inventors: Gabriel A. Kwong, Ian C. Miller, Marielena Gamboa Castro
  • Publication number: 20200249229
    Abstract: The invention provides methods and compositions that use a process in the body to deliver an active form of the activity sensor to a target site within the body. The activity sensors, which release detectable reporters when acted on by certain enzymes within the body, are provided as pro-analytes. Processes within the body deliver the activity sensors in active form to a target site of interest. For example, enzymes or a chemical environment within the body may cleave blocking groups from the activity sensors, or the body's tissues and organs may collect the activity sensors at the target site based on size or composition of the activity sensors.
    Type: Application
    Filed: January 30, 2020
    Publication date: August 6, 2020
    Inventors: Sangeeta Bhatia, Gabriel Kwong, Eric Huang, Sirshendu Roopom Banerjee, Andrew David Warren, Sophie Cazanave
  • Publication number: 20200246489
    Abstract: Methods of the present invention predict a physiological state of a subject. A composition comprising activity sensors is introduced into a body of a subject. The activity sensors comprise a plurality of reporters susceptible to cleavage when processed by the body during extracellular matrix remodeling. Cleavage may be indicative of enzymatic activity in the extracellular matrix. Signals detected by the activity sensors may be predictive of a physiological state. A sample is collected from the subject, and liberated reporters are detected in the sample. An onset of the physiological state of the subject, which may be a disease characterized by inflammation or atrophy, may be diagnosed based on the liberated reporters detected.
    Type: Application
    Filed: January 30, 2020
    Publication date: August 6, 2020
    Inventors: Sangeeta Bhatia, Gabriel Kwong, Eric Huang, Sirshendu Roopom Banerjee, Andrew David Warren, Sophie Cazanave
  • Publication number: 20200245926
    Abstract: The present invention provides method for longitudinal monitoring of disease changes and/or drug response. At multiple time points, a composition is introduced into a body of a subject (the composition includes an activity sensor comprising a plurality of reporters liberated through enzymatic cleavage, which occurs in a tissue of the body when enzymes associated with known activity of a therapeutic intervention are present in the body) and reporters are detected to determine activity levels of disease-associated enzymes in the subject, which is correlated to changes in disease state and/or drug response. A report or therapeutic response profile may further be used to support a clinical trial and for longitudinal monitoring of disease progression or regression in a subject. A prospective or active clinical trial participant, or patient undergoing treatment, may be qualified, stratified or staged based on the determined activity levels.
    Type: Application
    Filed: March 15, 2019
    Publication date: August 6, 2020
    Inventors: Sangeeta Bhatia, Gabriel Kwong, Eric Huang, Sirshendu Roopom Banerjee, Andrew Warren, Sophie Cazanave
  • Publication number: 20200225231
    Abstract: The invention relates to methods and products associated with in vivo enzyme profiling. In particular, biomarker nanoparticles capable of quantitatively detecting enzymatic activity in vivo are described. These nanoparticles can be used to detect in vivo enzyme activity. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Application
    Filed: November 22, 2019
    Publication date: July 16, 2020
    Applicant: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, David K. Wood, Gabriel A. Kwong, Andrew David Warren, Kevin Y. Lin
  • Patent number: 10527619
    Abstract: The invention relates to methods and products associated with in vivo enzyme profiling. In particular, biomarker nanoparticles capable of quantitatively detecting enzymatic activity in vivo are described. These nanoparticles can be used to detect in vivo enzyme activity. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Grant
    Filed: June 6, 2014
    Date of Patent: January 7, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, David K. Wood, Gabriel A. Kwong, Andrew D. Warren, Kevin Y. Lin
  • Publication number: 20190375793
    Abstract: An engineered cyclic peptide provides structural constraints to resist non-specific degradation in the human body and includes environment-specific cleavage sites to allow release of a linearized peptide upon reaching a target environment. The linearized peptide can include a reporter molecule or a bioactive therapeutic such that the cyclic peptide is essentially inactive at administration and in circulation but becomes reactive only upon exposure to target-specific environmental factors such as a specific combination of differentially-expressed proteases associated with a target tissue or disease state. The peptides can include tuning that modulate distribution by targeting the particle to specific tissue, bodily fluids, or cell types.
    Type: Application
    Filed: June 7, 2019
    Publication date: December 12, 2019
    Inventors: Faycal Touti, Gabriel Kwong
  • Publication number: 20190375796
    Abstract: An engineered cyclic peptide provides structural constraints to resist non-specific degradation in the human body and includes environment-specific cleavage sites to allow release of a linearized peptide upon reaching a target environment. The linearized peptide can include a reporter molecule or a bioactive therapeutic such that the cyclic peptide is essentially inactive at administration and in circulation but becomes reactive only upon exposure to target-specific environmental factors such as a specific combination of differentially-expressed proteases associated with a target tissue or disease state. The peptides can include tuning that modulate distribution by targeting the particle to specific tissue, bodily fluids, or cell types.
    Type: Application
    Filed: June 7, 2019
    Publication date: December 12, 2019
    Inventors: Faycal Touti, Gabriel Kwong
  • Publication number: 20190376113
    Abstract: A nanoparticle activity sensor containing a reporter and at least one tuning domain that modifies a distribution or residence time of the activity sensor when administered to a patient. When administered to the patient, the activity sensor enters cells or tissue where it is cleaved by enzymes specific to a physiological state such as a disease to release a detectable analyte. The tuning domains include molecular structures that modulate distribution or decay by protecting the particle from premature cleavage and indiscriminate hydrolysis, shielding the particle from immune detection and clearance, or by targeting the particle to specific tissue, bodily fluids, or cell types.
    Type: Application
    Filed: March 15, 2019
    Publication date: December 12, 2019
    Inventors: Sangeeta Bhatia, Gabriel Kwong, Eric Huang, Sirshendu Roopom Banerjee, Andrew Warren, Sophie Cazanave
  • Publication number: 20190376114
    Abstract: Methods and compositions for screening or diagnosis of liver disease are provided. The results may also indicate course and efficacy of treatment. The composition includes an activity sensor and a reporter releasably attached to the activity sensor. The method includes detecting the presence and/or amount of a reporter. The reporter is released from an activity sensor in the presence of diseased liver tissue but remains attached to the activity sensor in healthy tissue. The presence and/or amount of the reporter are used to characterize the liver disease. The liver disease may be nonalcoholic steatohepatitis (NASH) and results may further indicate staging of NASH.
    Type: Application
    Filed: March 15, 2019
    Publication date: December 12, 2019
    Inventors: Sangeeta Bhatia, Gabriel Kwong, Eric Huang, Sirshendu Roopom Banerjee, Andrew Warren, Sophie Cazanave
  • Publication number: 20190376115
    Abstract: Methods of the disclosure provide an analytical pipeline for mapping activity in a disease-specific manner. Any of a variety of diseases or medical conditions may be mapped using the analytical pipeline. In preferred embodiments, the pipeline uses expression data (e.g., from RNA-Seq) to identify proteases that are active in disease tissue and subject to differential expression relative to normal tissue. A machine learning classifier selects a subset of the proteases that identify the disease with a threshold sensitivity and specificity, in which the subset is small enough that a corresponding set of protease substrates may be assembled into a nanoparticle activity sensor that, when administered to a patient, are cleaved in the presence of disease tissue to release detectable analytes signifying presence of the disease.
    Type: Application
    Filed: June 7, 2019
    Publication date: December 12, 2019
    Inventors: Sangeeta Bhatia, Gabriel Kwong, Eric Huang, Sirshendu Roopom Banerjee, Andrew Warren, Sophie Cazanave
  • Publication number: 20190345534
    Abstract: An activity-based nanosensor composition for detecting protease activity comprising a cleavable detectable substrate and methods of use are disclosed.
    Type: Application
    Filed: September 28, 2017
    Publication date: November 14, 2019
    Inventors: Gabriel A. Kwong, Andrew B. Adams, Quoc Mac, David V. Mathews
  • Publication number: 20190144917
    Abstract: The present disclosure relates to methods and products associated with in vitro and in vivo protease activity measurements and enzyme profiling. Some aspects of the present disclosure relate to measuring remotely triggered protease activity. In particular, the disclosure relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence or absence of active enzymes associated with disease or conditions. The disclosure also relates to products, kits, and databases for use in the methods of the disclosure.
    Type: Application
    Filed: May 5, 2017
    Publication date: May 16, 2019
    Applicant: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, Gabriel A. Kwong, Piyush K. Jain, Jaideep S. Dudani, Simone Schurle
  • Publication number: 20180328941
    Abstract: The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Application
    Filed: April 30, 2018
    Publication date: November 15, 2018
    Applicant: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, Geoffrey A. von Maltzahn, Gabriel A. Kwong
  • Publication number: 20180196058
    Abstract: Some aspects of this invention provide reagents and methods for the sensitive, quantitative and simultaneous detection of target analytes in complex biological samples by liquid chromatography tandem mass spectrometry (LC MS/MS). Some aspects of this invention provide affinity reagents encoded with mass reporters for the sensitive and quantitative translation of an analyte of interest into a mass tag. The reagents and methods provided herein have general utility in analyte detection and encoding, for example, in biomolecular profiling, molecular diagnostics, and biochemical encoding.
    Type: Application
    Filed: December 14, 2017
    Publication date: July 12, 2018
    Applicant: Massachusetts Institute of Technology
    Inventors: Gabriel A. Kwong, Sangeeta N. Bhatia
  • Patent number: 10006916
    Abstract: Some aspects of this invention provide reagents and methods for the sensitive, quantitative and simultaneous detection of target analytes in complex biological samples by liquid chromatography tandem mass spectrometry (LC MS/MS). Some aspects of this invention provide affinity reagents encoded with mass reporters for the sensitive and quantitative translation of an analyte of interest into a mass tag. The reagents and methods provided herein have general utility in analyte detection and encoding, for example, in biomolecular profiling, molecular diagnostics, and biochemical encoding.
    Type: Grant
    Filed: March 15, 2012
    Date of Patent: June 26, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: Gabriel A. Kwong, Sangeeta N. Bhatia
  • Patent number: 9970941
    Abstract: The present invention relates to methods and products associated with in vivo enzyme profiling. In particular, the invention relates to methods of in vivo processing of exogenous molecules followed by detection of signature molecules as representative of the presence of active enzymes associated with diseases or conditions. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: May 15, 2018
    Assignee: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, Geoffrey A. von Maltzahn, Gabriel A. Kwong
  • Publication number: 20140363833
    Abstract: The invention relates to methods and products associated with in vivo enzyme profiling. In particular, biomarker nanoparticles capable of quantitatively detecting enzymatic activity in vivo are described. These nanoparticles can be used to detect in vivo enzyme activity. The invention also relates to products, kits, and databases for use in the methods of the invention.
    Type: Application
    Filed: June 6, 2014
    Publication date: December 11, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: Sangeeta N. Bhatia, David K. Wood, Gabriel A. Kwong, Andrew D. Warren, Kevin Y. Lin