Abstract: A method for increased and permanent production of a natural compound by a microorganism during an exponential growth and during a stationary phase of a culture of the microorganism, the method comprising: enhancing a translation of a target mRNA, wherein the target mRNA comprises a transcript of a target gene, wherein the target gene is encoding the natural compound or a key enzyme involved in a biosynthesis of the natural compound in the microorganism, by replacing a wild type (wt) ribosome binding site (RBS) upstream of the target gene with a synthetic RBS, the synthetic RBS possessing a higher affinity towards a 16S rRNA of a ribosome of the microorganism than the wt RBS; and enhancing a transcription of the target gene by converting an osmotically regulated ?38 promoter upstream of the target gene into a stationary ?38 promoter, wherein the osmotically regulated ?38 promoter comprises a ?35 G-element and a ?10 element which are separated from each other by a spacer sequence, by deleting the ?35 G elem