Abstract: The present invention relates to a nanocell, and method for manufacturing same, for holding small volumes of soft matter confined in a gap of order 1 micrometer or smaller and measurement systems using the same. The nanocell comprise: a first structure (3) with a substantially flat surface in one direction; a second structure (6) with a substantially flat surface in one direction; and at least one spacer (1, 2); wherein said substantially flat surface of each first and second structure face each other and are separated by the at least one spacer and the two surfaces and the spacer together define a volume (7) between them for holding said soft matter or fluid, and the distance defined by the spacer between said surfaces of the first and second structure is less than 1 micrometer; the volume between the surfaces of the first and second structures and the spacer is in the range between 1 femto liters and 1 micro liters.