Abstract: The invention relates to a cell culture system comprising a culture chamber comprising a flow compartment and a culture compartment being separated by a membrane, wherein the membrane is permeable to gas and is acoustically transparent, at least one inlet and one outlet to the flow compartment, at least one inlet and one outlet to the culture compartment; an acoustic actuation device comprising at least a first element being an ultrasonic transducer and an opposed second element being a reflector or a second ultrasonic transducer, wherein the acoustic actuation device is arranged to generate an acoustic field in the culture compartment. The invention also relates to a method for culturing cells using the cell culture system of the invention.

Abstract: A method is provided for manipulating objects in a cavity including a liquid, the method including providing in at least one region of the cavity objects capable of absorbing light in a given wavelength range, forming an aggregate of the objects by submitting them to an acoustic field, and disrupting the aggregate by submitting the aggregate to a light beam emitting at the given wavelength range. Also provided is a device for manipulating objects.

Abstract: A method is provided for manipulating objects in a cavity including a liquid, the method including providing in at least one region of the cavity objects capable of absorbing light in a given wavelength range, forming an aggregate of the objects by submitting them to an acoustic field, and disrupting the aggregate by submitting the aggregate to a light beam emitting at the given wavelength range. Also provided is a device for manipulating objects.