Abstract: A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5? terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5? terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

Abstract: A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5? terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5? terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

Abstract: A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5? terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5? terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

Abstract: A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5? terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5? terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

Abstract: A method for the preparation of a eukaryotic organism, for example selected from plants, animals and fungi, showing constitutive, inducible and/or organ specific expression of a specifically modified TPS gene, which comprises the steps of providing a TPS gene; designing a suitable modification to the TPS gene by aligning the gene with the corresponding gene of yeast and establishing which part of the gene extends beyond the 5? terminus of the yeast gene; deleting or inactivating a part of the N-terminal region of the TPS gene extending beyond the 5? terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; cloning the thus modified gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; transforming a plant cell or tissue with the thus obtained expression vector; and regenerating a complete plant from the transformed plant cell or tissue.

Abstract: Transgenic plants or transgenic plant cells having increased stress resistance under environmental or artificial stress conditions are produced by a method comprising the steps of: a) transforming part of a plant or part of a plant cell population with a nucleotide sequence comprising (i) a first nucleotide sequence coding for a eukaryotic environmental or artificial stress resistance protein, or an allele or a homologue thereof, or a catalytically active part of the said protein, the said sequence being operably linked to a promoter, and (ii) one or more restriction sites comprising a second nucleotide sequence, b) applying environmental or artificial stress conditions to the transformed plant or plant cell population obtained in step (a), or its progeny, and c) selecting, from the transformed plant or plant cell population placed under the environmental or artificial stress conditions of step (b), that part of the transformed plant or plant cell population which exhibits an increased stress resistance under