Abstract: The effect of the action of collagenase in treating lesions containing collagen or mixed fibrin clots (e.g., burns or ulcers) is improved by applying collagenase to the wound in combination with an organogel and a phosphatidylcholine, of which lecithin is a readily available source, particularly when the collagenase is first mixed and stored with that choline and an organic solvent and is subsequently mixed with a second individually stored formulation comprising a gel-forming agent and water, the two formulations individually having satisfactory shelf lives and the resultant mixture being applied to the lesion promptly after being formed.

Abstract: The effect of the action of collagenase in treating lesions containing collagen or mixed fibrin clots (e.g., burns or ulcers) is improved by applying collagenase to the wound in combination with an organogel and a phosphotidylcholine, of which lecithin is a readily available source, particularly when the collagenase is first mixed and stored with that choline and an organic solvent and is subsequently mixed with a second individually stored formulation comprising a gel-forming agent and water, the two formulations individually having satisfactory shelf lives and the resultant mixture being applied to the lesion promptly after being formed.

Abstract: The debridement of wounds, and particularly burn wounds, is more effectively accomplished by starting with a first substance comprising a mixture of collagenase and a non-aqueous excipient and a second substance comprising an aqueous excipient and, preferably, a cream such as an antibiotic cream, both of which substances have a satisfactory shelf life, and treating the wound by combining those two substances and promptly applying the resulting combination to the wound. Shelf life requirements are satisfied, and the effect of applying the resulting combination to the wound promptly after the combination has been formed results in a more effective debridement than would be expected from the application of other collagenase substances.