Abstract: The present invention relates to a long-acting recombinant GLP-1-Fc fusion protein containing CD47 signals and preparation process as well as use thereof. To be specific, the present invention relates to a novel recombinant fusion protein which includes GLP-1 polypeptides, Fc fragments and CD47 signal polypeptides. This fusion protein has been found for the first time to be able to exist as a tetramer, more stable than the homodimeric Fc fusion protein, and with effectively prolonged half-life in vivo. The present invention also mutates GLP-1 and Fc fragments to enhance GLP-1 biological activities and reduce antibody-dependent cell-mediated cytotoxicity (ADCC) effects so as to decrease potential cytotoxicities and immunogenicity. Furthermore, the initial combination of the CD47 signal polypeptide with the fusion protein in this fusion protein can reduce macrophage clearance of the polypeptide and decrease dosing frequency in humans.

Abstract: A recoverable instant thickening acid and its reusing method is usable for the recycling and reusing of a gas well carbonate reservoir acidizing fluid and production stimulation operation. The recoverable instant thickening acid includes the following components in weight percentage: 1.5-3 parts of a thickener, 0.5-3 parts of potassium chloride and 100 parts of a hydrochloric acid solution, wherein the thickener is a mixture composed of a mass ratio of 40%-70% of N-lauryl-N?-hydroxyethyl-N?-hydroxypropyl ethylenediamine propionate or its derivatives, 4%-12% of ethanol and 18%-56% of water. With the thickening acid for performing an acidizing treatment, the residual acid solution can be re-prepared as a thickening acid for an acidizing treatment without a chemical treatment, assisting the green and environmentally friendly production and sustainable development of gas fields.

Abstract: A recoverable instant thickening acid and its reusing method is usable for the recycling and reusing of a gas well carbonate reservoir acidizing fluid and production stimulation operation. The recoverable instant thickening acid includes the following components in weight percentage: 1.5-3 parts of a thickener, 0.5-3 parts of potassium chloride and 100 parts of a hydrochloric acid solution, wherein the thickener is a mixture composed of a mass ratio of 40%-70% of N-lauryl-N?-hydroxyethyl-N?-hydroxypropyl ethylenediamine propionate or its derivatives, 4%-12% of ethanol and 18%-56% of water. With the thickening acid for performing an acidizing treatment, the residual acid solution can be re-prepared as a thickening acid for an acidizing treatment without a chemical treatment, assisting the green and environmentally friendly production and sustainable development of gas fields.